Revelation of Rural System of Medical Services in BRICS to China

In recent years, the great achievements of basic medical and healthcare services of BRICS have gained great attention and acknowledgement from the whole world. In their medical system reform, India, Br...     

Analysis of Inter-Individual Bacterial Variation in Gut of Cicada Meimuna mongolica (Hemiptera: Cicadidae)

Intestinal bacterial community plays a crucial role in the nutrition, development, survival, and reproduction of insects. When compared with other insects with piercing-sucking mouthparts, the habitats of cicada nymphs and adults are totally different. However, little is known about the differences in the gut bacterial communities in the nymphs and adults within any cicada species. The diversity of bacteria in the gut of nymphs and adults of both genders of Meimuna mongolica (Distant) was studied using the denaturing gradient gel electrophoresis (DGGE) method. Few inter-individual variations among gut microbiota were observed, suggesting that M. mongolica typically harbors a limited and consistent suite of bacterial species. Bacteria in the genera Pseudomonas and Enterobacter were the predominant components of the gut microflora of M. mongolica at all life stages. Bacteria of Pantoea, Streptococcus, and Uruburuella were also widespread in the cicada samples but at relatively lower concentrations. The relative stability and similarity of the PCR-DGGE patterns indicate that all individuals of this cicada species harbor a characteristic bacterial community which is independent from developmental stages and genders. Related endosymbionts that could be harbored in bacteromes of cicadas were not detected in any gut samples, which could be related to the cicada species and the distribution of these endosymbionts in the cicada cavity, or due to some of the possible limitations of PCR-DGGE community profiling. It is worthwhile to further address if related cicada endosymbiont clades distribute in the alimentary canals and other internal organs through diagnostic PCR using group-specific primer sets.     

黄连及黄连须、黄连茎叶体外抗氧化活性的谱效关系研究

测定黄连与黄连须、黄连茎叶主要生物碱含量并建立高效液相(HPLC)指纹图谱,分析其抗氧化活性的谱效关系。采用HPLC分析比较黄连及黄连须、黄连茎叶中小檗碱、表小檗碱、药根碱、黄连碱、巴马汀含量差异并建立指纹图谱,通过羟自由基(·OH)、1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2-联氮基双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)自由基体外清除试验与总还原力试验评价其体外抗氧化活性差异,应用Pearson相关系数法分析HPLC指纹图谱与抗氧化活性的谱效关系。结果发现小檗碱、巴马汀含量为黄连>黄连茎叶>黄连须;药根碱、黄连碱和表小檗碱含量为黄连>黄连须>黄连茎叶;总还原力为黄连>黄连茎叶>黄连须,·OH、DPPH、ABTS·+自由基清除力为黄连茎叶>黄连>黄连须;总还原力与各峰均有较强相关性,·OH、DPPH、ABTS·+自由基清除力与4号峰相关性较强。说明黄连茎叶体外抗氧化活性较强,黄连须与黄连茎叶均有一定药用开发价值。     

立足大国农业,培养"养猪达人""猪生产技术"课程思政教学改革探索

文章以畜牧兽医类专业核心课程“猪生产技术”育人目标为出发点,结合“课程思政”的教育理念,阐述“猪生产技术”课程思政实施必要性,探索课程思政元素挖掘和教学实施,将爱国情怀、生态保护、责任担当、职业素养、团队协作、食品安全等思政元素融入课程教学过程中,旨在探索专业课程内容与思政内容融合要点,从而提高学生的专业素养、职业素养和道德水平。     

以沼气为纽带的西藏农牧业清洁生产模式研究

为了解决因长期不合理施用化肥导致的西藏土壤理化性状、营养元素、有机物质、生物学活性和重金属含量等问题,提出了以沼气为纽带的西藏农牧业清洁生产的3种模式,均以沼肥综合利用促进沼气发展为目标的,集养殖、沼气、种植为一体的循环农牧业清洁生产模式。同时,阐述了农牧业清洁生产的内涵和目标,以及西藏发展农牧业清洁生产的条件、模式、存在的问题及解决对策,为解决长期不合理施用化肥而导致的一系列问题提供思路。该研究有利于实现资源循环利用,实现生态农业与无公害农产品的生产,保护农牧区生态环境,促进西藏农牧业的可持续发展。     

