水稻耐盐机理的研究Ⅲ.不同基因型对NaCl吸收和运转的动力学比较

本文应用动力学研究方法比较了具有不同耐盐性的3组共6个水稻基因型对NaCl吸收和运转的差异,结果表明,在外部低NaCl浓度(0.1mmolL~(-1)时,水稻对Na~+的吸收为被动吸收,耐盐基因型对Na~+的吸收速率显著低于盐敏感基因型;对Cl~-的吸收为主动吸收,不同耐盐性基因型吸收Cl~-的动力学参数(V_(?)、K_(?))差异不显著,在外部高NaCl浓度(50mmolL~(-1))时,耐盐基因型对Na~+和cl~-的吸收速率均低于对应的盐敏感基因型。耐盐基因型水稻的Na~+、cl-从根部向地上部的运转率低于对应的盐敏感基因型,这种差异在高盐浓度时更为明显。表明了耐盐基因型水稻地上部对NaCl的排斥作用是吸收控制和运转控制共同作用的结果,使地上部Na~+、Cl-含量相对降低而显出较高的耐盐性。     

俄罗斯主要采脂树种——欧洲赤松松脂的化学组成

德国Ｗ．Ｌａｎｇｅ在研究不同地区来源的欧洲赤松松脂的化学组成时，曾按△~３－蒈烯含量不同，将它们分成中欧和南欧地区二类。本文研究俄罗斯境内的欧洲赤松松脂的组成，发现它与同纬度的中欧地区欧洲赤松松脂一样，含有较高数量的△~３－蒈烯。同时发现，在中国境内的欧洲赤松的２个变种──樟子松和长白松松脂中△~３－蒈烯含量也随纬度不同而有明显差别。     

家兔选育性状典型相关分析

选择日本大耳白和新西兰两个品种家兔的生长、繁殖、以及体尺3组共17个性状，应用多元统计方法进行了典型相关分析，结果表明；日本大耳白兔的初生重、初生窝重、21日龄重、泌乳力、胸围等5个性状，新西兰兔的产活仔数、初生重、21日龄重、泌乳力、断奶窝重、母兔体重等6个性状为主要性状。     

重视粪污处理 改善养殖环境

近20多年来，我国养殖业发展迅速，取得了可喜的业绩。我国肉类、蛋类总产量连续多年保持世界第一，养殖业为改善我国人民生活水平，调整人们膳食结构、提高农民收入做出了巨大的贡献。但它的发展不可避免地产生了大量的“畜产公害”，畜禽粪便、养殖污水任意堆弃、排放现象普遍存在。     

桉树的一个国内新记录病原菌

本文报道了寄生在雷林一号桉“[Eucalyptus leichou NO:1]”叶片上的一个国内新记录病原菌[Fairmaniella leprosa(Fairman)Petrak & Sydow.]的主要特征,并与已知种作了比较;文中讨论了国内外研究状况,寄主及分布。标本采集于广东省雷州林业局林科所人工桉树林。     

Biological characteristics of long passaging rat mesenchymal stem cells

AIM:To investigate multi-potential of rat bone marrow mesenchymal stem cells (rBMMSC) and mutation inclination, the rBMMSC were long passaged in vitro. METHODS:Cellular cycles of different passages were assayed by FACSan flow cytometry and karyotypes of passage 6, passage 25 and passage 45 were compared by G-binding analysis. RESULTS:The early passages and long-term passages all showed strong proliferation; passage 6, passage 25 and passage 45 all showed normal karyotype. CONCLUSION:Long-term culture and passage of rBMMSC still remains strong proliferation. With this capability, the mutation inclination is not enhanced.     

Preparation of gfp-bcl-XL-contained recombinant adenovirus vector by the homologous recombination in bacteria

AIM: To prepare gfp-bcl-X_L-contained recombinant adenovirus(rAd-gfp-bcl-X_L).METHODS: Bcl-X_L gene was amplified from pEGFP-C₃-bcl-X_L, subcloned into shuttle plasmid and formed transfer plasmid of pAdTrack-CMV-bcl-X_L. Then pAdTrack-CMV-bcl-X_L was linealinzed with PmeI and co-transformed into BJ5183 bacteria with adenovirus genomic plasmid of pAdEasy-1. The identified recombinant adenovirus plasmid was digested with PacI and transfected into 293 cells to package recombinant adenovirus particles. The target gene was detected by PCR.RESULTS: There were about 35% positive recombinant bacterial clones after the co-transformation of pAdTrack-CMV-bcl-X_L and pAdEasy-1 into BJ5183. Recombinant adenovirus particle were produced and further amplified after the transfection of pAdEasy-1-gfp-bcl-X_L into 293 cells. PCR test indicated that the recombinant Ad contained bcl-X_L gene. The titer of the purified rAd-gfp-bcl-X_L was 6.5×10¹² PFU/L. CONCLUSIONS: The homologous recombination in bacteria is a convenient and high efficient method to prepare rAd-gfp-bcl-X_L. This affords a good gene transfer vector for the gene therapy in human’s diseases.     

Effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts

AIM: To investigate the effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts.METHODS: hMSC were separated from human marrow and expanded in cuture medium. hMSC were induced with dexamethasone, β-glycerophosphate, vitamin C which acted as osteoblast differentiation inducer. PD98059 was added into the osteoblasts induction medium. The cells were assayed with cell morphology, alkaline phosphatase (AP) activity and calcium deposition. RESULTS: The isolated cultured MSC comprised a single phenotypic population and displayed a fibroblast-like morphology. After induced with osteoblasts induction medium, the cells showed changes in cell morphology from spindle-shape to cuboidal and polygonal. The AP activity increased gradually and reached the peak in 12 days, then decreased. Many scattered tangerice calcium nodes were observed. PD 98059 significantly inhibited AP activity and calcium deposition in a dose-dependent manner. A striking observation of the present study was that a few adipocytes appeared in cultures that were treated with PD 98059 and osteogenic differentiation medium. CONCLUSION: These results indicated that osteogenic diferentiation from the hMSCs was related to the activation of the ERK.     

喀喇昆仑山盖孜河水文水资源特性分析

喀喇昆仑山河流自有水文资料记载以来 ,近 10年洪灾发生频率明显高于以往 ,人民的生命财产损失程度也大幅度增加。在分析了盖孜河流域的径流及气候多年变化过程后 ,认为洪灾频发的原因是近年来流域内降水及气温呈上升趋势 ,导致高山冰川退缩 ,河流径流增加 ,洪灾增大、频率增高 ;小区域多年气候变化过程从侧面印证了我国西北地区气候由暖干向暖湿变化。     

苹果梨的育种价值

该文指出,苹果梨综合性状优良,是很好的梨育种种质资源;以苹果梨及其后代为亲本已选育出30余个新品种,大大丰富了梨种质资源;苹果梨的大果、耐贮、小果心、抗寒等优良性状在后代品种中有较强的表现;以苹果梨与多倍体品种杂交后代为多倍体的比率较高。并展望了苹果梨在今后育种中的主要研究方向。