Epostane对公兔血清睾酮、皮质醇的影响

将不同剂量的Ｅｐｏｓｔａｎｃ静脉注射成年公兔，测定注射后１、２、３、２４小时血清睾酮、皮质醇水平的变化。结果显示注射Ｅｐｏｓｔａｎｃ后公兔血清睾酮水平增加且与剂量相关；低剂量Ｅｐｏｓｔａｎｃ显著地降低血清皮质醇水平。     

季节对山羊反刍行为的影响

对１６只中卫山羊母羊的青草期和枯草期反刍行为进行观察表明，山羊在青草期，每天（２４ｈｒｓ）有１０个反刍周期，反刍持续总时间为３０４．２分，共反刍４２５．３次，每个食丸在口腔中咀嚼４３．０秒，共咀嚼４６．４次，在枯草期上述指标分别为１１个，４２０．０分，４７２．０次，５３．４秒和５９．６次，季节对山羊反刍行为的影响，本质上决定于羊采食牧草的品质和数量，优质牧草（青草期）能够缩短反刍持续总时间（Ｐ〉０     

参加央视《中国工作犬大集合》节目后两警犬再立新功

参加完中央电视台《中国工作犬大集合》节目后的工作犬,他们回到各自的岗位上,有什么突出表现呢?     

废渣固态发酵生产酵母蛋白质饲料及其产业化发展措施

文章综述了近几年来采用废渣固态发酵技术生产酵母蛋白质饲料的研究进展,对其应用前景和产业化发展措施进行了探讨,并提出制订固态发酵产的酵母粉的质量评估指标与质量标准,以规范该产品的生产、品管和销售市场。     

基于超平面构建的高光谱异常目标检测方法

高光谱遥感影像具有光谱分辨率高、图谱合一的特点,影像中的地物组成也多种多样,针对图像数据中目标出现概率较小、检测概率较低等问题,本文提出了基于超平面构建的高光谱数据目标异常检测方法。它通过对高光谱数据的结构分析,构建出高维空间中的背景超平面算子,以待测像元在其正交子空间的投影值来表征异常程度的大小。通过OMIS和AVIRIS实测数据验证,并与RX算法相比较,基于超平面构建的高光谱异常检测算法能够在多种背景环境下检测出纯度高、光谱差异大的异常像元,并具有较高的检测率和较低的虚警率,因此具备了有效性和较好的实用性。     

动物产品市场准入应纳入风险评估机制

目前,各地都实施了动物产品市场准入制,这为净化肉食市场,推广无公害动物产品,杜绝病害肉和注水肉,保障动物食品安全和人民健康,起到了关键性的作用。但外埠动物产品流通广泛、涉及面大、来源杂、产品多而无序,仅凭一张《出县境动物产品检疫合格证》,就能进入市场。笔者     

中华蜜蜂保种(资源)分子生物学评价方法

中华蜜蜂是我国独特的东方蜜蜂遗传资源,具有非常重要的利用与保护价值。随着技术的发展,基于分子生物学的保种手段也逐渐展开。本文详细阐述了蜜蜂保种的重要性,介绍了适用于蜜蜂资源评价的常用遗传标记以及适用于遗传资源保护的评价指标,为中蜂资源保护与利用工作提供理论与实践指导。     

Pharmacokinetics of sanguinarine,chelerythrine, and their metabolites in broiler chickens following oral and intravenous administration

Sanguinarine (SA) and chelerythrine (CHE) are the main active components of the phytogenic livestock feed additive, Sangrovit®. However, little information is available on the pharmacokinetics of Sangrovit® in poultry. The goal of this work was to study the pharmacokinetics of SA, CHE, and their metabolites, dihydrosanguinarine (DHSA) and dihydrochelerythrine (DHCHE), in 10 healthy female broiler chickens following oral (p.o.) administration of Sangrovit® and intravenous (i.v.) administration of a mixture of SA and CHE. The plasma samples were processed using two different simple protein precipitation methods because the parent drugs and metabolites are stable under different pH conditions. The absorption and metabolism of SA following p.o. administration were fast, with half‐life (t_1/2) values of 1.05 ± 0.18 hr and 0.83 ± 0.10 hr for SA and DHSA, respectively. The maximum concentration (C_max) of DHSA (2.49 ± 1.4 μg/L) was higher that of SA (1.89 ± 0.8 μg/L). The area under the concentration vs. time curve (AUC) values for SA and DHSA were 9.92 ± 5.4 and 6.08 ± 3.49 ng/ml hr, respectively. Following i.v. administration, the clearance (CL) of SA was 6.79 ± 0.63 (L·h^?1·kg^?1) with a t_1/2 of 0.34 ± 0.13 hr. The AUC values for DHSA and DHCHE were 7.48 ± 1.05 and 0.52 ± 0.09 (ng/ml hr), respectively. These data suggested that Sangrovit® had low absorption and bioavailability in broiler chickens. The work reported here provides useful information on the pharmacokinetic behavior of Sangrovit® after p.o. and i.v. administration in broiler chickens, which is important for the evaluation of its use in poultry.     

Effects of Wnt10b on dermal papilla cells via the canonical Wnt/β‐catenin signalling pathway in the Angora rabbit

Wnt10b is a member of Wnt family that plays a variety of roles in biological functions, including those in the development of hair follicles. To investigate the effect of Wnt10b on hair growth in the Angora rabbit and to determine the underlying molecular mechanism, we cultured dermal papilla (DP) cells with exogenous Wnt10b in vitro. We observed the expressions of downstream critical gene β‐catenin and lymphoid enhancer‐binding factor 1 (LEF1) in Wnt/β‐catenin pathway. The levels of β‐catenin mRNA and protein were higher in the Wnt10b group of DP cells than in the Control group, and the mRNA level of LEF1 in the Wnt10b group was higher than in the Control group. Moreover, translocation of β‐catenin from cytoplasm to nucleus was activated in the Wnt10b group. Furthermore, the mRNA levels of the hair follicle‐regulatory genes, insulin‐like growth factor‐1 (IGF‐1) and alkaline phosphatase (ALP), and the protein activity of ALP was also upregulated in the Wnt10b group compared to their corresponding levels in the Control group. These data suggest that Wnt10b could activate the canonical Wnt/β‐catenin signalling pathway to induce DP cells in the Angora rabbit. In addition, the proliferation of DP cells was significantly promoted when cultured with Wnt10b for 48 and 72 hr, suggesting that Wnt10b plays a pivotal role in the proliferation and maintenance of DP cells in vitro. In conclusion, this study demonstrates that Wnt10b may promote hair follicle growth in Angora rabbit through the canonical Wnt/β‐catenin signalling pathway that promotes the proliferation of DP cells.     

Association of polymorphisms for nuclear receptor coactivator 1 gene with egg production traits in the maternal line of Shaobo hens

1. The objectives of the study were to find polymorphic sites and elucidate the association between SNPs in the nuclear receptor coactivator 1 (NCOA1) gene and reproductive traits. 2. SNPs were detected by PCR-SSCP and DNA sequencing. Four SNPs were detected, including T10155007A, T10125838C, G10118492A and G10109315T. Three polymorphisms were associated with total egg production at the age of 300 d and the G10109315T polymorphism was associated with age at first egg. 3. In conclusion, the NCOA1 gene can be used as a molecular marker for reproductive traits in hens.