如东县鸡腿病病因的调查研究

本文对如东县４０个养鸡户的鸡腿病进行了门诊与跟踪调查。从传染病、营养代谢病及饲养管理三个方面对发病原因进行了初步的探讨。结果表明：营养缺乏和代谢紊乱（特别是钙、磷）是该县鸡腿病的主要原因，传染性腿病如疑似病毒性关节炎、禽脑脊髓炎、葡萄球菌性关节炎等也不容忽视。     

锗─132对鸡马立克氏病肿瘤病变的抑制效果及其作用机理的研究Ⅰ．锗─132对健康及人工感染MDV雏鸡抗氧化功能的影响

选用１日龄肉用雏鸡３００只，随机分为４组：Ｉ组为健康对照；Ⅱ、Ⅲ组喂１００ｐｐｍ锗─１３２（Ｇｅ－１３２）；Ⅲ、Ⅳ组人工感染马立克氏病病毒（ＭＤＶ）。经６０ｄ临床观察并每隔１０ｄ用电子自旋共振波谱（ＥＳＲ）法测定胸腺、法氏囊及脾组织中自由基含量，同时检测全血ＧＳＨ－Ｐｘ、血清ＳＯＤ活性及血清丙二醛含量。结果，健康雏鸡全血ＧＳＨ－ｐｘ活性均值为７．９３８７±１．２３７８Ｕ／ｍＬ血清ＳＯＤ活性为８０．４６４３±１．０９８７ｎＵ／ｍＬ血清丙二醛含量为２．９９７８±０１７６０ｎｍｏｌ／ｍＬ；健康雏鸡胸腺、法氏囊及脾组织中自由基平均含量分别为：０．２７±０．０４、０．３４±０．０８、０．２８±０．０６相对浓度（×１０１０ｓｐｉｎｓ／ｇ湿重）。通过与健康对照组比较，人工感染ＭＤＶ雏鸡全血ＧＳＨ－ｐｘ和血清ＳＯＤ活性明显降低，而上述各组织中自由基和血清丙二醛含量则显著增多。Ⅲ、Ⅳ两组对比结果表明：Ⅲ组的全血ＧＳＨ－ｐｘ和血清ＳＯＤ活性高于Ⅳ组，组织中自由基和血清丙二醛含量却低于Ⅳ组，从而证实Ｇｅ－１３２能增强人工感染ＭＤＶ雏鸡的抗氧化功能。     

两种一年生植物在替代试验中的相互竞争关系

（1）用替代试验研究了燕麦（Aneva sativa）和箭舌豌豆（Vicia sativa）在混播中的竞争相互作用。为了攻混播比例对两种植物在混播条件下的行为影响，田间试验设为5个混播总密度和5种混播比例，共了5次。（2）方差分析表明，相对总生物量（RYT）和竞争平衡指数（CBI）对总密度，混播比例和生长时间的反应敏感。总密度对RYT和CBI的影响高度显著，在大多数情况下，混播比例对RYT和CBI的影响不显著。（3）本文研究的结果表明，持续时间对RYT和CBI很强的影响，在第1次测定时，RYT值接近1而BI值接近0，这一事实表明，种间无竞争或互惠作用。而在生长旺盛期，种间竞争较为剧烈。（4）本研究结果表明，箭舌豌豆的生长严惩受到燕麦的抑制作用，这说明箭舌豌豆在与燕麦混播时表现为竞争上的弱者。     

The effect of exploratory laparotomy on the serum and peritoneal haptoglobin concentrations of the pony.

Serum haptoglobin concentration was used as an indicator of the acute phase response in ponies undergoing exploratory laparotomy. Preoperative, 1 h intraoperative, 3 h, 6 h, 12 h and 24 h postoperative blood samples and 48 h postoperative peritoneal fluid samples were obtained for haptoglobin analysis. A spectrophotometric assay based on cyanmethemoglobin binding capacity (CyanBC) was used to determine haptoglobin concentrations. The preoperative reference range for serum haptoglobin concentrations in these ponies was 25-60 mg CyanBC/dL. Intraoperative and 3 h postoperative blood samples had decreased haptoglobin concentrations when compared to preoperative values. Serum haptoglobin concentrations began to rise by the 6 h postoperative sample and were generally elevated above preoperative values by the 24 h postoperative sample. Two of the ten ponies had mild signs of postoperative colic which were associated with twofold elevations in serum haptoglobin concentrations and fivefold elevations in peritoneal fluid haptoglobin concentrations.     

