全文获取类型
收费全文 | 604篇 |
免费 | 14篇 |
专业分类
林业 | 26篇 |
农学 | 47篇 |
基础科学 | 4篇 |
67篇 | |
综合类 | 14篇 |
农作物 | 25篇 |
水产渔业 | 9篇 |
畜牧兽医 | 399篇 |
园艺 | 8篇 |
植物保护 | 19篇 |
出版年
2023年 | 5篇 |
2022年 | 5篇 |
2021年 | 9篇 |
2020年 | 9篇 |
2019年 | 17篇 |
2018年 | 19篇 |
2017年 | 10篇 |
2016年 | 11篇 |
2015年 | 7篇 |
2014年 | 14篇 |
2013年 | 79篇 |
2012年 | 18篇 |
2011年 | 21篇 |
2010年 | 14篇 |
2009年 | 15篇 |
2008年 | 12篇 |
2007年 | 16篇 |
2006年 | 7篇 |
2005年 | 5篇 |
2004年 | 8篇 |
2003年 | 7篇 |
2002年 | 4篇 |
2001年 | 9篇 |
2000年 | 6篇 |
1999年 | 5篇 |
1998年 | 7篇 |
1997年 | 5篇 |
1996年 | 4篇 |
1995年 | 4篇 |
1994年 | 6篇 |
1993年 | 5篇 |
1989年 | 6篇 |
1988年 | 4篇 |
1976年 | 7篇 |
1968年 | 14篇 |
1966年 | 6篇 |
1964年 | 7篇 |
1963年 | 7篇 |
1960年 | 26篇 |
1959年 | 10篇 |
1957年 | 21篇 |
1956年 | 12篇 |
1955年 | 26篇 |
1954年 | 23篇 |
1944年 | 10篇 |
1943年 | 5篇 |
1942年 | 6篇 |
1941年 | 19篇 |
1940年 | 6篇 |
1927年 | 5篇 |
排序方式: 共有618条查询结果,搜索用时 154 毫秒
91.
92.
93.
94.
Expression and localization of angiopoietin family in corpus luteum during different stages of oestrous cycle and modulatory role of angiopoietins on steroidogenesis,angiogenesis and survivability of cultured buffalo luteal cells 下载免费PDF全文
SR Mishra MS Parmar VP Yadav R Reshma J Bharati MK Bharti A Paul VS Chouhan G Taru Sharma G Singh M Sarkar 《Reproduction in domestic animals》2016,51(6):855-869
The objective of this study was to document the expression and localization of angiopoietin (ANGPT) family members comprising of angiopoietin (ANGPT1 and ANGPT2), and their receptors (Tie1 and Tie2) in buffalo corpus luteum (CL) obtained from different stages of the oestrous cycle, and the modulatory role of ANGPT1 and ANGPT2 alone or in combinations on progesterone (P4) secretion and mRNA expression of phosphotidylinositide‐3kinase‐protein kinase B (PI3K‐AKT), phosphoinositide‐dependent kinase (PDK), protein kinase B (AKT), Bcl2 associated death promoter (BAD), caspase 3 and von willebrand factor (vWF) in luteal cells obtained from midluteal phase (MLP) of oestrous cycle in buffalo. Real‐time RT‐PCR (qPCR), Western blot and immunohistochemistry were applied to investigate mRNA expression, protein expression and localization of examined factors whereas, the P4 secretion was assessed by RIA. The mRNA and protein expression of ANGPT1 and Tie2 was maximum (p < .05) in mid luteal phase (MLP) of oestrous cycle. The ANGPT2 mRNA and protein expression was maximum (p < .05) in early luteal phase, decreased in MLP and again increased in late luteal phase of oestrous cycle. ANGPT family members were localized in luteal cells and endothelial cells with a stage specific immunoreactivity. P4 secretion was highest (p < .05) with 100 ng/ml at 72 hr when luteal cells were treated with either protein alone. The mRNA expression of PDK, AKT and vWF was highest (p < .05) and BAD along with caspase 3 were lowest (p < .05) at 100 ng/ml at 72 hr of incubation period, when cultured luteal cells were treated with either protein alone or in combination. To conclude, our study explores the steroidogenic potential of angiopoietins to promote P4 secretion, luteal cell survival and angiogenesis through an autocrine and paracrine actions in buffalo CL. 相似文献
95.
We report the results of investigations that were conducted in a sheep flock in Uttaranchal, India where repeated failure
of anthelmintic medication was noted. The study revealed that Haemonchus contortus in sheep had developed resistance to benzimidazoles (fenbendazole, mebendazole and albendazole), imidazothiazole (levamisole)
and salicylanide (rafoxanide), while it was fully susceptible to avermectins (ivermectin). Further, the suppression of nematode
egg output in faeces of sheep naturally infected with multiple anthelmintic-resistant H. contortus following treatment with ivermectin tablet (0.4 mg/kg body weight (bw), orally), ivermectin injection (1% w/v, 0.2 mg/kg
bw, subcutaneously) and ivermectin pour-on (0.5 w/v, 0.5 mg/kg bw) was also studied over a period of 10 weeks post treatment.
It was noted that ivermectin tablet after initial clearance of infection (faecal egg count reduction 100%), could not prevent
establishment of new patent natural infection for even a single day, while ivermectin pour-on and injection prevented the
establishment of new infection for 7 and 14 days post treatment, respectively. Maximum protection period (duration for which
mean faecal egg count of sheep reaches 500 eggs per gram of faeces or more) of 68 days was recorded in sheep treated with
injectable ivermectin, followed by pour-on (60 days) and oral (53 days) preparations. 相似文献
96.
