全文获取类型
收费全文 | 266篇 |
免费 | 8篇 |
专业分类
林业 | 15篇 |
农学 | 19篇 |
基础科学 | 8篇 |
74篇 | |
综合类 | 32篇 |
农作物 | 16篇 |
水产渔业 | 2篇 |
畜牧兽医 | 81篇 |
园艺 | 7篇 |
植物保护 | 20篇 |
出版年
2023年 | 3篇 |
2022年 | 4篇 |
2021年 | 1篇 |
2020年 | 4篇 |
2019年 | 12篇 |
2018年 | 5篇 |
2017年 | 2篇 |
2016年 | 10篇 |
2015年 | 3篇 |
2014年 | 2篇 |
2013年 | 14篇 |
2012年 | 16篇 |
2011年 | 21篇 |
2010年 | 9篇 |
2009年 | 7篇 |
2008年 | 19篇 |
2007年 | 10篇 |
2006年 | 15篇 |
2005年 | 17篇 |
2004年 | 9篇 |
2003年 | 9篇 |
2002年 | 10篇 |
2001年 | 2篇 |
2000年 | 10篇 |
1999年 | 4篇 |
1998年 | 4篇 |
1997年 | 4篇 |
1996年 | 2篇 |
1995年 | 5篇 |
1994年 | 2篇 |
1993年 | 6篇 |
1992年 | 6篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1981年 | 3篇 |
1980年 | 1篇 |
1977年 | 1篇 |
1975年 | 1篇 |
1973年 | 2篇 |
1972年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有274条查询结果,搜索用时 359 毫秒
251.
The development of a simulation model, "JohneSSim", was part of a research program aimed at designing a national Johne's disease control program for The Netherlands. Initially, the focus was mainly directed towards different compulsory "test-and-cull" strategies. However, the results from the JohneSSim model showed that eradication of Johne's disease based on such strategies would not be possible within 20 years and that it was also economically unattractive. However, improved calf management seemed to be more effective in reducing the prevalence within the same time period. Simulation of a strategy using an "ideal test" (80% sensitivity in all infected animals) showed a considerably faster decrease in prevalence. However, this strategy proved to be economically unattractive because of the high culling rate of (young) test-positive animals. The simulation model was also adapted to study beef cow herds. However, the results indicated that none of the strategies were able to reduce the mean true prevalence to almost zero for such herds. Only strategies based on "separation of calves and adult animals" proved to significantly reduce the prevalence but such a strategy is unpractical and uneconomic for Dutch beef cow herds. Due to this finding and the relative low number of Dutch beef cow farms, first priority has been given to the development of a Johne's disease control program for dairy farms. Based on the results of the "JohneSSim" model, the new national voluntary Johne's disease control program for dairy, Paratuberculosis Program Netherlands (PPN), started in September 2000. The PPN is based on a stepwise improvement of calf hygiene, with little dependency on "test and culling". The model results indicated that, if dairy farmers consistently carried out the necessary management adaptations, PPN considerably decreased prevalence and that it was economically more attractive than any previous plans. 相似文献
252.
Muscle biopsies from six horses with clinical histories of muscle atrophy, muscle tremors, myopathic symptoms, unsteadiness of pelvic limbs and progressive ataxia were examined. Muscle biopsies were studied with enzyme histochemical techniques to evaluate the diagnostic values of these methods in cases suspected of suffering from neuromuscular disorders. Hypertrophy, atrophy, fibre splitting, waxy degeneration, phagocytosis and necrosis were seen in haematoxylin eosin stained sections of the different cases. Fibre type predominance and fibre type grouping were seen in the calcium ion stimulated myosine ATP-ase (Ca-ATP-ase) stained sections of some cases. 'Moth-eaten fibres' were demonstrated in three cases by staining with NADH: nitro blue tetrazolium oxidoreductase (NADH-TR), succinate dehydrogenase (SDH), NADH dependent malate dehydrogenase, cytochrome c oxidase and by lactate dehydrogenase. The catabolic enzymes, acid phosphatase (ACP) and 5'-nucleotidase were active in cases with fibre phagocytosis. The oxidative part of the pentose phosphate pathway in myopathic tissue seemed to be important in three cases, demonstrated by the increased activity of glucose-6-phosphate dehydrogenase (GPDH) and 6-phosphogluconate dehydrogenase (PGDH). The important feature of diseased horse muscle was that the pathohistochemical changes were exactly the same as in diseased skeletal muscles of humans. The application of tissue saving enzyme histochemical techniques can be recommended in the study of muscle tissue from horses suffering from suspected neuromuscular disorders. 相似文献
253.