Feline leukemia virus detection by immunohistochemistry and polymerase chain reaction in formalin-fixed, paraffin-embedded tumor tissue from cats with lymphosarcoma.

The prevalence of feline leukemia virus (FeLV) antigen and DNA was assessed in formalin-fixed, paraffin-embedded tumor tissues from 70 cats with lymphosarcoma (LSA). Tissue sections were tested for FeLV gp70 antigen using avidinbiotin complex (ABC) immunohistochemistry (IHC); DNA was extracted and purified from the same tissue blocks for polymerase chain reaction (PCR) amplification of a 166 base pair region of the FeLV long terminal repeat (LTR). Results were related to antemortem FeLV enzyme-linked immunosorbent assay (ELISA) for serum p27 antigen, anatomic site of LSA, and patient age. Viral DNA was detected by PCR in 80% of cases and viral antigen by IHC in 57% of cases. Seventeen cases were PCR-positive and IHC-negative; one case was PCR-negative and IHC-positive. Clinical records included FeLV ELISA results for 30 of 70 cats. All 19 ELISA-positive cats were positive by PCR and IHC; of the 11 ELISA-negative cats that were negative by IHC, seven were positive by PCR. When evaluated according to anatomic site, FeLV DNA and antigen were detected less frequently in intestinal LSAs than in multicentric and mediastinal tumors. Lymphosarcoma tissues from cats < 7 yr were several fold more likely to be positive for FeLV antigen by IHC than were tumors from cats > or = 7 yr. However, there was no significant difference in PCR detection of FeLV provirus between LSAs from cats < 7 yr and those > or = 7 yr.(ABSTRACT TRUNCATED AT 250 WORDS)     

饲粮不同蛋白质、能量水平对大白鼠生长发育及体成分的影响

本试验所用动物为53—57日龄雄性大白鼠(sprague——Dawley品系),初始体重为121.73±3.95g,试验期为大白鼠生长发育旺期的21天。试验拟研究不同粗蛋白水平(10％、15％、20％、25％和30％)及两个能量水平(消化能:3.6和3.9kcal/g)的饲粮对大白鼠生长发育及各种体成分的影响。结果表明,当饲粮蛋白质供应充分时,大白鼠生长随饲粮能量浓度的提高而改善;而当饲粮蛋白质供应感觉不足时,提高能量浓度对生长无益。机体各项成分指杯中,以蛋白质、脂肪、水分沉积受饲粮蛋白质,能量浓度影响显著;而蛋白质、脂肪沉积在很大程度上受饲粮能朊比的影响,饲粮中添加脂肪可使大白鼠机体肥胖。     

Quantitation of adenine nucleotides in equine colonic mucosal tissue using high performance liquid chromatography.

The objectives were to use high performance liquid chromatography (HPLC) to validate an established method for adenine nucleotide separation in equine colonic mucosal tissue, to determine the inherent variability in the tissue and extraction method, and to determine the stability of ATP, ADP, and AMP in the tissue with time. Equine colonic mucosal tissue obtained from a single horse was immediately submersed in liquid nitrogen, and stored at -70 degrees C. Samples were lyophilized, extracted, and separated by HPLC. The limit of quantitation was 0.05 microg/mL. The coefficient of variation for the instrument was less than 10% for all nucleotides measured. When the tissue was not homogenized prior to sampling, there were significant differences in adenine nucleotide content between samples. However, when the tissue was homogenized prior to analysis, these differences were no longer significant. There was no significant decrease in ATP, ADP, or AMP content over a 54-day analysis period.