Verformungsverhalten künstlicher Makroporen unter variierenden Druck‐ und Bodenfeuchtebedingungen

Structural deformation of artificial macropores under varying load and soil moisture In the present study, the stability and deformation behavior of artificial macropores under varying load and soil moisture levels was investigated by means of X‐ray computed tomography (CT). The results should be a reference for similar studies on soil samples from field trials. The soil tested was a well structured humic silt loam with a bulk density of 1 g cm^—3. Round‐shaped pores of vertical and 45 degree angle orientation were drilled into the samples with a plastic needle (∅︁ 5 mm). These samples were compacted in an uniaxial compression device at four different moisture levels and four pressure stages each. Stepwise CT imaging and its 3‐dimensional reconstruction enabled us to study systematically the mode and intensity of pore deformation. As a result four different deformation stages could be identified in dependence from load, soil moisture, and pore orientation. The deformation stage ”︁stable” was characterized by mostly unaffected pore dimensions and shapes. Increasing load and/or moisture content led to prominent bottle necks within the pores which was named ”︁structure deformation”. Due to the shape and size of these bottle necks it seems to be most likely that still intact aggregates were moved into the inner pore space, reducing the mean cross sectional areas. The deformation stage ”︁total deformation” appeared with further increase of load and/or moisture. The aggregated structure disappeared while the inner roughness of the pores became smoother again. This represents a viscoplastic deformation. Cross sectional areas, pore lengths, and volumes significantly decreased. The stage ”︁extinction” was finally reached at water contents around the liquid limit, where the pore structure was completely lost, at least on CT resolution level. The deformation stages could be attributed to load stages depending from pore orientation. Unexpectedly, all pores kept their originally round shape over all stages until extinction.     

Prof. Dr. SC. Gisela Höflich zum 65 geburtstag

Auch die K‐Werte im Unterboden sind primär durch den unterschiedlichen Tongehalt der Versuchsböden determiniert. Durch differenzierte K‐Düngung verursachte negative bzw. positive K‐Bilanzen führen im Unterboden zu geringeren Veränderungen der K‐Werte als im Oberboden: Der K_t‐Gehalt wird durch K‐Düngung im Mittel der drei Dauerversuche gegenüber K‐Mangel in der Krume um 36 mg/100 g, im Unterboden um 14 mg/100 g erhöht. Dieser Unterschied widerspiegelt sich in allen anderen Werten des K‐Status (K_HCI, K_e, K_w, K_DL) und der K‐Dynamik (K‐Sorption, K‐Desorption, K‐Fixierung). Die relative Veränderung der K‐Kennwerte nimmt mit steigendem Tongehalt ab und ist am deutlichsten bei verschiedenen Werten des labilen Kaliums. Das Verhältnis des K_DL zur Summe des wasserlöslichen plus austauschbaren Kaliums beträgt im Unterboden der K‐Mangelvarianten 34% in Lauchstädt, 56% in Halle und 136% in Thyrow; die entsprechenden Werte in den Volldüngungsvarianten betragen 40, 61 und 200%.     

Complement of integrated fertilizer management and integrated pest management concepts to ameliorate faba bean growth and yield

Twenty-one microbial preparations recommended for controlling pathogenic fungal strains causing root rot and wilt diseases of faba bean were investigated for antibiosis against several symbiotic and associative diazotrophs adopting a modified agar-plate-inhibition-zone assay. Rhizobium and Bradyrhizobium exhibited a somewhat similar susceptibility to biocontrol agents while associative diazotrophs showed variable responses. Azotobacter, compared to others, was severely inhibited by such bio-candidates. The members of the biofertilizer formulation ‘Biofertan’ did bear mixed cultivation with the majority of biocontrol agents. Among those, Bacillus subtilis was deemed the pioneer. In pot experiments, almost all the antagonists significantly restricted the severity of root rot and wilt diseases besides modifying faba bean seedling stand and improving plant development. This was very obvious with shoot biomass increases of >?50%. Moreover, the bioagents successfully recovered the legume establishment, seriously injured due to pathogenic fungal infection. Simultaneous inoculation with Rhizobium and biocontrol agents provided more growth stimulation compared to either when introduced individually. Field-grown faba beans were inoculated with the diazotroph and representatives of biocontrol strains by two different methods, seed coating and over-head soil. Growth parameters determined were the highest when the legume plant was seed-coated by Rhizobium simultaneously over-head soil inoculated with the bacterial bioagents; this was reported with Bacillus subtilis and Pseudomonas aerugenosa. The significance of combined application of biofertilizer and bioagent to ensure cheap, clean and safe farm products is discussed.     

A comparison of lime requirements by five methods on grassland mineral soils in Ireland

Liming is necessary for good nutrient availability and crop growth. Lime use in Ireland is now the lowest in half a century. A recent study shows that grassland mineral soils in Ireland has a mean pH of 5.4 and mean lime requirement (LR) of 9.3 t/ha ground limestone. There have been a number of studies in the USA to re-evaluate LR, but little activity in the European Union (EU) in recent years. The primary aim of our research was to compare five methods for estimating LR, which included the Shoemaker–McLean–Pratt (SMP) buffer method currently used in Ireland (IRL), the Sikora buffer method used at the University of Kentucky (UKY), Ca(OH)₂ titration used at University of Georgia (UGA), the modified Mehlich buffer method used at Penn State University (PSU) and the UK RothLime model, using 57 representative grassland mineral soils from Ireland with a pH range from 4.8 to 6.6. The secondary aim was to explore an alternative to the SMP buffer that does not involve the use of toxic chemicals. The results show good agreement between the pH measured by the Irish and three US laboratories and reasonably good agreement in LR estimated by five methods. The main conclusions are: (1) a significant proportion of grassland on mineral soils in Ireland would benefit from liming to increase soil pH, (2) on average, LRs as recommended in Ireland are higher than those advised elsewhere , ( 3) the target pH in Ireland is high compared with that in other countries and should be reduced from pH 6.5 to 6.2, (4) the SMP buffer method should be replaced by a suitable alternative and, in principle, any of the four methods studied would be suitable, (5) to find the most suitable alternative for accurate LR advice it would be necessary to compare the different methods to the actual LR from incubation of representative soils with calcium hydroxide.     

