Variation in prey quantity and quality can influence growth and survival of marine predators, including anadromous fish that migrate from freshwater systems. The objective of this study was to examine the energy dynamics of subyearling Chinook salmon (Oncorhynchus tshawytscha) following freshwater emigration. To address this objective, a population of Chinook salmon and their marine prey were repeatedly sampled from June to September over 2 years in coastal waters off Oregon and Washington. Subyearlings from the same population were also reared under laboratory conditions. Using a bioenergetics model evaluated in the laboratory, we found that growth rate variability in the field was associated more with differences in northern anchovy (Engraulis mordax) consumption and less with variation in diet energy density or ocean temperature. Highest growth rates (2.43–3.22% body weight/day) occurred in months when anchovy biomass peaked, and the timing of peak anchovy biomass varied by year. Our results support a general pattern among subyearling Chinook salmon occurring from Alaska to California that feeding rates contribute most to growth rate variability during early marine residence, although dominant prey types can differ seasonally, annually, or by ecosystem. In the northern California Current, faster growth appears to be associated with the availability of age‐0 anchovy. Identifying factors that influence the seasonal development of the prey field and regulate prey quantity and quality will improve understanding of salmon growth and survival during early marine residence. 相似文献
Field applications usingBacillus thuringiensis (Dipel 2 X) againstSpodoptera exigua infesting corn and sunflower cultivations were carried out in two different localities in Egypt. The percentage of infested corn plants decreased to 2.5%, seven days after treatment with Dipel 2x, (250 g/fed) and the yield was significantly increased compared with untreated plots. The carbamate insecticide (Lannate) at 300 g/fed caused complete kill to the larvae and the highest yield was obtained. Application of Lannate, Dipel (250 g) or a combination of both (150 g/each/fed) caused a high reduction of the larvae existing in sunflower after seven days from application, while Dipel at 200 g/fed gave a lower effect. Ten days after application, the number of larvae and infested plants increased significantly in plots treated with Lannate alone, while plots treated with either Dipel (250 g) or Dipel combined with Lannate (150 g/each) still maintained a lower infestation. The estimated yield showed the highest increase in plots treated with Lannate followed by Dipel (250 g) and then a combination of both. 相似文献
ABSTRACT: To utilize fisheries waste products as food materials with functional properties, shrimp head protein hydrolysates (SHPH) from three species of shrimp, that is, Northern pink shrimp ( Pandalus eous ), Endeavour shrimp ( Metapenaeus endeavouri ) and Black tiger shrimp ( Penaeus monodon ), were produced by enzymatic hydrolysis using endopeptidase derived from Bacillus subtilis and exopeptidase derived from Aspergillus oryzae at a level of 0.1% (w/w). SHPH were rich in protein (90–91%) and amino acids (71–84%) but little fat (0.01–0.02%). The average molecular weight of SHPH was 300–1400. The effect of 5% SHPH (dry basis) addition on the state of water and denaturation of lizard fish myofibrils (Mf) during the dehydration process was evaluated by the desorption isotherm and the Ca-ATPase activity, and compared with the effect of sodium glutamate (Na-Glu). SHPH decreased the water activity and the Ca-ATPase inactivation, and increased monolayer sorbed water and multilayer sorbed water of Mf, although these effects of SHPH were smaller than those of Na-Glu. These findings suggest that the SHPH suppressed dehydration-induced denaturation of myofibrillar protein by stabilizing the hydrated water surrounding myofibrils. 相似文献
The objectives of this study were to investigate the determinants of the anion gap (AG) in cattle and to evaluate the utility of AG in detecting hyperlactatemia in sick neonatal calves and adult cattle. The AG was calculated as AG = ([Na+] + [K+]) - ([Cl-] + [HCO3]), with all values in mEq/L. The AG of healthy neonatal calves (n = 16) was 29.6 ± 6.2 mEq/L (mean ± SD), and the blood L-lactate concentration ranged from 0.5 to 1.2 mM/L. The AG was significantly (P > .05) correlated with serum phosphate (r = .66) and creatinine (r = .51) concentrations. The AG of neonatal calves with experimentally induced diarrhea (n = 16) was 28.6 ± 5.6 mEq/L, and the blood L-lactate concentration ranged from 1.1 to 2.9 mM/L. The AG was significantly correlated with blood L-lactate concentration (r = .67), serum phosphate concentration (r = .63), creatinine concentration (r = .76), and blood pH (r = -.61). The AG of adult cattle with abomasal volvulus (n = 41) was 20.5 ± 7.8 mEq/L, and the blood L-lactate concentration ranged from 0.6 to 15.6 mM/L. The AG was significantly correlated with blood L-lactate concentration (r = .60), serum phosphate concentration (r = .71), creatinine concentration (r = .65), albumin concentration (r = .47), total protein concentration (r = .54), blood pyruvate concentration (r = .67), and blood pH (r = -.41) but not plasma β-OH butyrate concentration. The results indicate that the AG in cattle is only moderately correlated with blood L-lactate concentration and is similarly correlated with serum phosphate and creatinine concentrations in neonatal calves and adult cattle, as well as with serum albumin and total protein concentrations in adult cattle. Anion gap determination is of limited usefulness in predicting blood L-lactate concentration in sick cattle, whereas the correlation between AG and serum creatinine concentration in sick cattle suggests that an increased AG should alert the clinician to the potential presence of uremic anions. 相似文献
