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101.
L. Gan Y.‐J. Liu L.‐X. Tian H.‐J. Yang Y.‐R. Yue Y.‐J. Chen J.‐J. Liang G.‐Y. Liang 《Aquaculture Nutrition》2012,18(6):589-598
A 63‐day growth trial was undertaken to estimate the effects of supplemented lysine and methionine with different dietary protein levels on growth performance and feed utilization in Grass Carp (Ctenopharyngodon idella). Six plant‐based practical diets were prepared, and 32CP, 30CP and 28CP diets were formulated to contain 320 g kg?1, 300 g kg?1 and 280 g kg?1 crude protein without lysine and methionine supplementation. In the supplementary group, lysine and methionine were added to formulate 32AA, 30AA and 28AA diets with 320 g kg?1, 300 g kg?1 and 280 g kg?1 dietary crude protein, respectively, according to the whole body amino acid composition of Grass Carp. In the groups without lysine and methionine supplementation, weight gain (WG, %) and specific growth rate (SGR, % day?1) of the fish fed 32CP diet were significantly higher than that of fish fed 30CP and 28CP diets, but no significant differences were found between 30CP‐ and 28CP‐diet treatments. WG and SGR of the fish fed 32AA and 30AA diets were significantly higher than that of fish fed 28AA diets, and the performance of grass carp was also significantly improved when fed diets with lysine and methionine supplementation (P < 0.05), and the interaction between dietary protein level and amino acid supplementation was noted between WG and SGR (P < 0.05). Feed intake (FI) was significantly increased with the increase in dietary protein level and the supplementation of lysine and methionine (P < 0.05), but feed conversion ratio (FCR) showed a significant decreasing trend (P < 0.05). Two days after total ammonia nitrogen (TAN) concentration test, the values of TAN discharged by the fish 8 h after feeding were 207.1, 187.5, 170.6, 157.3, 141.3 and 128.9 mg kg?1 body weight for fish fed 32CP, 32AA, 30CP, 30AA, 28CP and 28AA diets, respectively. TAN excretion by grass carp was reduced in plant‐based practical diets with the increase in dietary protein level and the supplementation of lysine and methionine (P < 0.05). The results indicated that lysine and methionine supplementation to the plant protein sources‐based practical diets can improve growth performance and feed utilization of grass carp, and the dietary crude protein can be reduced from 320 g kg?1 to 300 g kg?1 through balancing amino acids profile. The positive effect was not observed at 280 g kg?1 crude protein level. 相似文献
102.
Genomic structure and molecular characterization of growth hormone and its expression response to different feed types in golden pompano Trachinotus ovatus (Linnaeus, 1758) 下载免费PDF全文
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The objective of this study was to determine diversities of ascorbic acid (AsA) and activities of enzymes involved in AsA metabolism, and to determine their relationships to AsA content in the leaves of Chinese persimmon (Diospyros kaki) cultivars. Results showed that there were huge differences not only in the contents of AsA and glutathione (GSH) but also in the enzyme activities of l-galactono-1,4-lactone dehydrogenase (GLDH, EC 1.3.2.3), dehydroascorbate reductase (DHAR, EC 1.8.5.1), monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) in the leaves of 22 Chinese persimmon cultivars. Control of AsA levels mainly depended on the capacity for biosynthesis and GLDH activity played a main role in determining AsA levels in the leaves of persimmon. Both MDHAR and DHAR, MDHAR in particular, also played an important role in maintaining AsA redox state by reducing oxidized AsA back to AsA. But contents of GSH and hydrogen peroide (H2O2) and activity of APX were not significantly correlated with AsA content in the leaves of persimmon. 相似文献
106.
AIM: To explore the effect of rAAV2-NTF2 on the blood-retinal barrier damage in early stage diabetic retinopathy. METHODS: Retinal vasculature was observed by Evans blue. NTF2 gene was cloned into adeno-associated virus vector pSNAV. The recombinant pSNAV-NTF2 was transfected into BHK cells. After purification, high-titer rAAV2-NTF2. rAAV2-NTF2 at dose of 4 μL (titer 1.0×1012) were injected into left eyes and rAAV2-EGFP at the same does and titer were injected into the right eyes of 36 rats. After 1 month, diabetes mellitus were induced by STZ. One month after onset of diabetes, EB at a dose of 45 mg/kg was intravenously injected into the rats. Two hours later, both eyes were enucleated immediately after perfusion of 1% PFA-citric acid buffer solution. The retinas were then dissected away and placed in formamide to extract the dye. The concentration of dye was measured by spectrophotometer. RESULTS: Evans blue was contained in normal retinal vessels without leakage, with a very low level of background fluorescence. The vessels staining of diabetic rats’ retina showed increasing fluorescence, indicating the retina-blood barrier damage. Dye concentration, representing the degree of the BRB injury, was higher in retina of 1 month diabetic rats than that in normal rats (4.67 times, P<0.01), indicating retina-blood barrier break-down. Diabetic rats with rAAV2-NTF2 intravitreal injection showed decreased BRB breakdown (P<0.05). CONCLUSION: High expression of NTF-2 decreases the BRB dysfunction in DM rats. 相似文献
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在分析了农业装备物流体系特点的基础上,指出了目前存在的数学模型不能完全体现和描述农业装备物流体系的特点;建立了基于面向环境时延着色Petri网(EOCTPN)的农业装备物流体系数学模型;研究了不同类型农业装备物流体系对库存总成本和长鞭效应的影响程度;解决了目前存在的无法将正向物流和逆向物流有机结合在一起的问题;有效克服了基础Petri网描述此类问题出现的"节点爆炸"现象.在仿真实验的基础上,提出了应如何构建农业装备物流体系的方法. 相似文献
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七自由度车辆悬架系统的数学建模 总被引:1,自引:0,他引:1
悬架系统是机动车上的重要部件,其性能的好坏对车辆的平顺性、操纵稳定性和安全性等都有重要的影响。而悬架数学模型的精确性、合理性对悬架的研究起到决定作用。本文以汽车整体为研究对象,进行了力学分析,根据牛顿运动定律推导出悬架各部分的力学微分方程,从而建立了七自由度悬架系统模型,为汽车主动悬架的进一步研究提供了理论基础与依据。 相似文献
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