Comparison of the rebound tonometer (TonoVet) with the applanation tonometer (TonoPen XL) in normal Eurasian Eagle owls (Bubo bubo)

OBJECTIVE: To examine the feasibility and accuracy of a handheld rebound tonometer, TonoVet, and to compare the intraocular pressure (IOP) readings of the TonoVet with those of an applanation tonometer, TonoPen XL, in normal Eurasian Eagle owls. ANIMALS STUDIED: Ten clinically normal Eurasian Eagle owls (20 eyes). PROCEDURES: Complete ocular examinations, using slit-lamp biomicroscopy and indirect ophthalmoscopy, were conducted on each raptor. The IOP was measured bilaterally using a rebound tonometer followed by a topical anesthetic agent after 1 min. The TonoPen XL tonometer was applied in both eyes 30 s following topical anesthesia. RESULTS: The mean +/- SD IOP obtained by rebound tonometer was 10.45 +/- 1.64 mmHg (range 7-14 mmHg), and by applanation tonometer was 9.35 +/- 1.81 mmHg (range 6-12 mmHg). There was a significant difference (P = 0.001) in the IOP obtained from both tonometers. The linear regression equation describing the relationship between both devices was y = 0.669x + 4.194 (x = TonoPen XL and y = TonoVet). The determination coefficient (r(2)) was r(2) = 0.550. CONCLUSIONS: The results suggest that readings from the rebound tonometer significantly overestimated those from the applanation tonometer and that the rebound tonometer was tolerated well because of the rapid and minimal stress-inducing method of tonometry in the Eurasian Eagle owls, even without topical anesthesia. Further studies comparing TonoVet with manometric measurements may be necessary to employ rebound tonometer for routine clinical use in Eurasian Eagle owls.     

PTGS/ RNAi的作用机制及其应用

论述了双链RNA对同源基因的表达进行特异性的封闭而引起的转录后的基因沉默（PTGS/ RNAi）现象, PTGS/ RNAi的作用机理，作用模型，参与这一过程的酶、基因、起始因子和作用因子，介绍了PTGS/ RNAi在基因组功能研究、基因的时空表达调控以及在作物改良等方面的应用。     

齿轮三维模型的参数化设计技术

齿轮是汽车、拖拉机中常用的传动零件,在三维设计中,需要对渐开线方程进行转换,由转换后的方程生成齿形,其整个设计过程非常繁琐。本文详述了方程的转换过程及齿轮的参数化设计方法,可以通过输入不同的齿轮参数,即可获得相应的齿轮,从而大大节约设计时间。该方法还可应用于所有系列化的通用件和标准件三维设计中。     

基于直剪试验的稻谷粮堆力学性质研究

为揭示粮仓建设和粮食运输途中粮堆的强度与剪胀特性,开展了在垂直压力为25~200kPa、含水率分别为8.41%、10.59%、13.88%和16.25%条件下的稻谷直剪试验。结果表明:稻谷粮堆剪切变形可分为弹性变形、塑性变形和籽粒压缩3个阶段;不同含水率下,稻谷粮堆强度特性基本符合莫尔-库伦强度准则,随着含水率增加,稻谷粮堆粘聚力逐渐减少,内摩擦角逐渐增大;含水率对稻谷粮堆的剪胀特性影响不明显,但随着垂直压力的增大,其剪缩性越明显;在垂直压力较小的条件下,含水率对稻谷粮堆的抗剪强度影响较大,随着垂直压力的增大,含水率对抗剪强度的影响逐渐减小。研究结果可为粮食仓储和运输、装载粮食机械的设计、粮堆数值仿真建模提供参考。     

变温处理对百合生长发育影响及刺足根螨防治效果

以“索邦”百合(Lilium spp.)的休眠种球为材料,经不同温度和时间的温水浸泡处理,观测百合种球的发芽率、出苗率和营养生长期、盛花期的形态指标以及鳞茎中刺足根螨(Rhizogl yphus echinopus)的发生情况,探究变温处理对百合生长发育的影响和剌足根螨的防治效果.结果 表明:百合种球以48℃温水浸泡3...