全文获取类型
收费全文 | 50417篇 |
免费 | 2243篇 |
国内免费 | 5485篇 |
专业分类
林业 | 6036篇 |
农学 | 8236篇 |
基础科学 | 4713篇 |
8391篇 | |
综合类 | 14486篇 |
农作物 | 2607篇 |
水产渔业 | 1932篇 |
畜牧兽医 | 6706篇 |
园艺 | 1599篇 |
植物保护 | 3439篇 |
出版年
2024年 | 149篇 |
2023年 | 511篇 |
2022年 | 1402篇 |
2021年 | 2100篇 |
2020年 | 1824篇 |
2019年 | 1911篇 |
2018年 | 1318篇 |
2017年 | 1761篇 |
2016年 | 1732篇 |
2015年 | 2344篇 |
2014年 | 2143篇 |
2013年 | 2649篇 |
2012年 | 3110篇 |
2011年 | 3370篇 |
2010年 | 3176篇 |
2009年 | 2954篇 |
2008年 | 2734篇 |
2007年 | 2739篇 |
2006年 | 2661篇 |
2005年 | 2633篇 |
2004年 | 1186篇 |
2003年 | 973篇 |
2002年 | 798篇 |
2001年 | 830篇 |
2000年 | 1016篇 |
1999年 | 1355篇 |
1998年 | 1206篇 |
1997年 | 1021篇 |
1996年 | 948篇 |
1995年 | 874篇 |
1994年 | 870篇 |
1993年 | 778篇 |
1992年 | 717篇 |
1991年 | 584篇 |
1990年 | 490篇 |
1989年 | 394篇 |
1988年 | 260篇 |
1987年 | 187篇 |
1986年 | 94篇 |
1985年 | 74篇 |
1984年 | 51篇 |
1983年 | 38篇 |
1982年 | 37篇 |
1981年 | 21篇 |
1980年 | 25篇 |
1979年 | 10篇 |
1978年 | 12篇 |
1977年 | 9篇 |
1965年 | 9篇 |
1963年 | 9篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
961.
962.
963.
发展中的中国设施园艺 总被引:1,自引:1,他引:1
本文回顾了中国设施园艺发展的悠久历史。本世纪80年代末,已形成以塑料棚为主体,与风障畦、地膜覆畦和温室等相互配套的生产体系,其特点是因地制宜,就地取材,耗能少,节省投资,综合配套。与此同时设施园艺科学研究,学术活动也十分活跃,对工厂化育苗、无土栽培、地膜覆盖、温室结构、高产栽培技术和微机调控综合环境技术等方面开展了研究并取得成果,发表大量论文,中国农业工程学会设施园艺环境工程委员会多次组织学术交流、座谈、考察。此外设施园艺学的教学也有相应发展,在某些高等学校中设置了“农业建筑与环境工程专业”开设“设施园艺学”等课程,并举办各种短训班,培养大批技术人才。 相似文献
964.
965.
Inhibition of porcine circovirus type 1 and type 2 production in PK-15 cells by small interfering RNAs targeting the Rep gene 总被引:1,自引:0,他引:1
Porcine circovirus type 1 (PCV1) and type 2 (PCV2) are two genotypes of porcine circovirus. Both of them are presumed to be widespread in the swine population. Currently, there is no specific treatment for their infections. RNA interference (RNAi) is a sequence-specific RNA degradation mechanism mediated by small interfering RNA (siRNA), which represents a possible therapeutic application for the treatment of viral infections. In this study, three siRNA expression plasmids (pS-RepA, pS-RepB and pS-RepC) were generated to target three different coding regions of the Rep protein (Rep) of PCV. These siRNAs were used to inhibit PCV production in a porcine kidney cell line, PK-15 cells. Our results revealed that Rep gene expression was inhibited by pS-RepA, pS-RepB and pS-RepC to different degrees. Moreover, our study also showed that the production of PCV1 and PCV2 was reduced by these siRNAs. pS-RepC, which targets the middle region of Rep gene, proved to be the most efficient siRNA for inhibition of Rep expression and viral production. Taken together, our data suggest that RNAi could be investigated as a potential treatment for PCV infection. 相似文献
966.
为明确小麦矮腥黑粉菌Tilletia controversa g9890基因编码效应蛋白的生物学功能,根据小麦矮腥黑粉病菌转录组测序结果,筛选出效应蛋白g9890,通过PCR技术获得g9890基因cDNA的全长序列,并对其进行生物信息学以及亚细胞定位分析。多种生物信息学数据库分析表明,g9890基因全长为1 038 bp(包括终止密码子),共编码345个氨基酸,相对分子质量为37 353.32,理论等电点为5.02。g9890效应蛋白不稳定系数为15.94,疏水性指数为-0.312,是一种亲水性且稳定的蛋白。将g9890基因与pbin-GFP载体重组,利用冻融法转化至根癌农杆菌Agrobacterium tumefaciens GV3101中,将其注入烟草进行瞬时表达分析,并通过共聚焦激光显微镜观测该基因的定位状况,结果显示,g9890定位在细胞膜和细胞核上。 相似文献
967.
968.
M. Ryan Smith DVM DACVECC Virginie A. Wurlod MS Dr vet med DACVECC DECVECC Chin-Chi Liu MS MApStat PhD 《Journal of Veterinary Emergency and Critical Care》2023,33(6):648-655
Objective
To describe hemostatic derangements associated with canine anaphylaxis and to assess for association with syndrome severity.Design
Prospective observational study.Setting
University teaching hospital.Animals
Twenty-seven client-owned dogs, recruited from November 2018 to January 2022, diagnosed with anaphylaxis of varying severity were included. Study inclusion required presentation <6 hours after initiation of clinical signs, no medications or history of illness within the prior 2 weeks, lack of comorbidities expected to affect hemostasis, and lack of a disease state that could alternatively explain the clinical presentation.Interventions
Blood samples were collected within the first hour of presentation for CBC, serum biochemistry, prothrombin time (PT), activated partial thromboplastin time (aPTT), and viscoelastic coagulation testing for use with a cartridge-based point-of-care device.Measurements and main results
Clotting time and clot formation time were prolonged, alpha angle and maximum clot firmness were decreased, PT and aPTT were prolonged, and platelet counts were lower in severe cases compared to mild and moderate cases. There were no differences for any parameter between mild and moderate cases. The presence or absence of abdominal effusion was not associated with hemostatic status.Conclusions
Global hemostatic derangements consistent with hypocoagulability are a prominent feature of severe anaphylaxis in dogs and should be considered for routine evaluation. 相似文献969.
970.