桃需冷量遗传特性的研究

对桃的９个杂交组合４６３株杂种实生苗需冷量进行测定,统计结果表明：桃需冷量是由多基因控制的,Ｆ＿１需冷量平均值较亲中值短,不同组合偏离程度不同,南山甜桃、红日、玛丽维拉、白凤、大久保需冷量育种值分别为１５９、２１６、３１９、８３１、８３４ｈ,遗传传递力分别为７９%、５４%、９２%、８３%、９３%。需冷量的遗传力为８９．６９%。萌芽开花与需冷量密切相关。     

干巴菌根际土壤理化性质的研究

本言语报道昆明小哨和宜良良竹山两地区两种植被类型和两种成土母质发育的土壤上，干巴菌只能在弱酸性的沉积岩类（石灰岩或砂页岩）或河流沉积物类成土母质发育形成的山原红壤的针叶林型的云南松或阔叶林型的麻栎林下生长发育。而其它成土母质形成的土壤中云南松林或麻栎林下，不会长出干巴菌。（２）干巴菌根际土壤必须具有良好的理化性质－－土壤疏松多孔、透水透气和土温传递优良；ＰＨ值在４．５－５．５区间内；土质富含钙和铁     

大肠杆菌耐热性肠毒素ST1—K99／LacZ基因重组的研究

将构建的ｐＢＳＴ２～６工程菌质粒用限制性内切酶ＢａｍＨＩ／ＢｇｌⅡ酶切，经琼脂糖凝胶电泳和电洗脱，回收１４７ｂｐ的目的ＳＴ１基因。随之将该基因分别重组到能有效表达Ｋ９９菌毛抗原和ＬａｃＺ酶的ｐＧＫ９９之Ｋ９９基因ＢｇｌⅡ位点和ｐＵＣ１８的ＢａｍＨＩ位点中。通过ＳＴ１基因探针菌落原位杂交、特定酶切分析及ＤＮＡ序列分析，筛选并鉴定出了理想重组子，从而构建出了能分别表达ＳＴ１融合基因产物的工程菌株ｐＳＫ２１９和ｐＸＳＴ１。     

溶剂法制歧化松香

将松脂或松香溶于溶剂中，Ｃ Ｐｄ／Ｃ为催化剂，≤２００℃反应，可得色泽浅与不皂化物含理低的歧化松香。对该歧化松香组成研究后表明，在上条件下除含有通常的少量脱羧产物外，尚有降解产物，它带羧基，分子量为２７６，没有其它小分子降解产物，因而收率较高，不皂化物少。     

恩肥对平菇菌丝生长,产量及品质的影响

本文报道了恩肥的施用浓度、施用时期对平菇菌丝生长、产量和品质的影响。试验结果表明,施用恩肥可促进平菇菌丝生长和子实体发育,有利于提高产量及改善品质。用肥量为每1000ml PDA或每公斤干培养料0.2～0.3ml时,菌丝生长速度显著加快,长满袋的时间可缩短4～5d,子实体产量可提高45.7％～55.9％,蛋白质、必需氨基酸和非必需氨基酸分别增加25.8％、14.94％和11.32％。在不同施肥时期中,又以现蕾期喷施效果最佳。     

