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111.
In vitro viability of cryopreserved equine embryos following different freezing protocols. 下载免费PDF全文
P Poitras P Guay D Vaillancourt N Zidane M Bigras-Poulin 《Canadian journal of veterinary research》1994,58(4):235-241
The main objective of this study was to evaluate two freezing protocols and the effect of agar embedding on survival of day 6.5 equine embryos. A total of 133 embryos were used, in one group (n = 51), embryos were first embedded in agar before the freezing protocol was started. A freezing protocol to -30 degrees C or -33 degrees C was used before plunging embryos into liquid nitrogen (LN2). The embryos were thawed in water at 37 degrees C, evaluated and placed in culture. After 24 h culture, the embryos were evaluated for their morphology and development. No differences were observed between embryos plunged at -30 degrees or at -33 degrees C in LN2. The analysis of the morphology and development after thawing showed that the diameter and developmental stage at freezing correlated with embryo survival. Morula and early blastocyst stages of development were associated with better quality after freezing and thawing and had a better potential to survive after in vitro culture (p < 0.05) compared to more advanced stages. The agar failed to protect embryos from zona pellucida damage, but a tendency to prevent rupture was observed in larger embedded embryos. 相似文献
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采用正交试验法,在 pH、盐浓度,放置时间、加热条件四个因素上探讨小(?)碱的提取条件,结果用了1/2的试验时间和较少的实验用试剂,比较满意地找到了最佳提取条件。 相似文献
116.
D. M. Zhao S. Tateyama N. Miyoshi K. Uchida R. Yamaguchi T. Yamagami T. Hayashi 《Research in veterinary science》1995,59(3)
Cloning of the canine yes oncogene was attempted from a c
library derived from a healthy canine spleen using a human c-yes-1 probe. The nucleotide and amino acid sequences revealed that the canine yes gene contained an open reading frame consisting of 539 amino acids. Its product had a molecular mass of 60,368 Daltons and showed 95·9 per cent and 90·4 per cent homology with human and chick p61c-yes, respectively. Moreover, the product had a myristylation signal, src homology region (SH) 3, SH2, and tyrosine kinase domains showing 98·8 per cent and 96·0 per cent homology with those of human beings and chickens, respectively. These findings indicate that the products of the canine yes gene may have non-receptor-type tyrosine kinase activity on the cell membrane, as is the case in human and chick p61c-yes 相似文献
117.
本文根据胶合板厂实际情况,建立了杨木胶合板厂生产规划模型。该模型考虑各主要设备工段、产品组合、产品混合、产品价格、流动资金、原材料供应以及市场约束等问题。本模型能够满足市场竞争,获取最佳利润,同时还能解决胶合板厂“三板”(表、背、芯单板)不平衡问题。 相似文献
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Ihle JN 《Science (New York, N.Y.)》1987,237(4818):1060-1061
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1. Brown egg layers were fed diets differing in oil content, oil source and linoleic acid concentration and the effect on performance from 22 to 69 weeks of age was studied.
2. The birds responded with an increasing mean egg weight up to a linoleic acid intake of 2.75 g/bird d.
3. Increasing the intake of readily absorbable oil without increasing linoleic acid concentration did not increase mean egg weight. 相似文献