全文获取类型
收费全文 | 262335篇 |
免费 | 15825篇 |
国内免费 | 7850篇 |
专业分类
林业 | 30260篇 |
农学 | 24529篇 |
基础科学 | 8880篇 |
50068篇 | |
综合类 | 27174篇 |
农作物 | 19899篇 |
水产渔业 | 17202篇 |
畜牧兽医 | 73623篇 |
园艺 | 7984篇 |
植物保护 | 26391篇 |
出版年
2022年 | 2882篇 |
2021年 | 4778篇 |
2020年 | 5018篇 |
2019年 | 5626篇 |
2018年 | 5034篇 |
2017年 | 6017篇 |
2016年 | 6579篇 |
2015年 | 6816篇 |
2014年 | 8012篇 |
2013年 | 19183篇 |
2012年 | 8518篇 |
2011年 | 10288篇 |
2010年 | 10551篇 |
2009年 | 10909篇 |
2008年 | 9060篇 |
2007年 | 8410篇 |
2006年 | 9040篇 |
2005年 | 7963篇 |
2004年 | 6121篇 |
2003年 | 5686篇 |
2002年 | 4756篇 |
2001年 | 5008篇 |
2000年 | 5047篇 |
1999年 | 5498篇 |
1998年 | 5502篇 |
1997年 | 5331篇 |
1996年 | 4976篇 |
1995年 | 5405篇 |
1994年 | 4658篇 |
1993年 | 4305篇 |
1992年 | 3852篇 |
1991年 | 3819篇 |
1990年 | 3443篇 |
1989年 | 3236篇 |
1988年 | 2804篇 |
1987年 | 2755篇 |
1986年 | 2503篇 |
1985年 | 2872篇 |
1984年 | 2866篇 |
1983年 | 2805篇 |
1982年 | 2510篇 |
1981年 | 2491篇 |
1980年 | 2467篇 |
1979年 | 2357篇 |
1978年 | 2256篇 |
1977年 | 2169篇 |
1976年 | 2014篇 |
1975年 | 1756篇 |
1974年 | 1756篇 |
1973年 | 1696篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
31.
Ruimin Chao Chengqiang Xia Caixia Pei Wenjie Huo Qiang Liu Chunxiang Zhang Youshe Ren 《Journal of animal physiology and animal nutrition》2021,105(1):26-34
The objective of this study was to investigate the characteristics of ruminal microbial communities of alpacas (Lama pacos) and sheep (Ovis aries) fed three diets with varying ratios of roughage (corn stalk) to concentrate, 3:7 (LS), 5:5 (MS) and 7:3 (HS). Six alpacas (one-year-old and weighing 29.5 ± 7.1 kg) and six sheep (one-year-old and weighing 27.9 ± 2.7 kg) were used in this study, in a replicated 3 × 3 Latin square experiment. Total protozoa concentration was determined under the microscope; total fungi and methanogens were assessed using quantitative polymerase chain reaction and expressed as a percentage of total bacterial 16S rRNA gene copies; bacterial communities were investigated by targeted 16S rRNA gene (V3–V4 region) sequencing. The percentage of fungi was significantly higher in alpacas than in sheep under the LS diet, while the concentration of protozoa was significantly lower in alpacas under HS, MS and LS diets. The alpha diversity including Shannon, Chao l and ACE indices of bacterial communities was higher in alpacas than in sheep, under the LS diet. A total of 299 genera belonging to 22 phyla were observed in the forestomach of alpaca and sheep, with Bacteroidetes and Firmicutes dominating both animal species. Phyla Armatimonadetes and Fusobacteria, as well as 64 genera, were detected only in alpacas, whereas phyla Acidobacteria and Nitrospira, as well as 44 genera, were found only in sheep. The abundance of cellulolytic bacteria, including Butyrivibrio and Pseudobutyrivibrio, was higher in alpacas than in sheep under all three diets. These differences in the forestomach microbial communities partly explained why alpacas displayed a higher poor-quality roughage digestibility, and a lower methane production. Results also revealed that the adverse effects of high-concentrate diets (70%) were lesser in alpacas than in sheep. 相似文献
32.
