全文获取类型
收费全文 | 28081篇 |
免费 | 1637篇 |
国内免费 | 2914篇 |
专业分类
林业 | 2812篇 |
农学 | 2983篇 |
基础科学 | 1664篇 |
3392篇 | |
综合类 | 11309篇 |
农作物 | 1982篇 |
水产渔业 | 1340篇 |
畜牧兽医 | 4145篇 |
园艺 | 1499篇 |
植物保护 | 1506篇 |
出版年
2024年 | 114篇 |
2023年 | 467篇 |
2022年 | 1078篇 |
2021年 | 1253篇 |
2020年 | 1231篇 |
2019年 | 1173篇 |
2018年 | 757篇 |
2017年 | 1185篇 |
2016年 | 966篇 |
2015年 | 1395篇 |
2014年 | 1463篇 |
2013年 | 1688篇 |
2012年 | 2124篇 |
2011年 | 2223篇 |
2010年 | 2027篇 |
2009年 | 1862篇 |
2008年 | 1677篇 |
2007年 | 1597篇 |
2006年 | 1291篇 |
2005年 | 1098篇 |
2004年 | 724篇 |
2003年 | 717篇 |
2002年 | 1025篇 |
2001年 | 875篇 |
2000年 | 639篇 |
1999年 | 391篇 |
1998年 | 261篇 |
1997年 | 211篇 |
1996年 | 181篇 |
1995年 | 159篇 |
1994年 | 118篇 |
1993年 | 126篇 |
1992年 | 133篇 |
1991年 | 99篇 |
1990年 | 88篇 |
1989年 | 73篇 |
1988年 | 44篇 |
1987年 | 31篇 |
1986年 | 11篇 |
1985年 | 7篇 |
1984年 | 10篇 |
1983年 | 4篇 |
1982年 | 7篇 |
1981年 | 7篇 |
1980年 | 3篇 |
1979年 | 3篇 |
1964年 | 1篇 |
1962年 | 3篇 |
1956年 | 6篇 |
1955年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 359 毫秒
141.
PP2C是一大类重要的蛋白磷酸酶,广泛参与不同的逆境胁迫响应。为了解PP2C基因在干旱响应中的功能,以亚洲棉石系亚1号为材料,利用RT-PCR方法从中克隆了GaPP2C24基因,并对该基因进行了生物信息学分析,荧光定量PCR研究了其在亚洲棉不同组织和干旱胁迫下的表达情况。结果表明,GaPP2C24基因CDS序列全长为1 251 bp,编码416个氨基酸。序列同源比对发现,GaPP2C24与其他植物的PP2C氨基酸序列有较高的相似性,其中与可可树(EOY33930.1)、黄麻(OMO91132.1)的相似性分别为85%,84%。进化分析表明,GaPP2C24与同属锦葵目的黄麻聚在一支,亲缘关系最近,与可可树的亲缘关系次之。实时荧光定量PCR结果表明,GaPP2C24基因在子叶、下胚轴、胚根、叶、茎和根均有表达,且在茎中的表达量最高,根中次之。GaPP2C24受干旱胁迫的诱导表达,其在根和叶片中的相对表达量在处理1 h后最高,分别达到对照的53.9,6.9倍,推测该基因在棉花干旱响应中有重要作用。 相似文献
142.
The objective of this study was to investigate whether feeding selenium (Se)-replete cows a Se-yeast supplement in late pregnancy affects nutrient metabolism and inflammatory response during the periparturient period. Twenty cows were randomly assigned to two groups with 10 cows each. Cows in one group received Se-yeast at 0.3 mg Se/kg DM during the last 4 weeks before calving in addition to fed a TMR containing supplemented sodium selenite at 0.3 mg Se/kg DM (Se-yeast), while cows in another group were only fed a TMR containing supplemented sodium selenite at 0.3 mg Se/kg DM (Control). Blood samples were collected and analyzed for nonesterified fatty acids (NEFA), β-hydroxybutyrate (BHBA), glucose, insulin, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, serum amyloid A (SAA), haptoglobin (Hp), and albumin. In control cows, plasma NEFA, IL-1β, IL-6, SAA, and Hp levels increased after calving, but glucose, insulin, and albumin levels decreased after parturition. Se-yeast supplemental cows had lower postpartum concentrations of NEFA, TNF-α, IL-1β, IL-6, SAA, and Hp, and higher postpartum levels of glucose, insulin, and albumin compared with control cows. The results indicate that feeding Se-replete cows a Se-yeast supplement in late pregnancy improves nutrient metabolism and attenuates the inflammatory response after calving. 相似文献
143.
144.
145.
146.
AIM: To study the effect of remifentanil on monophasic action potential and transmural dispersion of repolarization (TDR) in the 3-layer myocardium of isolated rabbit hearts. METHODS: Adult rabbits (n=18, 2.0 ~ 2.5 kg) were used to isolate the hearts for preparing Langendorff perfusion model. The hearts were randomly divided into 3 groups after perfusion with K-H solution for 15 min: the perfusion in control group (C group) continued for 60 min; the hearts in remifentanil group (R group) were perfused with 12 μg/L remifentanil K-H solution for 60 min; the hearts in remifentanil+aminophylline group (RA group) were given 60-min perfusion of 12 μg/L K-H remifentanil+30 mg/L aminophylline. The HR and 3 layers of myocardial monophasic action potential (MAP) in the left ventricular anterior wall were recorded at time points after balanced infusion for 15 min (T0), and continued perfusion for 15 min (T1), 30 min (T2) and 60 min (T3). The monophasic action potential duration of repolarization at 90% (MAPD90) and the transmural dispersion of repolarization (TDR) were calculated. The early afterdepolarization, delay afterdepolarization and arrhythmia were also observed. RESULTS: In R group, slower HR and prolonger MAPD90 and TDR at T1~T3 were observed as compared with those at T0 (P<0.05). R group showed slower HR and longer MAPD90 and TDR than C group and RA group (P<0.05). CONCLUSION: Remifentanil slows the HR, extends the MAPD90 and increases the TDR, thus being prone to induce reentry. Aminophylline makes HR faster and MAPD90 shorter, thereby reducing the TDR. 相似文献
147.
148.
149.
150.
在对传统RMP模式进行客观分析的基础上,将竞争与合作变量纳入到分析框架中,提出了修正(扩展)的RMP模式,即RMCP模式。同时,结合云南省玉溪市高古楼文化旅游小镇的规划案例对RMCP模式的操作思路与方法进行了具体说明。 相似文献