Immunological and pathological investigations in equine experimental uveitis

Background

The pathogenic mechanism of equine recurrent uveitis (ERU) is still poorly defined and many variations between experimental animal models and spontaneous disease exist.

Objectives

The aim of our study was to investigate if Th17 cell-mediated response plays role in the pathogenesis of the used experimental model in horses and to reveal its pathological findings.

Methods

Experimental uveitis was induced in 6 healthy horses. The concentrations of retinal autoantigen CRALBP and IL-17 were measured using ELISA in aqueous humor and vitreous body of the 12 inflamed eyes as well as in 12 control non-inflamed eyes taken from 6 horses in slaughter house. After centrifugation of the two eye media, smears were prepared and cytological investigation was performed. Tissue specimens were taken from all eye globes and were submitted to histopathological investigation.

Results

CRALBP and IL-17 concentrations were significantly elevated in eye media of horses with experimental uveitis in comparison with controls. Cytological and histopathological findings corresponded to the changes characteristic of chronic immune-mediated inflammation with mononuclear cell infiltration of uvea, choroid, retina, and eye media as well as severe retinal destruction.

Conclusions

Our study demonstrated the involvement of the retinal autoantigen CRALBP as well as IL-17 in the pathogenesis of experimental uveitis in horses. These findings suggests that this experimental uveitis in horses may serve as a suitable animal model for investigation of IL-17- mediated immune response during spontaneous autoimmune uveitis in horses as well as in humans.

     

Effects of Compound Plant Nutrients on Rumen Fermentation Parameters,Rumen Microbiota and Fatty Acid Composition of Longissimus dorsi Muscle in Finishing Small Tail Han Sheep北大核心CSCD

This study was conducted to investigate the effects of compound p1ant nutrients (CPN) on rumen fermentation parameters, rumen microbiota and fatty acid composition of longissimus dorsi musc1e in finishing sma11 tai1 Han sheep. Sixteen 4-month-o1d finishing sma11 tai1 Han sheep with an initia1 body weight (BW) of (24.18±0.31) kg were random1y divided into two groups, name1y, with 8 rep1icates per group and 1 sheep per rep1icate. The sheep in the contro1 group were fed a basa1 diet, whereas the sheep of the contro1 group (CPN group) was fed the basa1 diet supp1ementation with 3‰ CPN. The experiment 1asted for 97 days after 7 days adaption. The resu1ts showed as fo11ows: compared with the contro1 group, 1) adding CPN decreased the concentration of ammonia nitrogen (NH3-N) (P<0.05); 2) CPN supp1ementation affected beta diversity of rumen microbiota; 3) the re1ative abundance of Acidobacteriota, Erysipe1otrichaceae_UCG-002, Lactobacillus, and Megasphaera were enhanced by adding CPN (P<0.05), whereas, the re1ative abundance of Bacteroidetes and Prevotella was 1ower (P<0.05); 4) the supp1ementation of CPN had no significant effect on fatty acid composition of longissimus dorsi musc1e of fattening sma11-tai1ed Han sheep (P>0.05); 5) the contents of tota1 MUFA, C18:1n9c, C14:1, C16:1, C18:2n6c, C18:3n3 and n-3PUFA in the longissimus dorsi musc1e were corre1ated with the re1ative abundance of Megasphaera, Erysipe1otrichaceae_UCG-002, Succiniclasticum and Ruminococcus (P<0.05). In conc1usion, CPN can regu1ate the rumen microbiota structure and reduce the rumen NH3-N concentration of fattening sma11-tai1ed Han sheep. In production practice, CPN can be used as a rumen eco1ogica1 regu1ator. © 2023 Authors. All rights reserved.     

A MORE GENEEAL PRODUCTIVE FORCES DISTRIBUTION MODEL WITH ENTROPY CONSTRAINT

The productive forces distribution model with entropy constraint is established. it satisfies both the demand with minimization of total cosls and the other demands with optimum distribution at a certain level. The model's regular optimal solution is a gravity molel..     

A Globally Convergent Algorithm for Nonlinear Programming

A new globally convergent algorithm was presented for a continuous differen-tiable nonlinear programming by defining a measure function deviating from the Kuhn-Tucker point. With this algorithm which can be used to get the optimum solution of the problem,the optimal lagrangian multiplier corresponding the optimal solution of the problem was also obtained. A new iterative algorithm for quadratic programming is obtained when applying the general algorithm to quadratic programming. Finally,a numerical example was given.     

RESEARCH ON A KIND OF GREY LINEAR PROGRAMMING AND THEIR DUAL THEORIES

In this paper, a kind of grey linear programming with grey interval coefficients is discussed,the definitions of the first and second whitenized linear programmlngs and the grey dual linear programmings are presented, the relationships between the solutions of the above three kinds of programming and the solution of the primal programming are explored, and some new conclusions are obtained.     

A Dynamic Model for Generation Expansion Planning and Algorithm

This paper discusses the problem of long-range generation expansion planning. A dynamic model for long-range expansion planning is set up and an algorithm-a two-step programming is presented, which is used here in a case.     

Prediction of the Economic Development for the Circumjacent Reservoir Region of the Longtan Hydropower Project Using Grey Quantitative Analog

In order to overcome some technical difficulties,we adopt ingenously the grey models combined with the quantitative analog method,and select the Dan Jiangkou Project which has accumulated practical operation data for a long time as the analog project to predict the economic influence for the circumjacent reservoir region of the Longtan hydropower project.On the basis of analog we have built various industrial economic grey models,and compare them with the corresponding predictions for the unconstructed reservoir case.The predictions of the former are greatly larger than the later ones.and hence provides a certain scientific basis for the central leaders to make a decision for constructing the huge hydropower project ultimateiy.     

The Feasible Control for the Day-operation of the Three-Gorge Cascade Hydropower Station

The feasible control problem is presented for the day-operation of the Three-Gorge Caseade Hydropower Station. The significance of the problem is explained. Basic propetties ofthe problem and the approaches to study it are discussed. Two methfods are given for solving theproblem. which are calculus of variation and linear programming. In the former. the Euler equationwith time-lag and the generalised two-point boundary conditions are obtained and the correspondingpractical implication is interpreted. In the latter. the rationality for the discrete model is exptainedand the computalional details are given for the implement software. some questions which should bepaid much attentfon and the corresponding propeels are prasented based on the numerical results andtheoretical analysis