Sepsis of the calcaneal bursae (CB) presents significant treatment challenges with limited clinical data available in the literature. The objective of this retrospective cohort study was to assess the clinical outcomes associated with CB lavage using either a through-and-through needle or bursoscopic technique. Clinical records of 29 horses treated for septic calcaneal bursitis using either technique between 2005 and 2019 were reviewed. Fisher’s exact test was used to assess statistical significance between first surgical technique and success at first surgery (i.e. not requiring >1 lavage), survival to discharge and return to work (RTW). Bursoscopy was performed in 13/29 (44.8%) cases, and needle lavage in 16/29 (55.2%). In the needle group, 12 (75%) horses were discharged following the first surgery. Four had repeat interventions; two (12.5%) had needle lavage and two (12.5%) had bursoscopy. Of the two horses to have repeat needle lavage, one was subjected to euthanasia and one discharged, and of the two that underwent bursoscopy, one was discharged and one received a third bursoscopy prior to discharge. In the bursoscopy group, seven (53.8%) were discharged and three (23.1%) were subjected to euthanasia following the first surgery. Three (23.1%) received a second bursoscopic lavage with one discharged, one subjected to euthanasia and one having a third bursoscopic lavage prior to discharge. Overall, 18/24 (75%) followed up cases RTW, 10 (55.5%) from the needle group, eight (44.4%) the bursoscopy group. No statistically significant differences between first surgical technique used and success at first surgery (no subsequent lavage(s) required), survival to discharge or return to work were detected. The main limitations of this study are that it is a retrospective study, has a small population with limited statistical power and potential selection bias. No statistically significant differences existed between the outcomes of the two techniques, contrary to the belief that bursoscopic lavage is superior. Larger, multicentred studies, with greater statistical power are required to further assess this relationship. 相似文献
33.
Wang Zhun Zhang Hengheng Dong Qiang Gui Huiping Wang Xiangru Pang Nianchang Li Yongnian Niu Jing Jin Dingsha Wang Sujie Zhang Xiling Song Meizhen 《棉花学报》2021,32(6):538-551
[Objective] The aim of this study is to evaluate nitrogen efficient cotton germplasms and improve nitrogen use efficiency. [Method] Eighty cotton germplasms were selected and evaluated in the hydroponic experiment under low (0.25 mmol·L-1) and high (5 mmol·L-1) nitrogen concentration. Different traits for screening were identified and nitrogen use efficiency types were classified. Field experiments were also performed for comparison and confirmation of the identified germplasms. [Result] The results showed that there were significant differences in the total plant dry matter, shoot nitrogen accumulation and nitrogen absorption efficiency in cotton germplasms at the two nitrogen levels. Based on coefficient of variation, principal component analysis and correlation, six traits including total plant dry matter, shoot dry matter, root dry matter, total nitrogen accumulation, shoot nitrogen accumulation and nitrogen absorption efficiency were used as screening indicators. According to the Heatmap clustering analysis and the nitrogen efficiency comprehensive index, two germplasms (Lu05R59 and CCRI 69) were identified as low nitrogen tolerant and nitrogen efficient, and two germplasms (Coker 201 and Xinluzhong 30) as low nitrogen sensitive and nitrogen inefficient. The results of field experiment were consistent with the results of the hydroponic culture at the seedling stage. [Conclusion] It was finally determined that Lu05R59 and CCRI 69 were the low nitrogen tolerant and nitrogen efficient germplasms, and Coker 201 and Xinluzhong 30 were low nitrogen sensitive and nitrogen inefficient germplasms. The results of these studies provide the possibility for screening and rapid identification of nitrogen use efficiency in cotton at the seedling stage, and provide the ideal materials and theoretical basis for further study of cotton nitrogen efficient. 相似文献
34.
Sonia Czarnes Pierre-Edouard Mercier Damien G. Lemoine Jihane Hamzaoui Laurent Legendre 《Journal of Agronomy and Crop Science》2020,206(5):505-516
Members of the bacterial genus Azospirillum are root-associated bacteria that increase yield in cereals by promoting growth and alleviating drought stress. How plants integrate the many bacterium-derived growth-promoting stimuli with other environmental factors to generate a coordinated response remains unresolved. Using a commercial Azospirillum strain, A. lipoferum CRT1 and two host maize cultivars, it was observed that bacterization reduced the drought-induced increase in lateral root growth and enhanced the flood-induced increase in lateral root growth in the more drought- and flood-sensitive cultivar. In the other one, A. lipoferum CRT1 only elicited a moderate root growth response under low soil water potential. The photosynthetic potential and activity were increased in the earlier cultivar and decreased in the later one, irrespective of the soil water content. No impact of the bacterium was seen on the growth of the leaves of both cultivars under both stresses until the third leaf stage, therefore suggesting that it is a consequence of multiple primary adaptations to biotic and abiotic stresses. It is suggested that host–bacteria recognition leads to a stress-specific modulation of the root response and a differential stress-independent effect on photosynthesis. This is the first report of the impact of Azospirillum under flood conditions. 相似文献
35.
