野生猕猴STLV-1和B病毒抗体的检测

为了调查保护区野生猕猴是否感染猴的3种主要病毒,从广西某自然保护区一只濒死的野生猕猴抽取血液分离血清,分别应用猴3种病毒斑点酶免疫检测试剂盒检测,结果猴T淋巴细胞白血病病毒1型(STLV-1)和猕猴疱疹病毒I型(B病毒)抗体呈阳性,而猴免疫缺陷病毒抗体呈阴性.     

宁夏首起牛结节性皮肤病疫情流行病学调查

2020年10月,宁夏盐池县、利通区、平罗县发生输入性牛结节性皮肤病疫情。为查明疫情发生原因,分析疫情扩散风险,宁夏回族自治区农业农村厅畜牧兽医局组织成立督导组进行流行病学调查。通过现场调查、座谈及实验室检测,综合发病、溯源和追踪情况以及实验室检测结果,分析认为此次疫情是由调入纯种荷斯坦育成奶牛引起的。因及时采取了封锁疫点、控制移动、扑杀、无害化处理、清洗消毒、杀灭蚊蝇、免疫等综合处置措施,疫情扩散风险较低。此次疫情警示,要强化牛只及其产品的调运监管,加强疫情排查监测,及时发现和处置疫情。     

液相色谱-串联质谱法同时检测鸡肉中61种抗菌药物

为建立固相萃取结合液相色谱-串联质谱同时测定鸡肉中磺胺类、喹诺酮类、大环内酯类药物残留的分析方法,通过准确称取2 g鸡肉样品,经PBS缓冲液提取,以Agilent Pleax PCX固相萃取柱净化,最后以液相色谱-串联质谱进行检测.结果显示:在最优试验条件下测得磺胺类、喹诺酮类、大环内酯类抗菌药物线性良好,相关系数(R...     

HPLC法测定兽用中药散剂中非法添加利巴韦林的方法研究

建立了高效液相色谱法检测白龙散、黄连解毒散、苍术香连散等7种兽用中药散剂中非法添加利巴韦林的方法。用十八烷基硅烷键合硅胶色谱柱,以水（用稀硫酸调节pH值至2．5±0.1）为流动相,检测波长207nm,流速1．0mL／min,进样量20μL。结果显示,利巴韦林的线性范围为1.016～20.32μg／mL,线性方程为Y=5．68×10X-5．61×10^-2（r=0．9999,n=6）,本方法的回收率为81．28％～119．46％,RSD为0．88％～1．65％。结果表明,本方法准确、可靠,适用于兽用中药散剂中非法添加利巴韦林的检测。     

Application of non-structural protein ELISA kits in nationwide FMD surveillance in pigs to demonstrate virus circulation in Taiwan

Large scale surveillance of FMD non-structural protein (NSP) antibody in pigs was conducted to monitor for FMD virus circulation in Taiwan using Ceditest and UBI NSP ELISA kits after recurrence of FMD in 2009. A total of 53,759 serum samples were collected from pigs in the auction markets in 2009. There were 43 farms with positive FMD NSP reactors to both NSP ELISA tests in the nationwide surveillance. After tracing back, clinical examination and the NSP ELISA testing using both Ceditest and UBI on 14 follow-up serum samples from all the herds with confirmed NSP reactors in 2009, there were 4 farms classified as positive on follow-up testing criteria. In this surveillance, we have demonstrated that the NSP ELISA tests of outbreak farms followed by clinical and serological investigation could be used to detect FMD circulation in the pig population in Taiwan even while the national compulsory vaccination program is ongoing.     

粗糙对矿用排水泵轴功率的影响分析

矿用排水泵是矿山排水设备的重要组成成分之一,用于将采矿过程中随时涌入的矿井水排除.本文通过对矿井排水管路流动阻力损失的分析,说明了流动阻力损失与粗糙度之间的关系,并由矿用排水泵的扬程、有用功率、轴功率与流动阻力损失间的函数方程,反映出粗糙对矿用排水泵轴功率的影响.指出了矿山排水系统在使用中应当注意的问题,并提出了相应的处理措施.     

