一种降低放养量提高产量的池塘养鱼方式

经二年试养结果表明,用1龄草鱼(规格为175克/尾左右)和团头鲂鱼种(规格为33克/尾左右)为混养的主体鱼,总放养密度控制在150—230克/米~2;实行轮捕轮放,保持合理的载鱼量;根据鱼类的营养需要,采用配合颗粒饲料同青饲料相结合,调节好营养平衡;合理施放磷肥、生石灰以调节水质,可以获得低放养量、高产量(1240—1540克/米~2)、高效益(1985年的净收益为2.644元/米~2,即每亩1775.8元)和缩短养殖周期一年的效果。     

Vero E6细胞酵母双杂交cDNA文库的构建及鉴定

To seek out proteins which interact with structural proteins of porcine epidemic diarrhea virus （PEDV） in Vero E6 cells and study the mechanisms of viral infection.We created a Vero E6 cDNA yeast hybrid library. The total RNA of Vero E6 was extracted using Trizol method, and double strands cDNA was synthesized by SMART technique. We created a Vero E6 cDNA library by using homologous recombination in yeast. The results showed that the titer of cDNA library was 9.7×10⁸ CFU with the average of inserted fragments about 1.6 kb, and the recombination rate was 87%. These data indicated that the yeast two-hybrid cDNA library of Vero E6 was successfully constructed and might be useful for screening the host proteins interacting with structure protein of PEDV.     

饲料中添加激素类等药剂促进僵鳗生长及复壮的研究

在工厂化、高密度、全人工控制的养鳗条件下,使用雌二醇、牛肝糜维生素、甲基睾丸素、强的松等药剂,可以显著地促进僵鳗的生长和复壮,在生产中可以取得较好的经济效益。同时,本试验对于露天止水式和水库网箱养鳗也有普遍的意义。     

海洋与水产相关学科SCI源期刊信息库

以表格的形式按期刊评价指标排序的方法，介绍了以２１３种期刊构成的《海洋与水产相关学科ＳＣＩ源期刊信息库》，该信息库的ＳＣＩ源期刊代表了海洋与水产学科最核心的科技期刊，对广大科研人员向世界宣传自己的学术成果，展示我国的科研实力，将起到重要的参考作用。     

“蕾果保”在棉花上的应用效果

在棉花蕾期和开花期喷施不同剂量的蕾果保,并以清水为对照,结果表明：在棉花初花期每公顷一次施用蕾果保3L效果最好,可使棉花成铃率提高25．5％、皮棉产量增加33．10％,烂铃率降低54．1％。     

油后棉种植方式与密度对产量效应的研究

以早熟品种JX0010为材料,在湖南益阳大通湖区研究了直播、漂浮育苗移栽、营养钵育苗移栽三种方式下的18000、36000和54000株/hm2三种密度对棉花生长发育及产量、品质的影响.结果表明:在同一种植密度下,生育期以直播棉最短,营养钵育苗最长,株高、果枝数、单株成铃数、籽棉产量以营养钵育苗移栽方式最大;在同一种植方式中,吐絮期、生育期随密度的增加而推迟,株高、果枝数、单株成铃数随密度增高而降低;在一定密度范围内,皮棉产量、籽棉产量随密度增高而提高;不同处理对棉花霜前花率、单铃重、衣分、籽指、衣指、纤维品质等影响不显著.     

四川省水稻种业知识产权保护现状及发展对策研究

以四川省2000-2012年水稻种业核心知识产权(水稻品种权和水稻发明专利)保护与管理状况为研究对象,重点对水稻核心知识产权的申请量、授权量、申请机构及技术领域分布等信息进行分析。在此基础上,探讨了四川水稻种业知识产权保护管理中存在的问题,并提出促进四川水稻种业知识产权保护的对策与建议。     

Lamellarin O,a Pyrrole Alkaloid from an Australian Marine Sponge,Ianthella sp., Reverses BCRP Mediated Drug Resistance in Cancer Cells

ATP binding cassette (ABC) transporters, such as P-gp, BCRP and MRP1, can increase efflux of clinical chemotherapeutic agents and lead to multi-drug resistance (MDR) in cancer cells. While the discovery and development of clinically useful inhibitors has proved elusive to date, this molecular target nevertheless remains a promising strategy for addressing and potentially overcoming MDR. In a search for new classes of inhibitor, we used fluorescent accumulation and efflux assays supported by cell flow cytometry and MDR reversal assays, against a panel of sensitive and MDR human cancer cell lines, to evaluate the marine sponge co-metabolites 1–12 as inhibitors of P-gp, BCRP or MRP1 initiated MDR. These studies identified and characterized lamellarin O (11) as a selective inhibitor of BCRP mediated drug efflux. A structure–activity relationship analysis inclusive of the natural products 1–12 and the synthetic analogues 13–19, supported by in silico docking studies, revealed key structural requirements for the lamellarin O (11) BCRP inhibitory pharmacophore.     

Bis(2,3-dibromo-4,5-dihydroxybenzyl) Ether,a Marine Algae Derived Bromophenol,Inhibits the Growth of Botrytis cinerea and Interacts with DNA Molecules

Bis(2,3-dibromo-4,5-dihydroxybenzyl) ether (BDDE) is a bromophenol isolated from marine algae. Previous reports have shown that BDDE possesses cytotoxic and antibacterial activity. In the present study, we demonstrate that BDDE displays broad-spectrum antifungal activities, especially on Botrytis cinerea. BDDE inhibits the growth of B. cinerea cultured on a solid medium of potato dextrose agar (PDA) as well as on the potato dextrose broth (PDB) medium. Moreover, BDDE decreases the incidence of fruit decay and severity of strawberries infected with B. cinerea. Further studies have revealed that BDDE decreases the germination rate and inhibits the mycelial growth of B. cinerea. The inhibition mechanisms are related to the disruption of the cell membrane integrity in B. cinerea spores and newly formed germ tubes. This study also suggests that BDDE possibly interacts with DNA via intercalation and minor groove binding. The studies provide evidence that BDDE has potential application in the control of gray mold after fruit harvest and the compound could serve as a candidate or lead template for rational drug design and for the development of antifungal agents.