极小种群野生植物八蕊单室茱萸的种群状况研究

八蕊单室茱萸为云南省特有的极小种群野生植物和濒危物种。通过查阅相关文献并结合访问调查,确定八蕊单室茱萸可能分布的区域,开展种群资源调查。结果显示:八蕊单室茱萸目前仅在普洱市和西双版纳州有10个天然种群,其种群规模已低于最小可存活种群,属极小种群野生植物和濒危物种,亟需开展保护工作。生境破坏和生境片段化是导致八蕊单室茱单室茱萸濒危的人为因素。提出收集种质资源、营建和管护近地保护种群、建立迁地和回归种群等保育建议。     

马链球菌兽疫亚种烯醇化酶对小鼠肺泡巨噬细胞吞噬功能的影响

旨在研究马链球菌兽疫亚种（Streptococcus equi ssp.zooepidemicus，SEZ）烯醇化酶（enolase，Eno）对小鼠肺泡巨噬细胞（RAW264.7）吞噬能力的影响。通过构建原核表达质粒获得重组烯醇化酶（rEno），采用台盼蓝活细胞计数法，判定在不同处理浓度和时间下rEno蛋白对RAW264.7细胞的细胞毒性。将rEno蛋白与RAW264.7细胞共孵育后，用SEZ作用于细胞并检测细胞吞菌数量，判断RAW264.7细胞对SEZ的吞噬活性。进一步通过活细胞稳定同位素标记技术（SILAC）和蛋白质谱分析技术（LC-MS/MS），筛选到RAW264.7细胞中可能与SEZ Eno存在相互作用的候选蛋白。结果发现，10 μg·mL^-1 rEno蛋白处理对RAW264.7细胞有明显的细胞毒性，且10 μg·mL^-1 rEno蛋白处理RAW264.7细胞2和4 h可显著抑制其对SEZ的吞噬作用（P<0.01、P<0.05）。初步筛选到RAW264.7细胞中动力蛋白激活蛋白亚单位蛋白（dynactin subunit protein 2，Dctn）、整合素α-M蛋白（integrin alpha-M）等17种可能与Eno发生互作的蛋白。本研究获得了rEno重组表达蛋白，发现rEno可减少RAW264.7细胞对SEZ的吞噬，互作蛋白的初步筛选也为进一步揭示Eno在SEZ抗吞噬中的作用机制奠定了基础。     

Whistle signal variations among three Indo-Pacific humpback dolphin populations in the South China Sea: a combined effect of the Qiongzhou Strait's geographical barrier function and local ambient noise?

Geographic variations in the dolphin whistles could be useful in assessing association and isolation among populations. Whistle of free-ranging Indo-Pacific humpback dolphins (Sousa chinensis) among the Pearl River Estuary (PRE), Leizhou Bei (LZB) and Sanniang Bay (SNB) populations were investigated. A total of 2850 whistles with legible fundamental contour were extracted and 15 acoustic parameters were measured. Contrary to SNB, PRE and LZB had the same relative proportion of tonal type compositions with flat and sine representing the most frequent types. The generalized linear model analysis showed significant acoustic difference among populations and tonal types. All frequency parameters in SNB were significantly higher than those in PRE and LZB, where no significant variation was observed in most of the parameters either at the population level or within each tonal type. Canonical discriminant functions analysis showed a smaller difference between PRE and LZB than between PRE and SNB and between LZB and SNB. Compared with previous recordings, recent recordings demonstrated a consistent pattern of becoming higher in whistle frequency parameters in both LZB and SNB populations, suggesting that noise pollution in LZB and SNB increasing with time according to the acoustic niche hypothesis. Dolphin whistle's geographic variations could be shaped by the combined function of the geographical barrier function of the Qiongzhou strait and local ambient noise. Considering the isolated condition and the relatively smaller population size of the humpback dolphin in the SNB, more effective and proactive conservation actions should be taken to prevent the extinction of small populations.     

