Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period

The endometrium of sheep consists of plenty of raised aglandular areas called caruncular (C), and intensely glandular intercaruncular areas (IC). In order to better understand the endometrium involved mechanisms of implantation, we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period, and then compared the proteomic profiles between these two areas. We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively. By comparing the proteome of these two areas, we found 170 differentially expressed proteins (DEPs) (P < 0.05), functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue, cell adhesion and protein transport, and so on. Our study, for the first time, provided a proteomic reference for elucidating the differences between C and IC areas, as an integrated function unit respectively, during the peri-implantation period. The results could help us to better understand the implantation in the ewes. In addition, we established a relatively detailed protein database of ovine endometrium, which provide a unique reference for further studies.     

《青海畜牧兽医杂志》（1992—1998）论文及作者统计分析

用文献计量方法对《青海畜牧兽医杂志》国内外公开发行后７年间所刊载的论文及其作者进行了统计分析与探讨。主要项目有：论文数量及年度分布、论文学科内容分布、论文地域分布、论文机构分布、单位平均产文量、论文合著率、作者数量及地区分布、作者系统及单位分布、作者个人发文量、作者及单位合作度等。另外还确定出《杂志》１９９２～１９９８年的核心作者和核心单位。     

奶牛热应激及日粮阴阳离子平衡对其影响

日粮阴阳离子平衡(Dietary Cation Anion Balance,DCAB)已成为奶牛营养学研究的一个新方向,它直接影响到奶牛业的生产和经济效益。文中综述了热应激奶牛生产性能、繁殖性能和免疫机能等方面的变化以及DCAB对热应激奶牛的生产性能、机体酸碱平衡和奶牛健康的影响。     

甲鱼暴发性传染病的病原分离及治疗试验

从１５例表现为“红底板”、“红脖子”、“烂甲”的患病甲鱼肝脏分离到９株无荚膜、无芽胞、革兰氏阴性、β溶血的短杆菌,经理化特性鉴定均为气单胞菌属（Ａｅｒｏｍｏｎａｓ）嗜温有动力菌群,其中温和气单胞菌（Ａ．ｓｏｂｒｉａ）７株,豚鼠气单胞菌（Ａ．ｃａｖｉａｅ）２株。从Ａ．ｓｏｂｒｉａ和Ａ．ｃａｖｉａｅ各选一代表菌株（ＪＡ－１和ＪＡ－２）分别感染健康鲫鱼均引起发病和死亡,其ＬＤ５０分别为４．３×１０５、５．５×１０７,并回收到该２种菌。在该２种菌培养上清中存在１种具有溶血活性的毒素。以ＪＡ－１和ＪＡ－２为菌种制成混合菌苗,在应用由黄芪、有机锗制成的免疫增效剂后,再用混合菌苗免疫家兔,制得的多价高免血清（效价达１∶５１２）与庆大霉素、恩诺沙星进行了治疗对比试验。结果,高免血清治疗自然病甲鱼１０９只,全部治愈;庆大霉素、恩诺沙星分别治疗自然病甲鱼７２只和８４只,平均治愈率分别为８８％和８４％。     

环保畜牧业与健康养殖发展探讨

当今,养殖业面对的环保要求越来越高,且人类社会对畜禽产品的质量也是高度关注。本文从畜牧业污染产生的危害、治理思路、控污的具体措施以及健康的养殖模式展开分析,并做出一些对未来养殖业发展的预测,旨在为环保养殖提供参考与借鉴。     

和胃平喘对小鼠肠绒毛高度的影响

选择大小、体重相近的SD雌性小鼠(18～22g)40只,随机分为4组,1.5%和胃平喘组、2.5%和胃平喘组、3.5%和胃平喘组和基础料组,每组2个重复,每个重复5只,分别饲喂15d和30d后取肠道做成切片,测定小鼠肠道的肠绒毛高度。结果表明:15d试验中,1.5%和胃平喘组、2.5%和胃平喘组、3.5%和胃平喘组与对照组间的平均肠绒毛高度差异显著(P0.05)。30d试验中,2.5%和胃平喘组十二指肠平均肠绒毛(duodenum villus)高度为0.9835±0.1582mm,3.5%和胃平喘组十二指肠平均肠绒毛高度为0.8898±0.1635mm,2.5%和胃平喘组、3.5%和胃平喘组的十二指肠平均肠绒毛高度都与对照组的差异极显著(P0.01),1.5%和胃平喘组的十二指肠平均肠绒毛高度低于对照组,但差异不显著(P0.05);2.5%和胃平喘组和3.5%和胃平喘组的空肠平均肠绒毛(jejunum villus)高度与对照组间的差异不显著(P0.05);1.5%和胃平喘组和2.5%和胃平喘组的回肠平均肠绒毛(ileum villus)高度与对照组间的差异不显著(P0.05)。在小鼠基础料中添加和胃平喘能不同程度提高小鼠肠绒毛高度,且以2.5%和3.5%两个添加量效果最好。     

灭虫精对西施舌幼贝的毒性试验

在静水条件下,进行灭虫精对西施舌幼贝的毒性试验,试验结果显示:灭虫精对西施舌幼贝24 h致死浓度LC100为400 ms／L,半数致死浓度LC100为250 mg/L,安全浓度为2．50mg／L;48h致死浓度为260 mg／L,半数致死浓度LC50为224ms／L,安全浓度为2．24mg／L。灭虫精用于西施舌人工育苗过程中桡足类等敌害生物防治,具有较强的可用性。根据试验结果,建议使用2 mg／L浓度的灭虫精进行防治,可达到显著效果。     

Inhibition of porcine circovirus type 1 and type 2 production in PK-15 cells by small interfering RNAs targeting the Rep gene

Porcine circovirus type 1 (PCV1) and type 2 (PCV2) are two genotypes of porcine circovirus. Both of them are presumed to be widespread in the swine population. Currently, there is no specific treatment for their infections. RNA interference (RNAi) is a sequence-specific RNA degradation mechanism mediated by small interfering RNA (siRNA), which represents a possible therapeutic application for the treatment of viral infections. In this study, three siRNA expression plasmids (pS-RepA, pS-RepB and pS-RepC) were generated to target three different coding regions of the Rep protein (Rep) of PCV. These siRNAs were used to inhibit PCV production in a porcine kidney cell line, PK-15 cells. Our results revealed that Rep gene expression was inhibited by pS-RepA, pS-RepB and pS-RepC to different degrees. Moreover, our study also showed that the production of PCV1 and PCV2 was reduced by these siRNAs. pS-RepC, which targets the middle region of Rep gene, proved to be the most efficient siRNA for inhibition of Rep expression and viral production. Taken together, our data suggest that RNAi could be investigated as a potential treatment for PCV infection.     

对奶牛胎衣不下的认识和处理

本文结合本场的生产实际，讨论了母牛胎盘的特点，胎衣不下的症状与诊断；着重讨论了本病的发病率及其影响因素，并将病因分析、归纳成一个示意图；最后综述了多项治疗和预防胎衣不下的措施，如灌服羊水，注射激素，用维生素A、D、E和亚硒酸钠，灌服中药，手术剥离以及电刺激等。     

乳源性生长因子和初生动物胃肠道生长发育的关系

对３ 种主要乳源性生长因子： 表皮生长因子, 胰岛素样生长因子和胰岛素的结构、性质、主要功能、可能的作用机制及目前的研究进展进行了综述。３ 种生长因子都可以在初生动物胃肠道中相对稳定地存活, 对动物胃肠道生长发育、消化道粘膜细胞的增殖和分化及消化酶的产生均有不同程度的促进作用。