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981.
982.
一步法RT-PCR和两步法RT-PCR对马铃薯病毒诊断研究的比较 总被引:2,自引:0,他引:2
本研究采用试剂盒和Total试剂两种方法从马铃薯叶片和茎秆中提取总RNA,同时设计了一步法RT-PCR和两步法RT-PCR的反应体系,依据马铃薯X病毒、马铃薯Y病毒、马铃薯S病毒、马铃薯A病毒以及马铃薯卷叶病毒的基因组RNA保守序列设计特异性引物对,并运用两种RT-PCR反应体系对染病的马铃薯进行病毒检测,结果与报道的这五种马铃薯病毒核苷酸序列进行BLAST比对, 表明扩增的五种马铃薯病毒靶带的序列与靶序列完全一致。两种诊断方法对马铃薯病毒核酸进行浓度检测,结果证明,两步法RT-PCR具有较高灵敏性。但这两种方法均可快速、简便、有效地诊断马铃薯病毒。 相似文献
983.
乙醛脱氢酶基因(ALDH)为目的基因,构建了pBI-ALDH 植物表达载体,番茄品系03HN31子叶为外植体,经发根农杆菌(Agrobacterium rhizogens)介导采用共培养法转化番茄。通过分子生物学技术(PCR、Southern杂交、RT-PCR)和抗逆相关生理指标测定(相对电导率及丙二醛含量)相结合的方法对转化番茄植株进行检测,研究结果表明:乙醛脱氢酶基因( ALDH )导入并整合到番茄的基因组中,在转录水平上可以稳定表达;在干旱、高盐和低温胁迫条件下,转基因番茄植株和对照株的相对电导率和丙二醛含量有差异,证明转化番茄植株的质膜受损程度有所降低,抗氧化胁迫性能有所提高,转化率为10.78%。 相似文献
984.
A major factor affecting spring canola (Brassica napus) production in Canada is killing frosts during seedling development in the spring and seed maturation in the fall. The objective
of this study was to explore the possibility of producing spring canola lines with mutations that have altered biochemical
pathways that increase cold tolerance. The approach was to generate UV point mutations in cultured microspores followed by
chemical in vitro selection of individual mutant microspores or embryos resulting in measurable alterations to various biochemical
pathways with elevated levels of key defense signaling molecules such as, salicylic acid (SA), p-Fluoro-d,l-Phenyl Alanine (FPA), and jasmonic acid (JA). In addition, since proline (Pro) is known to protect plant tissues in the cold-induced
osmotic stress pathway, mutants that overproduce Pro were selected in vitro by using three Pro analogues: hydroxyproline (HP),
azetidine-2-carboxylate (A2C); and, 3,4-dehydro-d,l-proline (DP). Of the 329 in vitro selected mutant embryos produced, 74 were identified with significant cold tolerance compared
to their donor parents through indoor freezer tests at −6°C, and 19 had better winter field survival than winter canola checks. All chemically selected mutant doubled haploids with increased
cold tolerance compared well with parent lines for all seed quality and agronomic parameters. Development of increased frost
tolerant cultivars should allow for spring canola to be produced in western Canada without compromising seed quality. 相似文献
985.
通过调查分析,结合实地考查,对藠头产业应对日本"肯定列表制度"进行了系统的分析和总结,并提出了相关建议。 相似文献
986.
Mun Sup Yoon Jeongran Lee Chang Yung Kim Jung Hoon Kang Eun Gi Cho Hyung Jin Baek 《Euphytica》2009,165(1):69-77
Approximately 7,000 accessions of Korean soybean (Glycine max (L.) Merrill) landraces, largely composed of three collections, the Korea Atomic Energy Research Institute’s soybean (KAS),
the Korean Crop Experiment Station’s soybean (KLS) and the Korean Agricultural Development and Technology Center’s soybean
(KADTC) collections, have been conserved at the Rural Development Administration (RDA) genebank in Korea. The accessions within
collections were classified based on their traditional uses such as sauce soybean (SA), sprouted soybean (SP), soybean for
cooking with rice (SCR), and OTHERS. A total of 2,758 accessions of Korean soybean landraces were used to profile and to evaluate
genetic structure using six SSR loci. A total of 110 alleles were revealed by at the six SSR loci. The number of alleles per
SSR locus ranged from 9 to 39 in Satt187 and Satt_074, respectively. The number of alleles ranged from 87 in the KADTC collection
to 96 in the KLS collection, and from 63 in the SCR group to 95 in the SP group. Nei’s average genetic diversity ranged from
0.68 to 0.70 across three collections, and 0.64 to 0.69 across the usage groups. The average between-group differentiation
(G
st) was 0.9 among collections, and 4.1 among the usage groups. The similar average diversity among three collections implies
that the genetic background of the three collections was quite similar or that there were a large number of duplicate accessions
in three collections. The selection from the four groups classified based upon usage may be a useful way to select accessions
for developing a Korean soybean landrace core collection at the RDA genebank. DNA profile information of accessions will provide
indications of redundancies or omissions and aid in managing the soybean collection held at the RDA genebank. The information
on diversity analysis could help to enlarge the genetic diversity of materials in breeding programs and could be used to develop
a core collection. 相似文献
987.
库尔勒香梨保鲜状况及贮藏中的新问题 总被引:1,自引:0,他引:1
库尔勒香梨是新疆特色的传统优势梨,不少学者认为库尔勒香梨是瀚海梨和鸭梨的自然杂交种。近年来,它已成为中国香港、东南亚市场上具有较强竞争力的水果。但是库尔勒香梨在贮藏过程中存在很多问题,严重影响了其销售量和经济效益,大大降低了商品价值,带来了较大的经济损失。 相似文献
988.
植物冠层分析仪测量作物叶面积指数存在高估或低估现象,不同仪器的测量结果均与真实值存在差异,这种差异除了与仪器有关,还在不同的作物物候期表现出不同规律。本研究使用LAI-2000、Sunscan两种主流植物冠层分析仪测量夏玉米不同生育期LAI,并与LI-3000C叶面积仪实测结果比较。研究发现:当玉米LAI<1时,LAI-2000测量结果高于实际值,Sunscan测量结果与实际值无显著差异;12时,两种冠层分析仪测量结果均低于实际叶面积指数;不同LAI范围内,LAI-2000、Sunscan测量值与实际值的相关系数不同,最高可达0.9778和0.9637,通过建立测量值和实际值的经验关系模型,对仪器测量值进行校正可减少误差。 相似文献
989.
990.