春白萝卜新品种雪单3 号的选育

雪单3 号是利用双单倍体育种技术选育的春白萝卜新品种。母本ED0108A 是以梅花春萝卜× 白玉春萝卜进行花药培
养获得的DH 系为轮回父本，与不育系进行多次回交，转育成的雄性不育系。父本ED3128 是由迟花春萝卜× 天鸿春萝卜进行
花药培养获得的DH 系。该品种生育期60 d（天），植株开展度45 cm，裂叶，小叶11 对，叶片数20，叶簇平展，叶色深绿；
肉质根长圆柱形，白皮白肉，根长36 cm，横径7.1 cm，单根质量1.1 kg，高抗黑腐病、芜菁花叶病毒病、黄瓜花叶病毒病和花
椰菜花叶病毒病，抗霜霉病，群体整齐度高。每667 m² 产量4 000～5 000 kg。适宜在湖北、湖南、河南、山东等地区早春栽培。     

秦岭南麓新分布小太平鸟迁徙期食性初步观察

2011-2013年冬季,作者采用分组全天跟踪的方法,调查小太平鸟迁徙期活动特征和食性。结果证明:小太平鸟冬季由北向南成群迁徙过程中,会在某个生境优良的区域停留取食9~15日、成群活动在树冠上部。小太平鸟在迁徙期停留区域取食植物果实种类有20种,有人工栽培树种果实,也有野生树种果实。今后应持续迁移路线、停歇地、种群动态监测。     

Establishment and Optimization of INH Epitope Peptide Vaccine for Detection of Antibody

In this study, an indirect ELISA method was established to detect inhibin hormone (INH) epitope peptide vaccine antibody, it would provide oretical reference for the determination of Fine-wool sheep after active immune body INH epitope peptide vaccine antibody. On the basis of the predecessors, using indirect ELISA method to determinate serum INH epitope peptide antibody levels of sheep, and through control different experimental conditions to look for the best experimental conditions. Through explorating the experimental conditions, finally, the testing experiment conditions were determined, which was blocked solution with skimmed milk powder, INH and GnIH synthetic peptides dilution degrees for 20 000 times, the optimum reaction time was 60 min, the best color action time was 15 min. In this experiment, a kind of method to detection antibody in the body after INH active immune sheep was built, it would provide a reference for future research.     

基于熵算法的股票指数高频数据复杂度测算与评价

在日内高频环境下检验基于兼容法的柯尔莫哥洛夫熵、样本熵和模糊熵等复杂度测算方法对我国沪深300股票指数的测算效率，并运用筛选后的有效算法分阶段研究和比较了序列复杂度的变化过程与变化幅度.结果表明，模糊熵算法是一种更适用于我国沪深300股票指数的有效复杂度测算方法，其对相似容忍度的敏感性更低，测度值连续性更好.随时间推移，我国沪深300股票指数复杂度整体呈上升趋势,而相较于发达市场甚至周边新兴市场其复杂度偏低.     

鸡胚及禽类细胞系在生物医药领域的应用及研究进展

鸡胚因发育过程清楚，长久以来作为基础和应用科学研究领域重要的实验模型，尤其在鸡胚发育早期绒毛尿囊膜阶段，因其血管丰富，是天然的免疫缺陷宿主，是病理学、药理学和肿瘤学等研究领域的理想实验模型。作者简述了发育早期的鸡胚组织结构，并介绍了鸡胚绒毛尿囊膜在肿瘤研究、血管生成、器官移植、烧伤等疾病机理研究中的应用，以及在鸡胚病理模型基础上进行的抗肿瘤药物筛选的应用。重点介绍了鸡胚及禽类细胞系在病毒繁殖和疫苗生产、治疗性蛋白和单抗生产方面的应用研究进展。多种人源病毒、禽源病毒、支原体等可在鸡胚及禽类细胞上增殖，并用于疫苗生产。作者对常用的禽类纤维原细胞和多能干细胞的发展和特点进行了阐述，并总结了商业化的禽类细胞系来源以及部分易感病毒。鸡胚表达系统能够在目的蛋白特定位点产生人源化糖基，减少目的蛋白对人的过敏反应，且禽蛋廉价易得，可作为生产人用单克隆抗体和治疗性蛋白的合适供体。作者介绍了鸡胚及禽类细胞系在生物医药领域应用的最新进展，并对鸡胚作为动物模型在未来的应用进行了展望。