Involvement of Sirt1 in mouse myocardial hypertrophy induced by isorenin

AIM: To investigate the expression and function of sirtuin 1 (Sirt1), one of the class III histone deacetylases, in hypertrophied mouse myocardium induced by isorenin (ISO). METHODS: The Kunming mice were randomly divided into 3 groups: control group, ISO group and ISO+nicotinamide (NAM) group. The myocardial hypertrophy was induced by dorsal subcutaneous injection of isorenin. Nicotinamide, an inhibitor against Sirt1, was given by peritoneal injection. Heart weight index (heart weight/body weight), hematoxylin and eosin staining, transmission electron microscope and mRNA expression of brain natriuretic peptide (BNP) were observed to identify the myocardial hypertrophy. The expression of Sirt1 in mRNA and protein levels was detected by RT-PCR and Western blotting, respectively. RESULTS: Compared to control group, the results of heart weight index, hematoxylin and eosin staining, the observation of transmission electron microscopy and mRNA expression of BNP showed that the mouse myocardial hypertrophy was induced by isorenin successfully. The Sirt1 expression was increased in hypertrophy model group (P<0.01 vs control group). Treatment with nicotinamide inhibited the cardiac hypertrophy induced by ISO (P<0.05 vs ISO group) and decreased the expression of Sirt1 (P<0.01 vs ISO group). CONCLUSION: Activation of Sirt1 might be involved in the process of myocardial hypertrophy stimulated by isorenin in mice.     

直立与弯曲穗型水稻穗上不同部位籽粒淀粉RVA谱特征的比较

利用3个直立穗型和3个弯曲穗型粳稻品种研究了两种不同穗型水稻穗上不同部位籽粒淀粉RVA谱特征的差异.研究结果表明,梗稻的穗型特征与品种间的淀粉RVA谱特征值高低、穗内不同部位间的变异及粒位顺序并无直接必然的联系.同一稻穗内不同粒位间的淀粉RVA谱特征值高低与其颖花在穗上的开花顺序有一定联系,两种穗型(直立和弯曲)品种都...     

棘头梅童鱼形态特征及其相关生物学参数分析

对捕捞自闽东海域的209尾体质量7.70～66.09 g、体长79.88～150.70 mm的棘头梅童鱼的外部形态、可数性状和可量性状进行了研究,分析了形态特征参数间的相关关系。结果表明：(1)棘头梅童鱼鱼体呈长椭圆形,侧扁,背部呈浅弧形;头大而圆钝,额部隆起;口大,口裂倾斜度大;吻短钝,眼小;头部及体部被薄圆鳞,鳞小;侧线发达,略呈弧形;背侧面灰黄色,腹侧面金黄色,鳃腔上部深黑色;唇橙红色,口腔浅色。背鳍鳍棘部边缘及尾鳍末端为灰黑色,各鳍为淡黄色。(2)全长、肛前长、尾柄长、头长、眼径、头高、体高、尾柄高、眼后头长和体质量与体长存在极显著相关关系(P<0.01),体宽与体长存在显著相关关系(P<0.05)。(3)以体长为自变量、各参数为因变量进行拟合处理,结果显示,全长、肛前长、尾柄长、头长、眼径、体高、体宽和眼后头长与体长呈指数相关,而尾柄高、头高与体长呈线性相关,其中体质量(W)与体长(S_l)的拟合方程为：W=4.506 5×10^-6S_l^{3.290 7}(R²=...     

水稻籽粒镉积累KASP分子标记LCd-38的开发与利用

水稻(Oryza sativaL.)是我国主粮作物之一,稻米镉污染对我国粮食安全造成一定威胁.镉低积累水稻品种的选育可有效降低水稻籽粒镉污染风险,高效准确的镉积累相关分子标记在镉低积累水稻品种选育中具有至关重要的作用.以水稻微核心种质为材料,基于籽粒镉积累关联基因的功能性SNP位点开发了KASP分子标记LCd-38.选...     

