留叶数对烟叶综合性状的影响研究

采用多因子裂区试验设计,研究留叶数对K326、HY-9-7综合性状的影响。结果表明:在相同的栽培条件下,品种HY-9-7的长势、抗病性均强于K326,产量、产值高于K326,外观质量优于K326。在中等肥力条件下,品种K326的适宜留叶数为20~22片/株,HY-9-7为18~20片/株。     

广昌县人工湿地污水处理模型

在环境和能源问题日益突出的时代背景下,将人工湿地应用于生活污水处理是当前十分推崇的新型污水处理方法,根据广昌独特的自然、社会、经济条件,设计相适应的人工湿地模型及植物配置,介绍人工湿地模型参数和运行过程中的技术问题及控制技术,为小城镇生活污水处理提供参考。     

Electroacupuncture-induced neural activation detected by use of manganese-enhanced functional magnetic resonance imaging in rabbits

OBJECTIVE: To investigate the effects of acupuncture on neural activity detected by use of manganese-enhanced functional magnetic resonance imaging (fMRI) and elucidate the relationship between somatic acupoint stimulation and brain activation. ANIMALS: 40 New Zealand White rabbits. PROCEDURE: Manganese-enhanced fMRI was performed in anesthetized rabbits manipulated with electroacupuncture (EA) on Zusanli (ST-36) and Yanglingquan (GB-34) acupoints. Image acquisition was performed on a 1.5T superconductive clinical scanner with a circular polarized extremity coil. T1-weighted images were acquired sequentially as follows: baseline, after mannitol injection, after manganese infusion, and 5 and 20 minutes after initiation of EA. RESULTS: Changes in focal neural activity were detected by use of manganese-enhanced fMRI. Stimulation on Zusanli (ST-36) for 5 minutes resulted in activation of the hippocampus, whereas stimulation on Yanglingquan (GB-34) resulted in activation of the hypothalamus, insula, and motor cortex. Activation became less specific after 20 minutes of EA. Furthermore, stimulation on ipsilateral acupoints led to bilateral brain activation. CONCLUSIONS AND CLINICAL RELEVANCE: Each acupoint has a corresponding cerebral linkage, and stimulation on these points resulted in time-dependent neural activation. Understanding the linkage between peripheral acupoint stimulation and central neural pathways may provide a useful guide for clinical applications of acupuncture.     

Anatid herpesvirus 1 CH virulent strain induces syncytium and apoptosis in duck embryo fibroblast cultures

Anatid herpesvirus 1 (AHV-1) CH virulent strain was first isolated from an infected duck and it was found that this virus strain could induce cytopathic effect (CPE) in duck embryo fibroblast (DEF). Following AHV-1 infection, DEF showed morphological changes such as cell rounding, improved refractivity and detachment from the culture surface. However, its pathological characteristics were not adequately known. Related studies were performed and the results showed that syncytium formation could be observed as the other type of CPE in AHV-1 infection. Hematoxylin-eosin staining and 4’, 6-diamidino-2-phenylindole (DAPI) staining of infected DEF were each used to visualize the shape and distribution of chromatin within nuclei and nuclear fragmentation was observed. Chromatin condensation and margination, as well as formation of apoptotic bodies were observed by transmission electron microscopy (TEM). DNA ladder formation was detected in AHV-1 infected cells and apoptosis of the infected DEF was also detected by flow cytometry analysis of Annexin V-FITC/PI staining method. Therefore, it was suggested that AHV-1 virulent strain can induce syncytium and apoptosis in DEF. Syncytium formation and apoptosis observed in this study may contribute to the elucidation of AHV-1 pathogenesis.     

不同土壤类型对膏桐幼苗耐高温性的影响研究

以膏桐实生盆栽幼苗为试验材料,研究其在不同土壤类型营养配方处理条件下水分饱和亏缺、质膜相对透性、丙二醛含量等的变化.结果表明:不同营养配方在25℃和35℃条件下,膏桐幼苗生长正常;在45℃高温处理下,随着高温时间的延长,燥红壤配方与赤红壤配方和砖红壤配方相比,叶片相对含水量下降幅度和质膜相对透性、丙二醛含量增高幅度较小,变化趋势较平缓.方差分析表明,燥红壤配方与赤红壤配方及砖红壤配方在高温期间的生理指标差异极显著,而赤红壤配方与砖红壤配方差异不显著.综合3种配方在高温期间的生理指标变化情况,其耐高温能力由强到弱依次为:燥红壤、赤红壤、砖红壤.     

