Mechanism of RhoA on vascular reactivity following hemorrhagic shock in rats

AIM: To observe the mechanisms of RhoA on vascular reactivity following hemorrhagic shock (HS) in rats. METHODS: The superior mesenteric artery (SMA) in rats subjected to hemorrhagic shock was adopted to assay the vascular reactivity via observing the contraction initiated by norepinephrine (NE) with isolated organ perfusion system. Meanwhile, the effects of Rho kinase, myosin light chain phosphatase (MLCP), myosin light chain kinase (MLCK) on RhoA regulating vascular reactivity were observed. The effects of RhoA agonist U-46619 and inhibitor C3 enzyme on the activities of Rho kianse, MLCP, MLCK and phosphorylation of MLC20 in the vascular smooth muscle cells (VSMC) with hypoxia were also measured. RESULTS: As compared to control group, the cumulative dose-response curves of SMA to NE at 2 h after shock shifted to the right, the maximal contractions (Emax) of NE was significantly decreased. RhoA agonist U-46619 increased the vascular reactivity in the late period of shock. C3 enzyme abolished U-46619 induced the increase in the contractile response of SMA to NE. Rho kinase inhibitor Y-27632 decreased U-46619-induced the increase in the vascular reactivity, MLCP inhibitor calyculin further promoted the increase in the vascular reactivity. However, MLCK inhibitor had no effect on the U-46619-induced change of vascular reactivity. After hypoxia, the activities of Rho kinase and MLCK, and the level of MLC20 phosphorylation were decreased, MLCP activity was increased. RhoA agonist U-46619 increased the activity of Rho kinase and phosphorylation of MLC20, decreased the activity of MLCP, but had no effects on MLCK activity. CONCLUSION: RhoA plays an important role in the regulation of vascular reactivity following shock. The mechanism is closely related to regulating the activities of Rho kinase and MLCP, and increasing the phosphorylation of MLC20 in VSMC.     

Adrenomedullin gene transfection enhances the therapeutic effects of transplantation of bone marrow mesenchymal stem cells on cardiac function and ventricle remodeling in acute myocardial infarction rats

AIM: To investigate adrenomedullin gene transfection enhances the therapeutic effects of homogeneous transplantation of bone marrow mesenchymal stem cells (MSCs) on cardiac function and ventricle remodeling in acute myocardial infarction rats. METHODS: MSCs were isolated and expanded using the preplating method. The infection efficiency of adenovirus vector to MSCs was tested by X-gal staining. Ad-ADM expression in MSCs and its secretion in culture medium were measured by ELISA. The left anterior descending branch of rats was ligated to establish a myocardial infarction model. The MSCs were labeled by DAPI, and were directly implanted into the acute infarct site via focal injection. Four weeks later, cardiac function was evaluated using physiological recorder. Hearts were harvested and sliced to be analyzed by immunohistochemistry (factor Ⅷ and ADM) and the DAPI-labeled cells were identified. Sirius red staining was used to identify interstitial collagen on slides. Analysis of collagen type I and III was performed using a polarized filter on sections stained for collagen with Sirius red, and the ratio of collagen type I and III were detected. RESULTS: With X-gal staining, MSCs were effectively transfected by adenovirus in vitro. The transfection efficiency showed the dose-effect relationship with multiplicities of infection (MOI). When MOI was 150, the infection efficiency was 95.4%. The expression of ADM was traced in culture medium and expressed in the time-dependent manner. A maximum production of ADM was observed at 7 d after infection ［(26.53±1.42) ng/L vs (1.34±0.08) ng/L, P<0.05］, and ADM secretion reduced to normal level at 15 d ［(2.20±1.44) ng/L vs (1.52±0.33) ng/L, P>0.05］. DAPI-labeled MSCs transplantation was found in the hearts of the recipients. Immunohistochemical studies demonstrated that intense immunostaining for ADM was higher in Ad-ADM plus MSCs group, compared to other groups. Compared with control, MSCs transplantation significantly increased capillary density in infarct area (P<0.01). A combination of Ad-ADM trensfection and MSCs transplantation demonstrated a further increase in capillary density compared with Ad-ADM or MSCs alone. MSCs transplantation decreased the ratio of collagen type I and III, obviously improved the left ventricular functions. Furthermore the combination treatment resulted in further decrease in the ratio of collagen type I and III, and significantly improved the left ventricular functions. CONCLUSION: Ad-ADM transfection enhances the angiogenic potency of MSCs transplantation and decreases the ratio of collagen type I and III through increasing ADM expression in infarct area, thus contributes to reverse the ventricular remodeling and improves the cardiac function.     