青海绵羊血清铁传递蛋白的电泳研究

采用聚丙烯酰胺凝胶电泳法对青海省５个品种共１０５０只绵羊的血清铁传递蛋白进行了电泳研究。结果发现青海绵羊的血清运铁蛋白共有１１个电泳变异体（Ｉ，Ａ，Ｇ，Ｂ，Ｌ，Ｃ，Ｄ，Ｍ，Ｅ，Ｑ，），构成３７种电泳表型（ＩＡ，ＡＥ，ＡＡ，ＡＧ，ＡＢ，ＡＬ，ＡＣ，ＡＤ，ＡＭ，ＡＥ，ＡＢ，ＧＧ，ＧＢ，ＧＬ，ＧＣ，ＧＤ，ＧＭ，ＧＱ，ＢＢ，ＢＣ，ＣＤ，ＣＭ，ＬＣ，ＬＤ，ＬＥ，ＣＣ，ＣＤ，ＣＭ，ＣＥ，ＣＱ，ＣＰ，ＤＤ，ＤＭ，ＤＥＤＱ，ＫＰ，ＭＱ）。在５个品种绵羊中，藏羊血清铁传递蛋白的电泳变异体和电泳表型最多，分别为１１个和２８种，茨盖羊的最少，分别为６个和１６种，青海细毛羊，新疆细毛羊和青海半细胞毛羊介于两者之间。     

分散松香施胶剂性能影响因素的探讨

对逆转法制备分散松香施胶剂过程中的主要因素如乳化剂的种类及用量、马来松香加合物含量、搅拌转速、加料速度和乳化温度等系统地进行了探讨，提出了合理的工艺条件，并对实际生产中可能出现的问题及解决办法进行了阐述。     

Factors influencing decline in soil pH in Hawaiian Eucalyptus and Albizia plantations

Soil pH declined from 5.9 to 5.0 in 8 years beneath plantations of Eucalyptus saligna (Sm.) in Hawaii. In stands of Albizia falcataria, (L.) Fosberg, the soil pH change was more dramatic, declining from 5.9 to 4.6. We measured several components of soil acidity beneath four mixtures of the two tree species to gain insight on the processes responsible for the decline in soil pH. These components were studied using an empirical method of comparing acid quantity, degree of neutralization (depletion of base cations), and acid strength. The decline in soil pH differed between species as a result of differences in the degree of neutralization of the soil exchange complex; the larger decrease in soil pH under Albizia was produced by greater acidification of the exchange complex. Empirical titration curves suggested that differences in acid strength moderated the divergence in soil pH beneath the species. Had the acids accumulating in the soil under Albizia been as strong as those in the Eucalyptus soil, the difference in soil pH would have been greater. Though the two species had contrasting effects on soil pH, the differences in degree of neutralization, responsible for the pH decline, were small compared with differences in the amount of cations stored in tree biomass. Continued supply of nutrient cations (from weathering or fertilization) will ultimately control both the extent of soil pH decline and the level of productivity sustained by the forest.     

青海湟水流域水资源及水环境

目前，湟水流域水量不足，水质污染，已成为该流域经济发展的制约因素。为此，防治湟水流域水污染，保护水资源，改善水环境，维护流域生态平衡，是我省经济和社会发展中的一项刻不容缓的紧迫任务。     

Monoclonal antibodies to bovine viral diarrhea virus: cross-reactivities to field isolates and hog cholera virus strains.

Monoclonal antibodies to bovine viral diarrhea virus (BVDV) were examined for binding with a large number of North American BVDV isolates and eight strains of the serologically related pestivirus, hog cholera virus (HCV). No single BVDV monoclonal antibody reacted with all BVDV isolates. The most cross-reactive monoclonal antibody was an anti-p80/p125 antibody which showed a positive reaction with 173 of 180 (96%) North American isolates. From a fewer number of isolates tested, one anti-gp53 monoclonal antibody also showed a high cross-reactivity (94%). All BVDV isolates showed a positive reaction with at least one of the seven monoclonal antibodies in the panel. Thus, the results indicated that a pool of these monoclonal antibodies may be used in place of polyclonal antisera for the detection of BVDV contamination of cell lines or for virus isolation. For HCV, all three anti-p80/p125 monoclonal antibodies reacted positively with all eight virus strains. In contrast, none of the anti-gp53 monoclonal antibodies were reactive to HCV strains. Thus, the anti-gp53 monoclonal antibodies may be useful for distinguishing between usually innocuous BVDV infections and the highly significant HCV infections in swine for foreign animal disease surveillance.