两种洗涤方法对马传染琼扩抗原生产试验

用两种不同洗涤方法生产马传染琼扩抗原。试验结果表明,用硫酸重铬酸钾清洗液洗刷的组织培养克氏瓶,细胞生长旺介线清楚,立体感强,接种马传染性贫血病毒后。细胞抗原产量高于用清洁剂刷的克氏瓶培养的细胞抗原的２￣３倍。     

自然感染日本血吸虫病耕牛血清循环抗原消长规律的研究

为了观察疫区自然感染日本血吸虫病耕牛血清循环抗原消长规律,本试验将血吸虫病非疫区的水、黄牛各１０头,转运至安徽省血吸虫病疫区,接受为期８周的自然感染。用斑点酶联免疫吸附试验测定牛血清循环抗原。结果,水、黄牛血清循环抗原滴度均于自然感染第４周后明显上升,水、黄牛自然感染第８周的血清循环抗原最高滴度分为１∶１０２４０和１∶５１２０,最低滴度则分别为１∶１２８０和１∶６４０,水牛循环抗原滴度高于黄牛。虫荷数与血清循环抗原滴度未见有相关性。     

The role of toll-like receptors in the pathogenesis of Streptococcus suis

Streptococcus suis is an important agent of swine and human meningitis. Sequence type (ST) 7 emerged in China and was responsible for the human epidemic caused by S. suis in 2005. The virulence of S. suis ST7 is greater than the wild type pathogenic S. suis, ST1; however, the mechanisms for this increased pathogenicity are unknown. The aim of this study was to determine the role of different toll-like receptors (TLRs) involved in regulating the host response to the S. suis infection and to speculate on differing mechanisms used by ST7 strains to induce disease. Here we compared two ST7 strains isolated in the 2005 Sichuan outbreak to two ST1 strains. Our data show TLR2, 6 and 9 are involved in the recognition of heat-killed S. suis independent of the ST type. We found the TLR-dependent cytokine production differed between the two types of strains using whole cell lysate proteins. TLR6 played a greater role in cytokine production induced by the whole cell lysate proteins from the ST7 strain than in that induced by the ST1 strain lysates. The data suggest that mechanisms of inflammation induced by S. suis strains differ where this will be useful in designing efficient strategies in combating streptococcal toxic shock-like syndrome caused by the S. suis ST7 strains.     

二月兰与杜鹃花共建百里杜鹃景区景观时空观赏性

百里杜鹃景区花期过于集中,花期大多在4—5月,旅游期短。现以二月兰与优势种马缨杜鹃为试材,通过在马缨杜鹃树下配种二月兰,研究二月兰是否增加景区的观赏效果,同时,研究引种二月兰是否对马缨杜鹃的生长造成不良影响。结果表明:在马缨杜鹃树下种植二月兰并不影响其生长;二月兰的花期为3—5月,株高多分布在50~100cm,与马缨杜鹃相比,在群落上属于下层景观;这使得景区的可观赏时间提前了将近1个月,同时增强景区空间上的观赏效果。     

北京龙耘种业有限公司

中单28是中国农科院作物所选育的高产、多抗、优质、中晚熟普通玉米单交种，其组合为中1128x中5493。2005年4月经北京市农作物品种审定，审定编号为：京审玉2005001。2007年3月经山西省农作物品种审定，审定编号为：晋引玉2007008。     

福建省将乐县金溪建电站前后雨水情变化分析

本文通过对将乐县金溪建电站前的1953～1978年、建电站后的1990～2009年雨水情进行分析,结果表明:自然状态下的将乐县(1953～1978年)无论雨水大小,每年都有洪水超过警戒水位,年最大洪水发生时间在4～7月;1953～1978年有16年的当年最大洪峰水位发生在当年最大日降雨量当日至第16日内,频率为61.5...     

地表火对宝天曼栎类森林生态系统服务功能的影响研究

2008年4月至2010年6月采用时间序列代替空间序列的方法,对宝天曼栎类森林生态系统服务功能受地表火影响在不同时间的涵养水源、保育土壤、固碳释氧、生物保育、积累营养物质、负离子提供和森林病虫害防治等7个方面进行了初步评估。结果表明,地表火干扰造成宝天曼栎类森林生态系统服务功能损失的价值在时间序列上依次为1年是24.95万元.hm-2,5年是11.68万元.hm-2,10年是4.91万元.hm-2,15年是0.68万元.hm-2;各项服务功能价值量损失排序为保育土壤>涵养水源>病虫害防治>固碳释氧>积累营养物质>净化大气>生物保育,其中保育土壤和涵养水源两项的损失占总损失量的70%左右,这表明地表火主要影响宝天曼栎类森林生态系统中的保育土壤和涵养水源功能。     

国外生猪育种体系简析及对我国生猪育种的几点思考

选择是生猪育种的核心,而准确地选择和选种依赖于完善和成熟的育种体系。国际养猪发达国家(国际育种企业)对育种体系不断完善,长期进行生长、繁殖和肉品质等各类性状的表型测定,运用多场联合评估和基因组选择等方法不断提高遗传评估的准确性,不断挖掘新的育种性状,并进行测定、评估和选育,实现了种猪群体持续的遗传改良和综合性能的不断提升。本文对欧美发达国家(国际育种企业)的生猪育种技术体系进行了综述,以期为我国的生猪育种工作提供参考,并促进我国生猪种业的创新发展。     

Inhibition of porcine circovirus type 1 and type 2 production in PK-15 cells by small interfering RNAs targeting the Rep gene

Porcine circovirus type 1 (PCV1) and type 2 (PCV2) are two genotypes of porcine circovirus. Both of them are presumed to be widespread in the swine population. Currently, there is no specific treatment for their infections. RNA interference (RNAi) is a sequence-specific RNA degradation mechanism mediated by small interfering RNA (siRNA), which represents a possible therapeutic application for the treatment of viral infections. In this study, three siRNA expression plasmids (pS-RepA, pS-RepB and pS-RepC) were generated to target three different coding regions of the Rep protein (Rep) of PCV. These siRNAs were used to inhibit PCV production in a porcine kidney cell line, PK-15 cells. Our results revealed that Rep gene expression was inhibited by pS-RepA, pS-RepB and pS-RepC to different degrees. Moreover, our study also showed that the production of PCV1 and PCV2 was reduced by these siRNAs. pS-RepC, which targets the middle region of Rep gene, proved to be the most efficient siRNA for inhibition of Rep expression and viral production. Taken together, our data suggest that RNAi could be investigated as a potential treatment for PCV infection.     

A Method of Adaptive Fuzzy Classfication on Rockmass Blast Ability

This paper presents a series of targets which are usefull for engineering.The paper also presents an adaptive weight function according to the fail mechanism of rockmass and a fuzzy aynthetic evaluation medel on rockmass blast ability.The model is applied to evaluate the blast ability of rockmass in an engineering and the result shows that the model is effective.