中早熟甘蓝新品种东农611的选育

东农611是以3-1028为母本,以3-1030为父本配制而成的中早熟甘蓝一代杂种。叶球近圆形,外叶灰绿色,表面平滑,中肋细,叶球紧实度高,中心柱小于球高的1/2,叶质脆嫩,VC含量452.3 mg·kg-1,抗TuMV和黑腐病,单球质量2 kg左右,每667 m²产量5 000 kg左右,适于黑龙江、吉林、内蒙古东北部等地春、秋早熟栽培。     

西瓜主要农艺性状与裂果性状的相关及通径分析

对西瓜的14个农艺性状进行相关与通径分析。相关分析结果表明,裂果率与果皮厚度、外果皮木质化程度呈显著或极显著相关。经过逐步回归,10个性状与裂果有直接和间接效应,决定系数为1.000,剩余通径系数为0.001 8。外果皮木质化程度对裂果的直接作用最大,直接通径系数达到0.796,可将其作为筛选抗裂品种的重要参考性状。     

光环境调控及LED在蔬菜设施栽培中的应用和前景

综述了近年来光环境调控及发光二极管（LED）在蔬菜设施栽培中的研究与应用,主要内容包括：我国蔬菜设施栽培中光环境现状及问题,光环境调控在蔬菜设施栽培中的应用研究进展,LED在蔬菜设施栽培中的应用前景分析。     

植物源杀菌剂大黄素甲醚防治黄瓜白粉病研究

采用温室盆栽的方法,从生产厂家提供的7种不同浓度与配比的大黄素甲醚制剂中筛选出防治黄瓜白粉病效果最佳的配比组合剂型0.5 %大黄素甲醚水剂,其稀释倍数小于等于1 000倍时,防治效果在90 %左右,显著高于其他配比组合及对照药剂三唑酮与大黄素AM。对该药剂的作用方式、保护作用持效期、稳定性、防治技术及田间效果进行了研究与验证。结果表明：0.5 %大黄素甲醚水剂兼具保护与治疗作用,以保护作用更为突出,接种后14 d保护效果仍高达94 %;其保护作用的持效期为3~4 d,在黄瓜生长期间的温度与光照条件下能保持很强的稳定性与防治活性,田间验证也取得了稳定且显著的防治效果。     

长日洋葱新品种金球的选育

金球是以雄性不育系B2354A为母本,自交系3104为父本育成的洋葱一代杂种。该品种属长日照、中晚熟 类型,生育期120 d（天）左右;平均单球质量203 g,每667 m²产量4 500 kg左右,比对照卡木依增产12.9 %, 鳞茎黄色、圆球形,肉质鳞片白色,质脆细嫩;抗病性强,较耐贮藏,适合黑龙江、吉林、内蒙古、新疆及河北 等地区春播栽培,累计推广面积1 100 hm²。     

秋播小型大白菜新品种京秋娃娃菜的选育

京秋娃娃菜是由两个自交不亲和系配制而成的秋播小型大白菜一代杂种。成熟期55 d（天）,株型半直立,株高32 cm,开展度36 cm,叶色绿,外叶数8片,球叶数38片;叶球合抱,筒形,球色绿,内叶浅黄色,叶球高24 cm,直径10 cm,球形指数2.4,叶球质量0.6 kg左右,净菜率62.4 %。每667 m2净菜产量约5 000 kg。高抗病毒病,抗霜霉病和黑腐病。适合北京、河北、山东、辽宁、黑龙江、吉林、内蒙古等地秋季栽培,目前累计推广面积400 hm²。     

茄子的组织培养和植株再生体系研究

以2个茄子品种：红茄和紫长茄的子叶和下胚轴为外植体,探讨了不同生长调节物质对外植体分化的影响和不同品种、不同外植体的分化能力差异,并建立了茄子的高频率离体再生体系。结果表明：培养基MS＋2,4-D 1.0 mg/L＋6-BA 0.5 mg/L诱导愈伤组织效果最好;诱导不定芽方面,培养基MS＋4-PU 0.1 mg/L诱导效果最好;1/2MS培养基较MS培养基更适合植株生根。     

不同贮前热处理对青种枇杷果实贮藏效应的研究

通过采用不同的贮前热处理温度和时间组合,研究白沙枇杷青种在低温条件下的贮藏的内外品质的变化.结果表明:3种贮前热处理中,B处理(45℃热水处理10 min+低温(6±1℃))的效果最好,不仅青种枇杷果实的失重率和腐烂率低;而且贮藏到30 d时,枇杷果实的固酸比最高,较好保持了果实的风味.