In vivo kinetic study on uptake and distribution of intramuscular tritium-labeled polysulfated glycosaminoglycan in equine body fluid compartments and articular cartilage in an osteochondrial defect model

The uptake and distribution of intramuscularly (IM) administered tritium-labeled polysulfated glycosaminoglycan (³H-PSGAG) in serum, synovial fluid, and articular cartilage of eight horses was quantitated, and hyaluronic acid (HA) concentration of the middle carpal joint was evaluated in a pharmacokinetic study. A full-thickness articular cartilage defect, created on the distal articular surface of the left radial carpal bone of each horse served as an osteochondral defect model. ³H-PSGAG (500 mg) was injected IM, between 14 and 35 days after creation of the defects. Scintillation analysis of serum and synovial fluid, collected from both middle carpal joints at specific predetermined times up to 96 hours post-injection, revealed mean ³H-PSGAG concentrations peaked at 2 hours post-injection. ³H-PSGAG was detected in cartilage and subchondral bone 96 hours post-injection in samples from all eight horses. There were no statistically significant differences in ³H-PSGAG concentration of synovial fluid or cartilage between cartilage defect and control (right middle carpal) joints.

HA assay of synovial fluid revealed concentrations significantly increased at 24, 48, and 96 hours post-injection in both joints. The concentration nearly doubled 48 hours post-injection. However, no statistically significant differences were found between synovial concentrations of HA in cartilage defect and control joints.

³H-PSGAG administered IM to horses, was distributed in the blood, synovial fluid, and articular cartilage. HA concentrations in synovial fluid increased after IM administration of polysulfated glycosaminoglycan.     

赖氨酸占蛋白质及饲粮的比例对杜洛克猪生长肥育性能及胴体品质的影响

杜洛克猪48头,体重27kg左右,分6组。试验前期(27～60kg)日粮的消化能均为13.67MJ)/kg,后期(60～90kg)均为13.44MJ/kg。粗蛋白1、2、3组前期均为18％,后期均为16％;4、5、6组前期均为16％,后期均为14％。同时配合3种赖蛋比水平6.6～6.9％,6.1～6.25％及5％。试验结果表明:4、5、6组平均日增重、饲料利用率及胴体瘦肉率都略高于1、2、3组,但差异不显著(P>0.05)。在前期,以第5组平均日增重最高(达782克),极显著高于其他各组(P<0.01);饲料利用率2.41,极显著好于其他各组(P<0.01)。后期以第5组平均日增重最高(875克)。试验前期,第5组获得最佳的日增重(822克),极显著高于其他各组(P<0.01),饲料利用率2.98,胴体瘦肉率65.79％,与其他各组差异不显著(P>0.05)。在两种粗蛋白水平组内,均以赖蛋比6.1～6.25％的第5组和第2组生长速度最快(822克和778克)。说明在相同的能量水平下,赖蛋比是影响生长速度的主要因素。     

在桩基础设计中两种规范的差异比较--《建筑地基基础设计规范》与《建筑桩基技术规范》

通过对二部规范的对比，阐明“地基基础”与“桩基”相比在桩基础设计中作了较大的调整，这样既节省了工程造价又提高了桩基础设计质量。     

巴氏杀菌牛乳和UHT牛乳的差别

研究了巴氏杀菌、超高温灭菌对牛乳中各种成分的影响，结果发现此两种热处理方式对牛乳中蛋白质、维生素、矿物质和产品风味产生不同影响，而对脂肪的影响不大。     

海涂实行草地农业的综合生态学方法

依据海涂独特的自然属性确定开发利用方案是保证土地资源持续生产的关键。采用降低土壤盐分、实行保护性耕作、用植物残余物覆盖地面、种植牧草防止土壤侵蚀和促使生草层土的发育，保证了以潜在形式集中的富含必需营养元素的腐殖质在土壤中聚积，改善土壤结构，提高土壤水分和养分的利用效率，并以牧草饲养家畜的土地经营活动形成了成功的草地农业生产。     

Comments on the method to test side effects of pesticides on the predatory mitePhytoseiulus persimilis Ath.-Hen

A paper published byKniehase & Zoebelein (1990) describes a new laboratory method to test the side effects of pesticides on the predatory mitePhytoseiulus persimilis and criticizes the laboratory test developed by the International Working Group pesticides and beneficial organisms of the International Organization for Biological Control (IOBC), West Palaearctic Regional Section (WPRS). The present publication discusses the advantages and disadvantages of both methods, responds to the criticism and mention the overall concept of the IOBC/WPRS Working Group including semifield and field test methods.In einer Arbeit vonKniehase undZoebelein (1990) wird ein neues Laborverfahren zur Prüfung der Nebenwirkung von Pflanzenschutzmitteln auf die RaubmilbePhytoseiulus persimilis beschrieben. Die genannten Autoren äußern sich kritisch über das seither von der Arbeitsgruppe Pflanzenschutzmittel und Nutzorganismen der International Organization for Biological Control (IOBC), West Palaearctic Regional Section (WPRS) praktizierte Verfahren. In der vorliegenden Arbeit werden die Vor- und Nachteile der beiden Prüfmethoden diskutiert. Einer Reaktion auf die Kritik folgt eine kurze Beschreibung der gesamten Konzeption der genannten Arbeitsgruppe, die auf einer Kombination aus Labor-, Halbfreiland- und Feldprüfverfahren beruht.     

海南油棕果腐病的病原鉴定及发病条件的研究

三年来作者对于幼龄結果油棕的果穗和果实腐烂的症状、分布、蔓延为害,其发生条件和栽培管理的关系进行了詳細的調查和观察,并进行了病原分离、培养、田间人工接种和試探性化学保护等試驗。同时对果实离层組織的形成进行了切片检查。 結果指出海南十二个地区的幼龄结实棕园普遍出現的花、果、穗腐与环境条件和栽培管理有密切关系。果腐是果实离体后从蒂部組織开始的。从腐果組織中經常可以分离到細菌、炭疽菌和鐮刀菌。多次田間接种証明这些菌对健康果实和果穗均无致病能力。大田喷药无效。看来,油棕果腐病是由于环境坏、管理差的条件下,未成熟或接近成熟的果实产生离层而与果柄分离,再由外界杂菌腐食脫果而致腐烂。可見,本病是屬于非侵染性的生理病害。