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11.
影响番茄离体培养再生的主要因素探讨 总被引:4,自引:2,他引:2
以5个番茄品种的子叶和下胚轴为外植体,对无菌苗的生理状态、激素组合、激素浓度和基因型等影响组培效果的主要因素进行探讨,建立了番茄的高效离体培养再生体系。结果表明,下胚轴和子叶均得到了高达100%的出愈率和95%以上的分化率;最佳的品种是T431;最佳激素组合为ZT1.0mg·L-1和IAA1.0mg·L-1。 相似文献
12.
红鳞蒲桃开花物候特性研究 总被引:3,自引:0,他引:3
对红鳞蒲桃种群的花期物候,单株、单个花序、单花的开花动态进行系统的观测研究,详细测量并记录花部构件,同时调查红鳞蒲桃种群的同花期植物。结果表明:红鳞蒲桃种群的花期物候为8月1日~9月15日(2008年),持续时间46d;单株花期从23d到33d不等;单花序的花期情况较为复杂,总体表现为花朵数越多,该花序持续时间越长,但也有个别情况出现,在开花中期花序持续时间长,开花后期花序持续时间最短,开花前期花序持续时间介于两者之间;在整个种群花期内单花平均开花时间约5.2d,种群开花前期单花的持续时间约为6.7d,开花中期约为4.7d,末花期约为4.3d,种群开花前期单花的持续时间显著大于种群中后期的持续时间。红鳞蒲桃种群的同花期植物隶属于16科21属22种。 相似文献
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14.
M P Yang R Goitsuka H Tsujimoto M Onuma A Hasegawa 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(4):757-761
The association of tumor-associated antigen (TAA) on the proliferation of BLV-infected lymphoblastoid B-cell lines (BL2M3 and BL312) was investigated. Flow cytometric analysis of the expression of TAA with monoclonal antibody (mAb) c143 showed high expression of TAA on the surfaces of BL2M3 and BL312 cells. A large amount of TAA was found in the culture supernatant of BL2M3 and BL312 cells as well as in the lysates of BL2M3 and BL312 cells. Culture supernatant but not lysates of BL2M3 and BL312 cells promoted the growth of either BL2M3 cells or BL312 cells. Furthermore, this growth promoting activity in culture supernatants of BL2M3 and BL312 cells was inhibited in a dose-dependent manner when cultured with mAb c143. These results suggested that TAA may be involved in the growth factor-mediated cell growth of bovine B-lymphoblastoid cell lines expressing TAA on their cell surface. 相似文献
15.
Soil pH declined from 5.9 to 5.0 in 8 years beneath plantations of Eucalyptus saligna (Sm.) in Hawaii. In stands of Albizia falcataria, (L.) Fosberg, the soil pH change was more dramatic, declining from 5.9 to 4.6. We measured several components of soil acidity beneath four mixtures of the two tree species to gain insight on the processes responsible for the decline in soil pH. These components were studied using an empirical method of comparing acid quantity, degree of neutralization (depletion of base cations), and acid strength. The decline in soil pH differed between species as a result of differences in the degree of neutralization of the soil exchange complex; the larger decrease in soil pH under Albizia was produced by greater acidification of the exchange complex. Empirical titration curves suggested that differences in acid strength moderated the divergence in soil pH beneath the species. Had the acids accumulating in the soil under Albizia been as strong as those in the Eucalyptus soil, the difference in soil pH would have been greater. Though the two species had contrasting effects on soil pH, the differences in degree of neutralization, responsible for the pH decline, were small compared with differences in the amount of cations stored in tree biomass. Continued supply of nutrient cations (from weathering or fertilization) will ultimately control both the extent of soil pH decline and the level of productivity sustained by the forest. 相似文献
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17.
J. Cabaret H. M. Baudet J. Devos J. Hubert J. Cortet C. Sauv 《Veterinary parasitology》1995,60(3-4):331-337
Multispecific resistance to benzimidazoles was studied in three selected farms. These farms had bred dairy goats for more than 15 years. The helminths were introduced with the goats at the establishment of the farms which afterwards remained isolated. Nematode resistance could then be related to their own management practices. Faecal egg count tests and egg hatch assays were performed to assess intensity of resistance. The generic (infective larvae in faecal cultures) and specific richness (adult worms) were assessed. The resistant species were Trichostrongylus colubriformis, Teladorsagia circumcincta, Haemonchus contortus and Oesophagostomum venulosum. Faecal egg count reduction tests and egg-hatch assays did not match exactly. Faecal larval counts after treatments gave a distorted picture of multispecific resistance: Haemonchus and Oesophagostomum were very largely over represented. The number of species found in the three farms was relatively low compared with other reports in goat farms of the area. This reduction of diversity might also be due in part to characteristics of breeding management and history (use of permanent pasture and introduction of goats at the establishment of farm). 相似文献
18.
江苏省盐城市所辖的射阳县洋马乡和东台市三仓镇,近几年来,在农业结构调整中,因地制宜确立主导产业,千方百计培育主导产业,分别成为远近闻名的中药材之乡和西瓜之乡,1999年洋马乡中药材种植面积达3333.3公顷,实现产值1.6亿元,分别占全乡耕地面积和种植业产值的70%和75%;三仓镇种植大棚西瓜2020公顷,实现产值1.2亿元,分别占全镇耕地面积和种植业产值的40%和64.6%. 相似文献
19.
J Tetens S A Barker M Waguespack G Hosgood 《Canadian journal of veterinary research》2001,65(2):133-135
The objectives were to use high performance liquid chromatography (HPLC) to validate an established method for adenine nucleotide separation in equine colonic mucosal tissue, to determine the inherent variability in the tissue and extraction method, and to determine the stability of ATP, ADP, and AMP in the tissue with time. Equine colonic mucosal tissue obtained from a single horse was immediately submersed in liquid nitrogen, and stored at -70 degrees C. Samples were lyophilized, extracted, and separated by HPLC. The limit of quantitation was 0.05 microg/mL. The coefficient of variation for the instrument was less than 10% for all nucleotides measured. When the tissue was not homogenized prior to sampling, there were significant differences in adenine nucleotide content between samples. However, when the tissue was homogenized prior to analysis, these differences were no longer significant. There was no significant decrease in ATP, ADP, or AMP content over a 54-day analysis period. 相似文献
20.
西南交通大学郫县新校区绿化环境设计中交流氛围的营造 总被引:3,自引:1,他引:2
现代化教育的发展使得高等学校的功能不断地扩展,对内对外交流与合作的功能正在日益显现。这要求在高校校园绿化环境设计中体现和满足这一功能。提出了在绿化环境设计中营造交流氛围的原则,并在西南交通大学郫县新校区绿化环境设计中对交流氛围的营造作了初步探索和实践。 相似文献