在体外模拟瘤胃发酵条件下N-乙酰-DL-蛋氨酸稳定性的研究

通过两个试验,研究在体外模拟瘤胃发酵条件下N-乙酰-DL-蛋氨酸的降解率。试验1:N-乙酰-DL-蛋氨酸在pH值6.5、温度为39℃的缓冲液中孵育9h,其降解率为3.3%。试验2:运用瘤胃体外发酵技术研究N-乙酰-DL-蛋氨酸不同添加量对pH值、氨态氮、微生物蛋白动态变化的影响。结果表明,添加0.4、0.6gN-乙酰-DL-蛋氨酸发酵3～6h,均能够显著降低体系的pH值;添加0.6gN-乙酰-DL-蛋氨酸发酵12h能够显著提高氨态氮的浓度;N-乙酰-DL-蛋氨酸在发酵过程中不影响微生物蛋白产量。以上结果显示,N-乙酰-DL-蛋氨酸在体外模拟瘤胃发酵的条件下可以稳定存在。     

蒸汽压片玉米加工工艺及其对牛生产性能的影响

蒸汽压片是一种对玉米进行湿热加工的加工工艺,可以提高玉米的生物学价值,改善其利用率,可以提高瘤胃淀粉、过瘤胃淀粉和总肠道淀粉消化率。同时还可以促进其它物质的消化代谢,减少氮、磷的排泄。经过严格质量控制的蒸汽压片玉米可以提高舍饲肉牛的肉料比和提高奶牛的产奶量和乳蛋白量。     

Effects of supplemental vitamin E during the mature period on the reproduction performance of Taiwan Native Chicken cockerels

One-day-old Taiwan native male chicks were fed with maize-soybean rearing diets without supplemental vitamin E to 23 weeks of age. From 23 to 52 weeks of age, the cockerels (n = 90) were assigned at random to 5 dietary treatments and fed with maize-soybean diets supplemented with 0, 20, 40, 80 and 160 mg/kg of vitamin E (dl-alpha-tocopherol acetate). Pullets (225) of the same age were fed with standard diets throughout. They were artificially inseminated with one dose of 0.04 ml/bird intact and 5-fold diluted pooled semen at 31 to 43 weeks of age and at 49 weeks of age, respectively. The criteria evaluated included: semen quality, fertility and maximum and effective duration of fertility, blood characteristics, body and testes weight. Supplemental vitamin E did not affect cockerels' effective duration of fertility and percentage of fertility. However, when pullets were inseminated with diluted semen, supplementing 160 mg/kg vitamin E increased the maximum duration of fertility at 49 weeks of age. Cockerels receiving 40 to 160mg/kg supplements had higher sperm viability and motility after 39 weeks of age and those fed 80 mg/kg had higher sperm concentration at 39 weeks of age. Cockerels receiving supplements of more than 40 mg/kg vitamin E had higher body weight gain. Plasma cholesterol and testosterone were not affected by supplemental vitamin E. However, plasma luteinising hormone (LH) concentration was lower in cockerels fed 160 mg/kg. Lack of supplemental vitamin E over 39 weeks was associated with lower semen quality but did not reduce the proportion of fertile eggs laid by inseminated hens, perhaps because the insemination dose compensated for low sperm quality. We found that the maximum duration of fertility might be improved by supplementing 160 mg/kg vitamin E at 49 weeks of age.     

甲酸钙的合成、测定及其在乳猪料中的应用

甲酸钙作为一种饲料添加剂,能降低和调节胃肠道pH值、促进营养物质的消化和吸收。本文系统介绍了甲酸钙的合成和检测方法,并介绍了甲酸钙作为饲料添加剂的作用效果。