大黄鱼冰鲜杂鱼饲料中细菌多样性研究

为研究大黄鱼（Larimichthys crocea）冰鲜杂鱼饲料中细菌的多样性,用细菌通用引物构建了细菌16S rRNA基因克隆文库。从文库中随机挑选125个克隆子进行限制性片段长度多态性（ RFLP）分析,得到82个不同的RFLP 带型。对部分代表性克隆子进行测序,得到23条有效序列。序列同源性分析和系统进化分析结果表明,大黄鱼冰鲜杂鱼饲料中细菌主要分为3大类群,其中γ-变形菌纲（γ-Proteobacteria）细菌占据明显地优势地位,约占所分析克隆子数的84.4％;其次是黄杆菌纲（Flavobacteria）细菌,约占10.9％;还有少量的梭杆菌纲（Fusobac-teria）细菌,约占4.7％。γ-变形菌纲细菌中以嗜冷杆菌（Psychrobacter sp.）最多,其次是发光杆菌（Photobacteri-um）。     

Research on a dynamic tracking model for reservoir water flooding of separated zone water injection

Waterflood is one of the main oil reservoir development ways. Premature flooding of production wells during water flooding process will reduce oil recovery. Therefore,it is particularly important to establish a dynamic tracking model for reservoir water flooding of separated zone water injection. Based on the basic principles of heat and mass transfer,according to well temperature curve to determine the water profile,Poisson process analysis and stochastic process methods are adopted to calculate water saturation,water breakthrough time and water cut of each layer in water flooded reservoir at any one time. When oil reservoir gradually become water flooded,the calculated water cut of model considering micro-pore distribution of the reservoir tends to the measured water cut,and the calculation error is less than 5%. Case study shows that the layer of the higher injection rate,with high flow channel such as micro fracture,where there is the faster increase in water saturation,the shorter water breakthrough time and faster increase in water cut,and vice versa. Research result provides time guarantee to take measures for inhibiting or delaying water breakthrough of production wells.     

IFN-γR和ER在大鼠下丘脑中的共存

为了探讨神经-免疫-内分泌网络中生物活性物质间相互调节和平衡的关系,本试验应用免疫组化双标记法对成年SD雌性大鼠下丘脑中IFN-γ受体（IFN-γR）和雌激素受体（ER）的共表达进行了研究。结果发现,IFN-γR和ER广泛存在于大鼠下丘脑中,其中在视前交叉上核、视前内侧核、室周核、交叉上核、下丘脑前核、下丘脑内侧核、下丘脑外侧核、乳头体前背侧核、乳头体前腹侧核等13个核团中存在大量的IFN-γR和ER双标记细胞,双标记细胞约占全部阳性标记细胞的60．9％;双标记细胞胞质呈黑褐色、胞核呈棕黄色;ER单标着色细胞以神经胶质细胞居多。研究结果表明,IFN-γ和雌激素可以分别以其各自受体为介质进行信号传递和信息整合,同时也通过在同一细胞中的相互作用而参与机体的神经-免疫-内分泌调节。     

宁夏固原市草畜产业发展运行机制探讨

草畜业生产方式的转变,是一项系统工程,具有复杂性和前瞻性,涉及到农、林等相关行业,与环境保护、城镇建设密切相关。本文根据当地草畜业生产实际,提出了固原市草畜业生产发展机制方面的一些创新思路。     

甘肃不同地区油松针叶氨基酸成分分析研究

油松（Pinus tabulaeformis Carr．）是近年来引入干旱地区的主要造林树种,目前甘肃省已建立万亩油松苗育基地,进行大面积示范推广,主要分布于陇东、陇南、天水等地区。油松的饲用价值和药用价值是针叶,针叶富含多种营养成分,可制成松针精油、松针软膏、松针粉、抗癌扶正冲剂、松针饮料等产品,特别是以石油醚脱脂后的松针用乙醇提取混合氨基酸,作为饲料添加剂,可以促进动物生长,增强抗病能力。但其地域不同,营养成分含量也不尽相同。     

植物衰老的分子机制及其调控

综述了植物衰老的意义和影响衰老的内部、外部因素 ,并从分子水平上探讨了植物衰老的机制及其遗传学调控