A study was conducted to demonstrate the comparative efficiency of acid phosphatase generated by plants or fungi towards the hydrolysis of different organic P compounds present in soil. The results revealed that acid phosphatases were most efficient in the hydrolysis of glycerophosphate followed by lecithin and phytin. The P release increased with increase in enzyme concentration. Acid phosphatase generated from fungal sources showed three times greater efficiency in the hydrolysis of phytin, two times greater efficiency in hydrolysis of lecithin than plant phosphatase. Both sources were at par in hydrolyzing glycerophosphate. The results suggest that acid phosphatase generated from plant and fungal sources is different and microbial acid phosphatase to be more efficient than that from plant sources. 相似文献
97.
Seunghan Lee Vikas Kumar Beth Cleveland Nicholas Romano Goutham N Vemuri Amit K. Yadav Kristen Meiler Ronald W. Hardy 《Aquaculture Research》2020,51(10):4065-4074
The rapid global growth in fish farming and limited supply of fish meal (FM) has consequently reduced FM inclusion levels in compound feeds leading to a higher reliance on alternative protein sources. Sasya is a single cell protein (SCP) product that has a similar amino acid profile as FM. The aim of this study was to evaluate the effects of replacing FM with SCP on in vivo digestibility, growth, feed efficiency, whole‐body proximate/amino acid composition and gene expression levels of various hepatic enzymes in rainbow trout (Oncorhynchus mykiss). Three isonitrogenous (470 g/kg crude protein) and isolipidic (18 g/kg crude lipid) diets were formulated as follows: Diet 1: control (30% FM); Diet 2:24% FM + 6% SCP and Diet 3:18% FM + 12% SCP. Each diet was hand‐fed to triplicate tanks containing 30 rainbow trout fingerlings (4.99 ± 0.20 g) for 9 weeks. Apparent digestibility coefficients of SCP for dry matter, crude protein, lipid and energy were 60, 80, 93 and 74% respectively. Growth performance (final weight: 69–71 g), feed conversion ratios (0.91–0.94) as well as whole‐body protein and amino acid composition were unaffected by diets. However, Diet 3 significantly increased whole‐body crude fat and energy. Fish fed the SCP‐based diets had significantly higher expression for carnitine palmitoyltransferase‐1b (CPT1b), fatty acid delta 6 desaturase (FADS6) and fatty acid elongase 5 compared to the control. Overall, the quality of the SCP was similar as FM. Therefore, this product could enlarge the portfolio of alternative protein sources that can be used in fish diets and thus open a new market opportunity for use of a new feed resource in the feed industry. 相似文献
98.
A comparison of intra‐ and extracellular acid phosphatase, alkaline phosphatase and phytase activity in six fungi is reported. A strong linear relationship between intra versus extracellular fungal acid phosphatase (R2 = 0.94), alkaline phosphatase (R2 = 0.96), and phytase (R2 = 0.97) is observed. Three‐fourth of acid phosphatase were generally present inside the fungal cells and only 25 % were released extracellularly after a three weeks period. Phytase shows the reverse trend where thirty nine times higher extracellular phytase activity was noticed than present inside the fungal cells. The extracellular enzymes are found 60 % more efficient in the hydrolysis of phytin than their intracellular counterpart but they are at par in the hydrolysis of glycerophosphate. The results clearly demonstrated that phytase types of phosphatases mostly occur outside the fungal cells whereas most of the acid phosphatase and alkaline phosphatase are located inside the cells.<?show $6#> 相似文献
99.
100.
Field experiments were carried out during rainy (kharif) and winter (rabi) seasons (June–April) of 2008–2010 at Indian Agricultural Research Institute (IARI), New Delhi, to study the productivity, nutrients uptake, iron (Fe) use-efficiency and economics of aerobic rice-wheat cropping system as influenced by mulching and Fe nutrition. The highest yield attributes, grain and straw yields (5.41 tonnes ha?1 and 6.56 tonnes ha?1, respectively) and nutrient uptake in rice was recorded with transplanted and puddled rice (TPR) followed by aerobic rice with Sesbania aculeata mulch. However, residual effect of aerobic rice with wheat straw mulch was more pronounced on yield attributes, grain and straw yields (4.20 and 6.70 tonnes ha?1, respectively) and nutrient uptake in succeeding wheat and remained at par with aerobic rice with Sesbania mulch. Application of iron sulfate (FeSO4) at 50 kg ha?1 + 2 foliar sprays of 2% FeSO4 was found to be the best in terms of all the yield attributes, grain and straw yield (5.09 and 6.17 tonnes ha?1, respectively) and nutrient uptake and remained at par with 3 foliar sprays of 2% FeSO4. Although residual effect of iron application failed to increase the yield attributes, yield and nutrient uptake nitrogen, phosphorus and potassium (N, P, K) except Fe. The highest system productivity, nutrient uptake, gross returns, net returns, B: C ratio and lowest cost of cultivation were recorded with aerobic rice with wheat straw and Sesbania aculeata mulch. Application of FeSO4 at 50 kg ha?1 + two foliar sprays of 2% FeSO4 was found better in respect of system productivity, nutrient uptake, gross returns, net returns, B:C ratio and cost of cultivation in aerobic rice-wheat cropping system. The Fe use efficiency values viz. partial factor productivity (kg grain kg?1 Fe), agronomic efficiency (kg grain increased kg?1 Fe applied), agrophysiological efficiency (kg grain kg?1 Fe uptake), physiological efficiency (kg biomass kg?1 Fe uptake), apparent recovery (%) utilization efficiency and harvest index (%) of applied Fe were significantly affected due to methods of rice production and various Fe nutrition treatments in aerobic rice and aerobic rice-wheat cropping system. 相似文献