Analysis of fat-soluble vitamins. XVI. Antirachitic activity of 5,6-trans-vitamin D3 alone and in the presence of 5,6-cis-vitamin D3 resin, using chick bioassays.

Two biological assays were conducted in which the antirachitic activity in chicks of 5,6-trans-vitamin D3 added to feed is compared with that of 5,6-cis-vitamin D3. On the basis of the results obtained it is concluded that the relative potency of the trans isomer is, at the most, 5% and that the antirachitic activity of the trans isomer is not markedly enhanced (an increase to a relative potency of 16%, at the most) if the cis isomer is also included in the diet. The results are not conclusive on the inhibition or lack of inhibition of the antirachitic activity of 5,6-cis-vitamin D3 by the presence of 5,6-trans-vitamin D3 in the feed.     

Gas-liquid chromatographic determination of residues of methanesulfonate of n-aminobenzoic acid ethyl ester in fish.

In this gas-liquid chromatographic procedure for determining residues of methanesulfonate of m-aminobenzoic acid ethyl ester (MS-222) in fish muscle, homogenized tissue is extracted with distilled water, and proteins are removed by coagulation with trichloroacetic acid, centrifugation, and filtration. After careful pH adjustment of the filtrate, MS-222 is partitioned into benzene-ethyl ether and measured by alkali flame ionization gas chromatography. Tissues with known additions of 1-19 microgram MS-222/g were analyzed, with recoveries of 84-95%.     

The effect of urea on the voluntary intake by calves of silages preserved with formaldehyde and formic acid

Perennial ryegrass cv. S23 was preserved by fermentation (C), or with the addition of 8.7 1 formalin (35% w/w formaldehyde) per t fresh herbage (F) or of 9.0 1 equal mixture (by vol.) of formalin and formic acid (85% w/w solution) per t fresh herbage (FF). These three silages comprised the treatments in a three-period, crossover design in which urea (u) was given at the rate of 20 g per kg dietary DM to half the animals. Twelve calves were allocated to the treatment sequences when they were approximately 12 weeks of age from within groups which had previously received diets with or without urea. Calves ate significantly (P< 0.001) less of silages F or FF than of silage C when each was offered alone, but when urea was given, intakes of silages F and FF were significantly (P < 0.001) increased such that the intakes of all three silages with urea were similar (23.6, 19.6, 19.3, 24.2, 24.2, 24.7 ± 0.51 g DM per kg LW for C, F, FF, Cu, Fu and FFu, respectively). Significantly (P<0.01) more of the DM in silage C was digested than in F or FF and the addition of urea significantly (P<0.05) increased the digestibility of DM from silage FF only (73.8, 71.3, 69.8, 74.6, 70.7, 72.5 ± 0.72% for C, F, FF, Cu, Fu and FFu, respectively). When the silages were given alone, calves spent longer eating and ruminating per kg DM ingested with F or FF than with C. The time spent on F and FF was significantly (P<0.01) reduced by the addition of urea (303, 388, 411, 297, 299, 290 ± 18.9 min per kg DM ingested for C, F, FF, Cu and Fu and FFu, respectively). The results presented suggest that urea partially alleviated the reduction in voluntary intake by calves given herbage preserved using formalin, but the mode of action was not clear.     

Silage and milk production: a comparison between four rates of groundnut cake supplementation of silage of high digestibility

Grass silage made in late May from S24 perennial ryegrass was offered to twelve Ayrshire cows in a 16-week feeding experiment. The silage had a DM content of 25.3%, a pH of 3.91, and contained 16.8% CP and 68.4% digestible organic matter in the DM. The silage was fed ad libitum and was the sole feed in the control treatment. In the other three treatments the silage was supplemented with a cube offered at rates of 0.8, 1.4 and 2.0 kg per 10 kg milk. The cube contained 82.2% groundnut, plus molasses and minerals, and had 37.9% CP in the DM. The daily intake of silage DM was 11.4 kg per cow on average over the four treatments which did not differ significantly. The mean daily milk yields were 14.8 kg per cow in the control treatment, and 16.5, 18.2 and 18.4 kg in the 0.8, 1.4 and 2.0 kg supplement treatments respectively. The SNF, CP and lactose contents of the milk were Wghest on the 1.4 kg supplement treatment. It is concluded that with a high digestibility silage, there is little evidence to support the feeding of more than 1.4 kg of high-protein cubes per 10 kg milk in the declining phase of lactation.     

