α-乙酰乳酸脱羧酶基因在枯草芽孢杆菌中的整合型表达

α-乙酰乳酸脱羧酶在啤酒生产中能加快啤酒成熟,有重要的应用价值。本研究将枯草芽孢杆菌启动子P43克隆到质粒pUC19-ALDC中的α-乙酰乳酸脱羧酶基因之前,得到重组质粒pUC19-P43-ALDC。重组质粒pUC19-P43-ALDC与质粒pMLK83-BN同源重组,筛选得到枯草芽孢杆菌整合质粒pMLK83-ALDC。用此整合质粒转化枯草芽孢杆菌1A751,挑选出新霉素抗性且无淀粉酶活性的重组菌株。此菌株用LB培养基在37℃、220r/min摇瓶培养过夜,测得α-乙酰乳酸脱羧酶活力为15.6U/mL,说明整合的α-乙酰乳酸脱羧酶基因能够在重组菌株中稳定传代和表达。本研究首次在枯草芽孢杆菌中用整合型的方式重组表达了α-乙酰乳酸脱羧酶,提出了一种有潜力的生产α-乙酰乳酸脱羧酶的新方法。     

元数据与图书馆数字资源组织管理

元数据是数字图书馆建设的关键技术,也是数字图书馆进行知识组织和管理的工具。从元数据的定义、格式、编码语言、应用等方面入手,探讨了元数据在数字图书馆资源组织管理方面的规范,提出了元数据在数字图书馆建设中应注意的问题。     

Relationship between Geographical Distribution of Pinus yunnanensis and Climate

A distribution map of Pinus yunnanensis was made according to the data on geographical distribution of P.yunnanensis by means of applying geographic information systems software ArcGIS, and the climatic parameters of the distribution areas for P.yunnanensis were determined by the climate forecast model Climate China.The relationship between the geographical distribution of P.yunnanensis and the climate was studied by adopting the indexes of Kira including the warmth index(WI),coldness index(CI) and aridity index (K),Xu’s humidity index(HI),Holdridge’s life temperature(BT),potential evapotranspiration (PET),potential evapotranspiration ratio(PER) and the single factors as the mean annual temperature, the mean temperature in January,the mean temperature in July,the mean annual precipitation, the>5℃accumulated temperature,the>18℃accumulated temperature,the maximum temperature, the minimum temperature,the temperature variation, the ratio of temperature and precipitation of the year, etc.And the Kira’s water-temperature indexes on distributional upper limit,low limit and north limit were discussed.The major climatic factors effecting the distribution of P.yunnanensis were screened out by statistical analysis software SPSS and the results showed the impact factors are in the order of temperature>humidity>the temperature difference between mean warmest month temperature and mean coldest month temperature.     

Oligochitosan inhibits Phytophthora capsici by penetrating the cell membrane and putative binding to intracellular targets

The mechanism of action of oligochitosan, which has shown great antifungal activity against Phytophthora capsiciin vitro, was studied using 2-aminoacridone-labeled oligochitosan (2-AMAC-oligochitosan) and a gel-retardation experiment. Internalization of 2-AMAC-oligochitosan in cysts, germtubes and sporangia of P. capsici was confirmed by confocal laser scan microscopy (CLSM), and the degree of uptake depended on the incubation concentration. 2-AMAC-oligochitosan localized mainly in the cytoplasm and showed no binding to both cell wall and cell membrane. Mannose, an inhibitor for oligochitosan uptake by macrophages, could not inhibit the internalization of oligochitosan in P. capsici. The gel-retardation experiment showed that oligochitosan bound strongly to DNA and RNA of P. capsici. These results indicate that oligochitosan exerts its antifungal activity by penetrating the cell membrane and putative binding to intracellular targets such as DNA and RNA.     

变分不等式的加性广义Schwarz算法

将加性广义Ｓｃｈｗａｒｚ算法推广到求解变不等式，并在适当条件下证明了其收敛性。     

磷脂的营养作用及生理调控功能

磷脂是动植物体内细胞膜和细胞器膜的骨架成分,其双分子层结构维持着膜内外物质和信息的传递,并对与膜相关的酶的活性进行调控;磷脂也是脂蛋白装配和分泌所必需的成分,在脂类代谢及其他生命活动中都起着重要作用。机体组织中磷脂含量和构成受日粮因素影响较大,而磷脂含量和构成     

FEEDING HABITS OF HAINAN ELDI''''S DEER

Hainan eldi's deer is a precious species of deer in the tropic zone,it is regardedas the first—class animal under protection in China.Based on all-year-round observation on thedeer's feeding habit in the reserve from 1985 to 1988,we discovered that Hainan eldi’s deer is aeuryphangous herbivore.They take various herbs as their main food and have fairly strongselectivity for woody plants,especially tending to eat the tender parts of plants and some fruits andflowers.Like other species of deer,they also like to lick the alkaline soil.This paper reports the re-search and evaluation on the feeding and choosing food characteristics of Hainan eldi’s deer,and onthe palatability of the main plants in the reserve.     

试验检测在公路工程中的作用及其运作

本文对公路工程中试验检测的依据、基本理论、部分测试操作技能进行了分析阐述，并对如何在工程实践中切实发挥质检机构的作用进行了探讨。     

Pattern of Kirtland's warbler occurrence in relation to the landscape structure of its summer habitat in northern Lower Michigan

Studies of the endangered Kirtland's warbler in relation to landscape ecosystems were conducted from 1986–1988 on a large wildfire-burn surrounding Mack Lake in southeastern Oscoda County, Michigan. A landscape ecosystem approach was used to distinguish low- and high-elevation segments of the landscape, as well as 11 local ecosystem types. The ecosystems were distinguished by physiography, microclimate, soil, and vegetation. The early occurrence of the warblers was strongly related to landscape structure, i.e., to the broad low- and high-elevation areas and the local ecosystem types within them. Territories of male warblers were observed in 5 of the 11 ecosystems. The five ecosystem types where warblers were observed were characterized by (1) a physiography of level or rolling terrain; (2) soil series of Grayling, Graycalm, Montcalm, or Rubicon; (3) uplands with relatively warm temperature during the breeding season; (4) vegetation dominated by low sweet blueberry, bearberry, wintergreen, northern pin oak, blue stem grasses, and hair cap moss; and (5) canopy of relatively tall, dense, and patchy jack pine and oak. Landscape structure appears to be an important factor affecting the occurrence of the warbler in its summer habitat in northern Lower Michigan.     

提高国家开发银行林业信贷资产质量促进经济增长方式转变的几点思考

通过对国家开发银行１９９４、１９９５两年林业信贷资产质量现状的回顾，指出了当前存在的贷款项目达标达产率降低和贷款本金回收难度加大、利息回收率逐年下降两个突出的问题；而认识上的偏差、政策上的不配套、贷款项目结构不尽合理、项目决策上的失误和项目经蓉管理的不善是问题的主要成因；按照转变经济增长方式的要求，转变观点调整贷款投向，按照现代化企业制度的要求建立项目管理体制，同时积极做好项目贷款条件的评审，加强项目的监管，并对已建成而未投产的项目进行实事求是的分析处理，是可以提高林业信贷资产的质量的。