Validation of the otoliths for age determination in Barbus sclateri (Günth.)

Abstract – Otoliths were used to explore the timing of annulus formation in Barbus sclateri. Lapillus exhibited opaque and translucent zones whose combination was formed once a year. Opaque zones appeared formed from July and January, and the translucent ones from February to June. We describe a procedure to assess age by using the number of annulii, the condition of the otolith's edge and the capture date. The use of otoliths for age determination in this cyprinid endemic to the Iberian Peninsula provides a useful alternative to the traditional methods of scale reading. ^Note     

Ionic Ratios and Crop Performance: II. Effects of Interactions amongst Vanadium, Phosphorus, Magnesium and Calcium on Soybean Yield

Because vanadium (V) is easily reduced to a cationic form within plant cells, data from resin-extraction of soil were analysed for evidence of interactions between V and the resin-extractable concentrations of magnesium (Mg) and calcium (Ca) on soybean seed yield. Three varieties, 9091, 9061 and 704, were grown over a 3-year period in a corn–soybean–wheat rotation. Surface soil samples (0–15 cm) were extracted with ion-exchange resins, extracts were analysed by inductively coupled plasma methods (ICP), and the results were regressed against seed yield using SAS PROC STEPWISE analysis using forward selection, backward elimination and maximum R² routines. The seed yield of each variety showed a correlation with a unique set of resin-extractable concentrations of V, phosphorus (P), Mg and Ca, and the V:(V + P), Mg:(Mg + Ca), Mg:(Mg + 1000 V) and Ca:(Ca + 1000 V) ratios. Variety 9091 was most sensitive to the Mg:(Mg + Ca) ratio. Variety 9061 was most sensitive to extractable V and to the V:(V + P) ratio. Variety 704 was sensitive to extractable P, V and Ca and the Mg:(Mg + 1000 V) ratio. For variety 9091, Mg fertilization (not currently practised) may be an economical practice, whereas P fertilization of 704 may not be economical. Each regression technique varied slightly in identification of important factors in seed yield. Concentrations and ratios of resin-extractable elements in soil provide insights into optimal genotype selection and possible management alternatives for a given soil.     

Evaluation of two N cycle models for the prediction of N mineralization from grassland soils in the UK

Abstract. The ability of two nitrogen cycle models, of contrasting complexity, to predict N mineralization from a range of grassland soils in the UK, was evaluated. These were NCYCLE, a simple mass balance model of the N cycle in UK grasslands, and CENTURY, a more complex model simulating long-term C, N, P & S dynamics in grassland ecosystems. The models were tested using field measurements of net N mineralization from a range of grassland soils (differing in soil type, history & management practice), obtained over a 2 year period using a soil core incubation technique. This method was considered to measure the total net release of mineral N from the soil organic matter over a specified time, including N which may have been recycled several times. NCYCLE consistently under-estimated mineralization rates at all sites. By contrast, there was some correlation between CENTURY predictions of net N mineralization and field measurements. This may have reflected the different abilities of the two models to simulate N recycling. Neither model, however, was able to predict adequately the effect of cultivation and reseeding on net N mineralization.     

Acupuncture for general veterinary practice.

Since the International Society of Veterinary Acupuncture (IVAS) was founded in 1974, acupuncture (AP) has received greater acceptance by veterinary professionals throughout the world. This article introduces some important animal diseases that respond well to AP therapy. These include resuscitation of small animals, treatment of anoestrous gilts and sows, bovine reproductive disease, canine vertebral problems and equine backpain, etc. Conventional medicine considers these to be difficult cases to treat. Veterinarians have become more aware of the benefits of AP especially for those diseases, thanks to the efforts of experienced practitioners and scientists, and the many published reports on veterinary AP that have introduced some good indications for AP therapy in veterinary practice. Possible mechanisms behind the effectiveness of AP are discussed. This article aims to introduce veterinarians to good indications for AP to initiate their interest in the practice of AP. Although this is a rapidly expanding field, a long march must begin with one step. We wish this article to be the shoes for such a march. For more information on veterinary AP, contact IVAS , P.O. Box 1478, Longmont, CO.. 80502, USA (http://www.ivas.org), or your national veterinary acupuncture society (http://www.komvet.at/ ivadkom/vapsocs.htm).     

Mid-storage and pre-sowing seed treatments for lettuce and carrot

Mid-storage hydration-dehydration treatments with medium-vigour lettuce seed very effectively reduced physiological deterioration in storage and ultimately showed better field emergence and final yield. Soaking-drying treatment of high-vigour lettuce seed was not effective. Pre-sowing treatment of medium-vigour lettuce seed, though less effective than mid-storage treatment, gave better field performance, but high-vigour seed did not benefit from the treatment. For carrot, both mid-storage and pre-sowing seed treatments proved beneficial, with the former showing better performance than the latter. Unlike lettuce, well-preserved carrot seed responded positively to the pre-sowing treatment.     

The extent of genetic diversity among Vanilla species: Comparative results for RAPD and ISSR

Vanilla is a large genus of about 110 species in the orchid family (Orchidaceae), including the species Vanilla planifolia from which commercial vanilla flavoring is derived. Since most species of vanilla are considered rare and endangered there is an urgent need to conserve them through genetic analysis and propagation/conservation studies on this crop.The present study investigated the genetic diversity among nine leafy- and leaf-less Vanilla species employing 30 decamer RAPD primers and 10 ISSR primers. The species under study were diverse and displayed a range of variability (0–66% and 0–81% for RAPD and ISSR, respectively). A total of 154 RAPD polymorphic markers (83.24%, h = 0.378) and 93 ISSR polymorphic markers (86.11%, h = 0.363) were used to generate a genetic similarity matrix followed by the cluster analysis. Specific groupings were revealed by each cluster analysis with slight variation between two different markers. Among the nine species studied, V. planifolia, Vanilla aphylla and Vanilla tahitensis revealed very low level of variation within their collections, thus indicating a narrow genetic base. The large genetic distance of Vanilla andamanica from other species suggests its different origin. A close genetic affinity was observed between the pairs V. planifolia, V. tahitensis and Vanilla albida, V. aphylla. These are the first comparative results for RAPD and ISSR reporting inter-relationship among nine cultivated, wild and hybrid Vanilla species.     

明清时期对烟草药用价值的认识和利用

烟草原产美洲,又名"菸"、姆巴古、淡巴菰、淡巴姑、大古、淡肉果、担不归等,别名"相思草"、"返魂烟"、"气死名医草",明代万历年间传入中国.烟草传入中国后,传种速度很快.明末的谈迁曾描述当时烟草的种植情形时说:"今艺及江南、北"(1)(中集《荣植·金丝烟》).