INTERACTIONS BETWEEN FIRST AND SECOND APPLICATIONS OF FERTILIZER NITROGEN AND IMPLICATIONS IN EFFICIENCY OF NITROGEN USE

DM yield and N uptake data are presented from primary growth and two successive regrowths of perennial ryegrass treated with combinations of three N treatments for the primary growth viz. 0, 50 and 100 kg N/ha (PN) and four N treatments applied for the second growths, viz. 0, 33, 66 and 100 kg N/ha (SN). Primary growth gave a response of 24·2 kg DM/kg applied N to PN50 with only a further l±0 kg DM/kg applied N from the second PN increment. A significant interaction between PN and SN treatments was shown in second growth. The residual DM response to PN was highest at SN0 and reduced as the level of SN was raised. Substantially greater residual responses to PN treatments were shown in the third growth. Third growth DM responses to SN treatments were high. High apparent recovery of fertilizer N reached 111% of primary N where SN66 followed PN50. High available soil N is partly responsible for both high apparent recovery of N and high DM response. The latter appears to be associated with inclusion of growths given no fertilizer N so that the full residual effects of fertilizer N and ‘priming’ of available soil N can be realised.     

THE EFFECT OF INCLUDING DRIED GRASS IN THE SUPPLEMENT GIVEN TO LACTATING COWS AT PASTURE

An investigation into the effect of including dried grass in the supplement given to dairy cows at pasture is reported. Supplements containing 0, 50 and 100% dried grass were used in the investigation which was carried out in two parts. In the first part 12 high-yielding cows with unrestricted access to pasture were used in an experiment with a change over design to assess the effects of feeding 5 kg/day of each supplement on milk yield and composition. In the second part 8 steers, housed in stalls and given cut herbage from the same sward as that grazed hy the cows, were used in a change over experiment to assess the effects of the three supplements on herhage and total DM intake. No significant differences in milk yield between supplements were ohtained, the mean yields being 23·9, 24·5 and 23·9 kg/day for the supplements containing 0, 50 and 100% dried grass, respectively. Milk composition was also not significantly affected by including dried grass in the supplement. In the indoor feeding trial using the steers, the addition of 3·5 kg DM from the supplement resulted in an increase in total DM intake of only 1·3 kg/day. There was no significant difference hetween supplements on herbage and total DM intake.     

Enzyme activity in soils at subzero temperatures

Urease activity, phosphatase activity, and sulfatase activity were detected in soils at ?10 and ?20°C. The occurrence of enzyme activity in soils at subzero temperatures is attributed to enzyme-substrate interaction in unfrozen water at the surfaces of soil particles. Support for this explanation was obtained from experiments showing that hydrolysis of urea by jackbean urease occurs at ?10 or ?20°C in the presence, but not in the absence, of clay minerals or autoclaved soils. No enzyme activity could be detected in soils at ?30°C.     

SWARD PRODUCTIVITY UNDER ALTERNATE OR REPEATED CUTTING OF ADJACENT SMALL AREAS

Cutting patterns producing high and low areas were imposed on swards of Dactylis glomerata and Lolium perenne to test whether output could be increased when compared with traditional uniform cutting methods. In two experiments, swards of both grasses were divided into parallel 9 cm strips which were cut alternately on different occasions to a height of 5 cm. These swards yielded a similar quantity of herbage DM to that from other swards cut uniformly at the same height and frequency. In a third experiment, swards of Lolium perenne were divided into alternate strips of unequal width (9 and 4.5 cm). The narrow strips were allowed to grow to a height of 15 cm and were occasionally trimmed at this height. The wide strips were cut to 5 cm at 2–or 4–weekly intervals. Areas cut to 5 cm every 2 weeks yielded significantly more DM when adjacent to high areas than when adjacent to areas cut to 5 cm every 2 weeks (i.e. under uniform cutting). The interaction between adjacent areas having different defoliation regimes is discussed in relation to the possibility of obtaining some additional output from a suitable no niform cutting system compared with uniform cutting. Sward productivity under no niform grazing patterns of livestock is also discussed.