Discovering Transgenic Elite Events: Using Information from Early Screening Trials for Improving Experimental Design

The transgenic improvement of a commercial crop requires a suitable transgene as well as a suitable insertion location in the crop’s genome. An elite event is a successful insertion of an active transgene, and many events need to be screened to detect such elite events. This is expensive, hence optimization is needed.     

Resistance of soybean vegetative storage proteins (S-VSPs) to proteolysis by rumen microorganisms

Soybean vegetative storage proteins (S-VSPs) are lysine-rich and, hence, are potentially of high nutritive value for high productive ruminants. Using S-VSPs from wild-type soybean and from transgenic tobacco plants expressing either one of the two S-VSPs subunits (S-VSP alpha or S-VSP beta) or both, we tested their stability in cow rumen fluid under in situ conditions, using SDS-polyacrylamide gel electrophoresis. Proteolysis and degradation pattern of S-VSPs from transgenic tobacco leaves occurred relatively fast compared with that of wild-type (WT) soybean plants. Comparing the two S-VSPs subunits expressed in transgenic plants, we found that S-VSP alpha was degraded much faster than S-VSP beta. The degradation pattern of S-VSPs in transgenic tobacco plants expressing both subunits resembled that of WT soybean. In contrast, the degradation pattern of transgenic tobacco plants expressing a single subunit was different. These finding suggest that the quaternary structure of S-VSPs may be an important factor determining their resistance to rumen degradation. Our results also suggest that the stability to rumen proteolysis of a given protein, when expressed in a transgenic plant, may not always be predictable and has to be verified.     

GIS支持下1985-2000年北京林地数量、质量演变与驱动分析

在收集、研究1985年、1995年、2000年北京土地覆盖类型的图像和矢量数据的基础上,提取不同时间段北京林地相关的数据信息,并从多个角度分析研究区内各类林地与其它土地覆盖类型之问相互转化和各类林地之间的演替情况,得出2000年北京各类林地面积总量与1985年相比有少量增加,但林地总体质量水平却有下降趋势;并从经济社会发展以及国家及北京的生态环保政策等方面,对林地演变的驱动因子进行分析.     

Strong seasonal variations in net ecosystem CO2 exchange of a tropical pasture and afforestation in Panama

Pasture and afforestation are land-use types of major importance in the tropics, yet, most flux tower studies have been conducted in mature tropical forests. As deforestation in the tropics is expected to continue, it is critical to improve our understanding of alternative land-use types, and the impact of interactions between land use and climate on ecosystem carbon dynamics. Thus, we measured net ecosystem CO₂ fluxes of a pasture and an adjacent tropical afforestation (native tree species plantation) in Sardinilla, Panama from 2007 to 2009. The objectives of our paired site study were: (1) to assess seasonal and inter-annual variations in net ecosystem CO₂ exchange (NEE) of pasture and afforestation, (2) to identify the environmental controls of net ecosystem CO₂ fluxes, and (3) to constrain eddy covariance derived total ecosystem respiration (TER) with chamber-based soil respiration (R_Soil) measurements. We observed distinct seasonal variations in NEE that were more pronounced in the pasture compared to the afforestation, reflecting changes in plant and microbial activities. The land conversion from pasture to afforestation increased the potential for carbon uptake by trees vs. grasses throughout most of the year. R_Soil contributed about 50% to TER, with only small differences between ecosystems or seasons. Radiation and soil moisture were the main environmental controls of CO₂ fluxes while temperature had no effect on NEE. The pasture ecosystem was more strongly affected by soil water limitations during the dry season, probably due to the shallower root system of grasses compared to trees. Thus, it seems likely that predicted increases in precipitation variability will impact seasonal variations of CO₂ fluxes in Central Panama, in particular of pasture ecosystems.     

Analysing flow patterns from dye tracer experiments in a forest soil using extreme value statistics

Preferential flow of water in soil is now recognized as a common phenomenon. It results in complex flow patterns that can be visualized by dye tracers and increases the risk of pollutants reaching greater depths. We analysed the behaviour of a risk index for vertical solute propagation based on extreme value theory. This risk index can be calculated from binary images of dye‐stained soil profiles and is defined as the form parameter of the generalized Pareto distribution. We did five tracer experiments with Brilliant Blue and iodide under changing initial (variable initial soil moisture) and experimental conditions (different irrigation rates). Our results indicate some persistence of the risk index against small changes of experimental conditions such as the irrigation rate. On the other hand, it seems to be affected by initial soil moisture. Comparisons of Brilliant Blue and iodide patterns show that the form parameter alone is not sufficient to estimate the risk of vertical solute propagation. Therefore we propose to combine the risk index with the scale parameter of the generalized Pareto distribution.