可持续发展条件下高等农林教育功能的完善与拓展

阐述了在可持续发展中高等农林教育功能与特征,并从时代的角度,论述了可持续发展条件下高等农林教育的政治功能、经济功能、培养人才功能、文化功能、科学技术功能和社会智囊功能的完善与拓展。     

中国条锈菌新小种条中30、31号的研究

本文报道了1991年以来对新小种条中30、31号鉴定与致病性的研究。继1991年发现对绵阳系成株小麦有致病力的、对Hybrid46有毒的新致病类型91—1,1993年又发现了新的致病类型93—1。根据它们对我国鉴别寄主的反应,命名为条中30、31号。与条中28、29号相比,新小种具有更宽毒性基因组成和更高的相对寄生适合度值,它们对我国生产品种、高代品系和重要抗源有更广的致病范围。证实两个新小种的出现和发展是绵阳系小麦抗条锈变异的主要因素,建议加强对新小种抗病育种和流行预测的研究。     

Investigation of the In Vivo,In Vitro,and In Silico Wound Healing Potential of Pinctada martensii Purified Peptides

Previous studies found that both oral and topical administration of enzymatic digestion products < 3 K Da ultrafiltration fractions of Pinctada martensii mantle (PMPs) had pro-healing effects. Thus, we further purified them by Sephadex-G25 and screened them by cellular assays to obtain Pinctada martensii purified peptides (PMPPs). In this study, we explored the mechanism of PMPPs on wound healing by in vivo, in vitro, and in silico experiments. LC-MS/MS results showed that PMPPs consisted of 33 peptides with molecular weights ranging from 758.43 to 2014.04 Da, and the characteristic peptide was Leu-Asp. The results of cellular assays showed that PMPPs promoted the proliferation of human skin fibroblasts (HSF) (135%) and human immortalized keratinocyte (HaCaT) cells (125%) very significantly at 12.5 μg/mL. The in vivo results showed that PMPPs could achieve scarless healing by inhibiting the inflammatory response, accelerating the epithelialization process, and regulating collagen I/III ratio. The optimal peptide sequence FAFQAEIAQLMS of PMPPs was screened for key protein receptors in wound healing (EGFR1, FGFR1, and MMP-1) with the help of molecular docking technique, which also showed to be the key pro-healing active peptide sequence. Therefore, it may provide a therapeutic strategy with great potential for wound healing.     

侵染桃树的病毒、类病毒和植原体病害研究进展

目前国内外报道的桃树病毒病３３ 种,其中已分离和鉴定的病原病毒１５ 种;类病毒３ 种;植原体病害４ 种。分别概述了这些病害的种类、分布、症状、检测、传播以及防治策略等方面的研究进展     

Hemin induces and sustains the phosphorylation of Erk1/2 in human umbilical vascular endothelial cells

AIM: To investigate the relationship between hemin and Erk1/2 activation in human umbilical vascular endothelial cells (HUVECs). METHODS: Cultured HUVECs were separately incubated with hemin or H₂O₂ for different times. Subsequently Erk1/2 phosphorylation and total Erk1/2 were determined by Western blotting assay. Flow cytometry was employed to determine the cell cycle distribution. RESULTS: Hemin at the concentration of 1-10 μmol/L induced the phosphorylation of Erk1/2 in HUVECs, and sustained the phosphorylation of Erk1/2 for three hours. The duration of phospho-Erk1/2 induced by hemin was much longer than that in H₂O₂ control (3 h vs 30 min). CONCLUSION: Hemin induces and sustains the phosphorylation of Erk1/2 in HUVECs, which indicates that the effect of hemin on the Erk1/2 activation may be one of pharmacological target of hemin.     

增施氮肥和地面管理对酸樱桃园土壤营养状况的影响

在一个连续进行了 ８年Ｎ肥定位试验的酸樱桃园,调查了每年施用 Ｎ６０ ｋｇ· ｈｍ~(－２)和行间生草制对土壤营养状况的影响。结果表明：在肥沃土壤里ＮＨ_４~＋是速效Ｎ的主要存在形式,而ＮＯ_３~－的含量很低。生草地土壤中含有较多的 ＮＨ_４~－,而清耕带土壤中含有较多的 ＮＯ_３~－。施肥增加土壤速效 Ｎ的含量。土壤 ＮＯ_３~－的季节性变化表明 ５至 ６月份土壤中 ＮＯ_３~－的含量高,而花前和 ７月份土壤中 ＮＯ_３~－含量低。 Ｐ、Ｋ在清耕带 １０ ｃｍ以下土壤的含量低于生草地土壤,但在 ０～ １０ｃｍ土层中Ｋ的含量高于生草地。Ｐ的含量不受影响。施Ｎ减少了土壤Ｐ、Ｋ的含量。土壤中Ｍｇ的含量分布均匀,不受施Ｎ和地面管理制度的影响。     

槲皮素对苎麻、涤纶的吸附性能初步研究

本文以生物黄酮配基槲皮素为分散染料,对苎麻分别在77℃乙醇染浴中和在93℃水染浴中进行了染色实验,并与合成纤维涤纶进行对比,为苎麻和涤纶用天然染料进行染色的工艺选择提出了初步方案。结果表明槲皮素是一种理想的环保天然分散染料。     

水稻矮缩病毒对黑尾叶蝉卵巢发育与产卵量的影响

虫传植物病毒须依靠媒介昆虫进行传播和扩散,而植物病毒在媒介昆虫体内的复制和转运会对昆虫产生直接或间接影响.Sinisterra等[1]报道称媒介昆虫携带病毒后寿命缩短、产卵量下降.     

进口原木携带的截材小蠹属昆虫的检疫鉴定

本文介绍了我国口岸进口木材中多次截获的截材小蠹属(Truncaudum Hulcr and Cognato,2010)昆虫,对口岸常见该属具有重要经济和检疫意义的种类,提供重要形态特征描述和图片,并对该属所有种类进行简单介绍,包括重要鉴别特征、分布等信息,以期为口岸一线的检测鉴定提供参考。     

油菜菌核病菌对多菌灵和乙霉威的抗药性机理

生物测定结果表明,油菜菌核病菌田间菌株对多菌灵(MBC)敏感性表型呈多样性,即存在MBCS、MBCLRLR、MBCHRHR和MBCVHR表型.而对乙霉威(DIE)则只检测到DIES和DIEHR表型.MBCS、MBCLR和MBCHR菌株中除JD2-3菌株为DIES外,其余菌株均为DIEHR,MBCVHR菌株对DIE表现为DIES.MBC和DIE之间存在典型的负相关交互抗性.序列分析结果表明,表型为MBCVHRDIES菌株的β-微管蛋白基因,第198位氨基酸由Glu(GAG)突变为Ala(GCG);表型为MBCHRDIEHR菌株的β-微管蛋白基因的突变位点在第200位,由Phe(TTC)突变为Tyr(TAC);而表型为MBCSDIES和MBCLRDIEHR的菌株在所扩增的β-微管蛋白基因片段中未发生突变.初步表明,β-微管蛋白基因198和200位氨基酸的突变是引起油菜菌核病菌对多菌灵抗药性呈多样性的分子机理.