栗疫菌弱毒力特征的传递及其影响因素

栗疫菌 (Cryphonectriaparasitica)弱毒力 (hypovirulence)的本质是菌体中含有一种弱毒性病毒 (cryphonectriahypovirulenceviruses ,CHV)dsRNA(Choietal,1992 ;Morrisetal,1979)。栗疫菌的弱毒力菌株 (H菌株 )一般都具有将强毒力 (virulent)菌株 (V菌株 )转化为弱毒力菌株的能     

Dissecting Genetic Basis of Deep Rooting in Dongxiang Wild Rice

Deep rooting is an important trait in rice drought resistance. Genetic resources of deep-rooting varieties are valuable in breeding of water-saving and drought-resistant rice. In the present study, 234 BC₂F₇ backcross introgression lines were derived from a cross of Dongye 80 (an accession of Dongxiang wild rice as the donor parent) and R974 (an indica restorer line as the recurrent parent). A genetic linkage map containing 1 977 bin markers was constructed by ddRADSeq for QTL analysis. Thirty-one QTLs for four root traits (the number of deep roots, the number of shallow roots, the total number of deep roots and the ratio of deep roots) were assessed on six rice chromosomes in two environments (2020 Shanghai and 2021 Hainan). Two of the QTLs, qDR5.1 and qTR5.2, were located on chromosome 5 in a 70-kb interval. They were detected in both environments. qDR5.1 explained 13.35% of the phenotypic variance in 2020 Shanghai and 12.01% of the phenotypic variance in 2021 Hainan. qTR5.2 accounted for 10.88% and 10.93% of the phenotypic variance, respectively. One QTL (qRDR2.2) for the ratio of deep roots was detected on chromosome 2 in a 210-kb interval and accounted for 6.72% of the phenotypic variance in 2020. The positive effects of these three QTLs were all from Dongxiang wild rice. Furthermore, nine and four putative candidate genes were identified in qRDR2.2 and qDR5.1/qTR5.2, respectively. These findings added to our knowledge of the genetic control of root traits in rice. In addition, this study will facilitate the future isolation of candidate genes of the deep-rooting trait and the utilization of Dongxiang wild rice in the improvement of rice drought resistance.     

胡萝卜黄色突变体的遗传及表现研究

 从胡萝卜‘新黑田五寸’品种的高世代自交系中发现了能稳定遗传的黄色突变体。遗传分析证明该黄色突变性状为单基因隐性遗传, 绿色对黄色为完全显性。黄色突变体叶片中叶绿素含量仅为正常株的29.3% ～50.1% , 且黄色不受温度光照等环境的影响。黄色突变体( yelyel) 地上部和地下部的生长量明显少于正常株系( YELYEL ) , 但在等位基因杂合时( YELyel) 与正常株系的生长量、产量和营养品质无显著差异。     

Laser altimetry of small-scale features on 433 Eros from NEAR-Shoemaker

During the Near Earth Asteroid Rendezvous (NEAR)-Shoemaker's low-altitude flyover of asteroid 433 Eros, observations by the NEAR Laser Rangefinder (NLR) have helped to characterize small-scale surface features. On scales from meters to hundreds of meters, the surface has a fractal structure with roughness dominated by blocks, structural features, and walls of small craters. This fractal structure suggests that a single process, possibly impacts, dominates surface morphology on these scales.     

牦牛铜锌超氧化物歧化酶原核表达载体的构建及其表达研究

【目的】为解决医用超氧化物歧化酶(superoxide dismutase,SOD)来源的限制,利用基因工程方法构建牦牛Cu,Zn-SOD原核表达系统,为重组牦牛Cu,Zn-SOD作为注射用药奠定基础。【方法】RT-PCR扩增牦牛Cu,Zn-SOD编码基因,构建Cu,Zn-SOD/pET22b(+)重组表达载体,并转化到E.coli BL21(DE3)宿主菌,经IPTG进行诱导表达。表达产物经SDS-PAGE、活性染色及邻苯三酚法进行检测,采用BradFord方法测定融合蛋白浓度。【结果】本试验成功获得了牦牛Cu,Zn-SOD cDNA,长456bp,编码152个氨基酸。与普通牛SOD编码基因序列cDNA一致率为99.6%,氨基酸序列一致率为98.7%,表达产物分子质量约为32kD。酶粗提取液比活约为35U·mg-1。重组蛋白浓度0.2772(mg·mL-1)。【结论】本研究成功构建了牦牛Cu,Zn-SOD/pET22b(+)原核表达载体,表达量较高、稳定性强,重组表达产物具有较高生物学活性。