Increasing the lactose content of different milk replacers or milk diets by approx. 30 % of the dry matter increased the frequency of diarrhoea the first 10–12 days in young calves on all occasions in 5 experiments comprising 120 calves. For all diets taken together, this effect was highly significant. Total daily intakes of lactose amounted to 200–480 g. When lactose was given on top of the milk rations, the growth rate increased significantly, whereas the growth rate was usually insignificantly reduced when lactose replaced other nutrients in milk diets or milk replacers, their levels of protein and fat becoming low. Albumin and total protein in blood plasma were significantly lower when the dietary protein level was low. Milk replacers with 20 or 40 % whey powder, replacing skim milk powder, performed equally well, but gave significantly less growth than the old-fashioned feeding of whole milk-skim milk. Intake of hay and barley and a number of clinical and histological or pathological parameters did not vary consistently with dietary level of whey powder or lactose. Feeding whole milk all the time resulted in low intake of hay and barley and poorly developed forestomachs, but high dressing-out %. Substituting soya for part of the skim milk powder in milk replacers gave abomasal content with no curds. In most cases, pH in the rumen appeared to be nearly up to neutral until the calves ate ground barley, about 1 month old. 相似文献
An open radial fracture in an adult horse (450 kg) was repaired by internal fixation, using two 18-hole 4.5-mm broad dynamic compression plates and 5.5- and 4.5-mm bone screws. The fracture healed completely, but when evaluated 9 months after surgery, the horse was lame on the fractured limb at a trot. Local infiltration of anesthesia along the distal half of the bone plates greatly ameliorated the lameness, suggesting that the plates were irritating the soft tissues and extensor tendons along the cranial and lateral aspects of the antebrachium. Both bone plates were removed simultaneously with no complications, and the horse became sound. 相似文献
Field experiments were conducted to determine the effects of tillage (ploughing followed by one rotary hoeing (CT), rotary hoeing (MT) and no-tillage (NT)) and type of fertilization (NP 154/75 kg ha−1, digested farmyard manure 30 t ha−1) on growth (biomass and roots), N-accumulation and nodulation of vetch plants (Vicia sativa cv. Alexander) at different stages. The highest dry matter yields and root weight were determined for the NT and the lowest for the CT systems. The NT system promoted nodulation of vetch plants to a higher degree than the MT and CT systems. At the time just before harvesting, the N-accumulation in the above ground plants, was 54.3 for CT, 76.7 for MT, and 109.0 kg ha−1 for NT, and the corresponding values at roots were 73.3, 112.0 and 173.3 kg ha−1. Growth and nodulation of vetch plants was in general much better on farmyard manure plots than it was with the NP fertilization. The closest correlation coefficients between dry matter of biomass and roots on the one hand, and number of nodules and percentage N in roots on the other, were found with NT. The highest correlation between nodulation and percentage N in roots (r = 0.91***) was observed at the 128-days-old vetch plants. The highest correlation between the number of nodules and percentage N in biomass (r = 0.82**) was found at the beginning of the plants' flowering period. *, **, *** indicate significance at P = 0.05, 0.01 and 0.001 respectively. 相似文献
This work aimed to study the effect of long-term polymetallic contamination on the state and parameters of soil bacterial communities, including the abundance of different groups of culturable bacteria and the activity of nitrification.
Materials and methods
Monitoring plots were located in the dry lake and surrounding area, which had been formerly used for the discharge of industrial waste. The soils in the 16 plots were characterized by extremely high levels of heavy metal pollution. This study evaluated the main soil physicochemical properties by various methods, total metal contents by X-ray analysis, mobile metal content by atomic absorption spectrophotometry, the abundance of chosen groups of culturable bacteria by inoculation on solid media, and nitrification activity from ammonium and nitrite oxidation rates.
Results and discussion
High adaptation capacity of microbial communities to long-term pollution was revealed through marked lack of decrease in the abundance of some of the bacterial groups in soils with high contamination levels. Among the bacteria determined by the colony count method, copiotrophic and spore-forming bacteria were the least sensitive to contamination, and actinomycetes were the most sensitive. The high levels of soil pollution with heavy metals had pronounced adverse effects on nitrification activity. The decrease in activity was strongly correlated with pollutant concentrations. The oxidation of nitrite was shown to be more affected by pollution that the oxidation of ammonium.
Conclusions
Some groups and parameters of culturable microorganisms can be used for soil status estimation under pollution conditions even though they are only a small fraction of the microbial community. The most sensitive parameter was the nitrification rate, while the number of actinomycetes was found to be most promising parameter among the groups of bacteria determined by plate counts. The use of sensitive groups of culturable microorganisms for bioindication purposes is a method, which may provide a cheap and sufficiently reliable tool for large-scale soil monitoring studies.
124 wheat cultivars and breeding lines were screened with 19 microsatellite (SSR) loci generating 160 scorable bands which
were used to construct a genetic distance (GD) matrix. A distance matrix based on coefficient of parentage (COP) scores was
also generated for the cultivars for which good pedigree records were available. The SSR and COP data for 101 of the wheat
cultivars were compared with genetic distance scores obtained using1898 scorable restriction fragment length polymorphism
(RFLP) bands previously generated. Phylograms were generated based on the SSR, RFLP,combined SSR and RFLP and COP data. The
standardised Mantel's Z test showed that the distance matrices generated from all of the data sets were significantly correlated.
Bootstrap analysis showed that, although the SSR and RFLP data were correlated, a large number of SSR loci are required for
determining robust genetic relationships between large numbers of cultivars. In addition, accurate pedigree records are needed
to determine genetic relatedness using COP. The molecular data were also used to determine the level of genetic variability
within breeding programs and to assess the impact of the introduction of semidwarf and other germplasm. The results showed
that the level of genetic diversity in Australian wheat cultivars has increased over time and that in particular, the introduction
of semidwarf germplasm resulted in an increase in the overall diversity.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献