Ting Shi Yan-Yan Yu Jia-Jia Dai Yi-Ting Zhang Wen-Peng Hu Li Zheng Da-Yong Shi 《Marine drugs》2021,19(3)
The species Pseudogymnoascus is known as a psychrophilic pathogenic fungus with a ubiquitous distribution in Antarctica. Meanwhile, the study of its secondary metabolites is infrequent. Systematic research of the metabolites of the fungus Pseudogymnoascus sp. HSX2#-11, guided by the method of molecular networking, led to the isolation of one novel polyketide, pseudophenone A (1), along with six known analogs (2–7). The structure of the new compound was elucidated by extensive spectroscopic investigation and single-crystal X-ray diffraction. Pseudophenone A (1) is a dimer of diphenyl ketone and diphenyl ether, and there is only one analog of 1 to the best of our knowledge. Compounds 1 and 2 exhibited antibacterial activities against a panel of strains. This is the first time to use molecular networking to study the metabolic profiles of Antarctica fungi. 相似文献
36.
The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy. 相似文献
37.
为了丰富紫花槭转录组数据,进一步开展紫花槭秋季叶片呈色机制研究.本研究以紫花槭秋季转色期三个阶段(前期,中期,后期)叶片为材料,采用高通量测序技术进行转录组初步分析.转录组数据共获得50501条Unigene,有35316条Unigene在数据库中得到注释,其中NR数据库中注释到的Unigene数量最多,共35024条,占69.4%.在注释到的物种中,紫花槭比对的Unigene与甜橙(Citrus sinensis)相似度最高,共有4290条,占12.25%.紫花槭转录组中的Unigene根据GO功能可分为生物学过程、细胞组分和分子功能3大类,共有25375条,其中生物学过程的基因最多,主要聚集于代谢过程和细胞过程等.基于Unigene库的基因结构分析,其中SSR分析共获得12711个SSR标记,占Unigene总数的36%.SSR位点共包含150种重复基元,单碱基重复所占比例最高(7184个,61.86%),四碱基重复、五碱基重复和六碱基重复所占比例较低.Unigene库中共有328239个SNP位点,发生频率为1/190 bp,SNP位点分为转换和颠换两种类型的碱基替换方式,其中碱基转换位点213787个(65.13%),碱基颠换位点114452个(34.87%),碱基转换类型发生频率高于颠换类型.6种单碱基变异中,2种转换类型A/G、C/T的发生频率分别为33.03%和32.10%;4种颠换类型中A/T发生频率最高,为11.52%;C/G发生频率最低,为5.79%.紫花槭转录组秋季叶色表达的转录组分析,可为紫花槭叶色基因调控、定向分子育种和培育彩叶新品种提供研究提供基础的数据信息. 相似文献
38.
39.
Sirirat Chatvijitkul Claude E. Boyd D. Allen Davis Aaron A. McNevin 《Journal of the World Aquaculture Society》2017,48(1):7-19
Global averages were obtained for amounts of energy, land, water, wildfish, nitrogen, and phosphorus embodied in aquaculture feed ingredients. These data allowed amounts of these embodied resources to be calculated for typical feed formulations for channel catfish, Ictalurus punctatus; hybrid catfish, I. punctatus♀ × I. furcatus♂; Vietnamese catfish, Pangasius spp.; Atlantic salmon, Salmo salar; rainbow trout, Oncorhynchus mykiss; tilapia, Oreochromis spp.; whiteleg shrimp, Litopenaeus vannamei; and black tiger shrimp, Penaeus monodon. Embodied resource use per m.t. of feed varied among species: energy, 4.90–12.48 GJ/m.t.; land, 0.082–0.312 ha/m.t.; water, 502–1227 m3/m.t.; wildfish, 0–2880 kg/m.t.; nitrogen, 3.08–8.63 kg/m.t.; phosphorus, 1.16–5.62 kg/m.t. These calculations did not account for variations in site‐specific factors related to embodied resources and feed composition and use. But they suggest that reducing feed conversion ratio (FCR) by 0.1 unit for the seven species (species groups) could potentially reduce feed use by around 1.1 million tonne (Mt) while conserving 9.8 million GJ of energy, 270,000 ha of agricultural land, 1.4 billion m3 of freshwater, and 1.24 Mt of wildfish. Reduction of the FCR is a powerful means of lessening farm‐level production costs and negative impacts of feed production and use. 相似文献
40.