Maximizing Sugarcane Yield by Increasing Plant Population Density, Minimizing NO3-N Leaching and Improving Soil Organic Matter in Different Crop Rotations

In a field experiment conducted during 1992–95 at Lucknow, India, sugarcane was planted in rows 60 and 90 cm apart in three crop rotations (rice-sugarcane-ratoon, Sesbania aculeata for green manure-sugarcane-ratoon, and cowpea-sugarcane-ratoon) with 0, 150 and 300 kg N ha⁻¹ as urea either with or without farmyard manure (FYM) at 10 t ha⁻¹. Sugarcane yields were significantly greater in the Sesbania rotation than in the other because of a larger N uptake. N uptake of the crop was significantly affected by soil organic carbon, and available N and K contents. Ratoon yields, however, were largest in the cowpea sequence followed by the rice rotation, probably due to a prolonged residual effect of cowpea and rice root residues. The residual effect of a Sesbania green manure was negligible as demonstrated by the low NO₃-N content of the soil profile after sugarcane harvest compared to the other two crop sequences. The total cane productivity (main sugarcane plus ratoon) was greater (156 t ha⁻¹) in the cowpea rotation than the Sesbania (152 t ha⁻¹) and rice (140 t ha⁻¹) rotations.     

Development of a method for the identification, using the polymerase chain reaction, of Aspergillus fumigatus isolated from ostriches

Objective To develop a method for identifying DNA of Aspergillus fumigatus from ostriches, using the polymerase chain reaction (PCR). A fumigatus is the principal causative agent of avian aspergillosis.
Design A biochemical trial.
Sample population Twelve Aspergillus fumigatus isolates and three other Aspergillus species.
Procedure PCR primers that were based on the sequence of the alkaline protease gene from human isolates of A fumigatus were used.
Results We successfully tested the method on ostrich isolates from five states and showed that the test is specific for A fumigatus.
Conclusions In most cases the DNA sequence of A fumigatus isolates from ostriches is similar to that of human isolates. DNA sequences vary significantly among A fumigatus isolates, including those from affected ostriches in the same flock. The genetic variation may be used to trace aspergillus infections in ostrich flocks and determine if the disease is transmitted by contact with infected birds.