In this study, we reviewed the research that has been conducted in relationship with the problem of 'chronic wasting' in dairy cows in the Netherlands as was experienced during 1998 and 1999. Emphasis was drawn towards three aspects of this 'chronic wasting'; the definition, the magnitude, and the possible cause or causes. It appeared that a clear, objective definition of 'chronic wasting' in cows, and of farms with a problem of 'chronic wasting' cows is still lacking. Furthermore, the incidence (of the problem) was restricted to approximately 150 dairy farms at most. Currently, no farms with 'chronic wasting' cows are known. Combined with the uncertainty in the definition, this will hamper future research. Many of the studies were related to the hypothesis that the vaccination with the BHV1 marker vaccine had caused 'chronic wasting' in the cows. None of the results, however, substantiated this hypothesis. Other possible causes have hardly been investigated and further research would be needed to evaluate the role of nutrition, infectious agents, and genetics. However, due to the waning of the phenomenon, the availability of data and controls will be limiting. 相似文献
254.
Engel B Buist W Orsel K Dekker A de Clercq K Grazioli S van Roermund H 《Preventive veterinary medicine》2008,86(1-2):124-138
The sensitivity and specificity of six ELISA tests for foot-and-mouth disease (FMD) to discriminate between sero-converted (for non-structural FMD virus proteins) and non-sero-converted cattle were evaluated for vaccinated and unvaccinated cattle. Since none of the tests could be considered as a proper reference test and for about half of the tested sera the true status (sero-converted or not for non-structural proteins, i.e. presence of antibodies) of the animals was unknown, a Bayesian analysis employing a latent class model was used that did not rely on the use of a reference test or gold standard. Prior information about prevalence for subsets of the data and specificity of the tests was incorporated into the analysis. The specificity of the six tests for vaccinated and non-vaccinated cattle ranged from 96 to 99%. For vaccinated cattle, one test stood out with an estimated sensitivity of 94% (95% CI from 89.8 to 98.1%). Second best for vaccinated cattle were two tests with estimated sensitivities of 85% (95% CI from 78.9 to 89.7%) and 92% (95% CI from 86.2 to 95.6%). For non-vaccinated cattle, the sensitivities of these three tests were around 97%. The remaining three tests showed lower estimated sensitivity for vaccinated cattle, ranging from 57 to 79%. 相似文献
255.
Suwankong N Meij BP Van Klaveren NJ Van Wees AM Meijer E Van den Brom WE Hazewinkel HA 《Veterinary surgery : VS》2007,36(5):423-431
Objective— To evaluate the long-term outcome after decompressive surgery in dogs with degenerative lumbosacral stenosis (DLS), using force plate analysis (FPA) and owner questionnaires.
Study Design— Prospective clinical study.
Animals— Dogs with DLS (n=31) and 24 clinically normal Labrador Retrievers.
Methods— FPA was performed before surgery (31 dogs) and at 3 days, 6 weeks, 6 months (each, 31 dogs) and ≥1.5 years (12 dogs) after surgery. Peak vertical forces (Fz+ ), braking (Fy+ ), and propulsive forces (Fy− ), and the corresponding impulses were determined. Questionnaires were completed by the owners before and at 6 months and ≥1.5 years after surgery.
Results— Fy− of the pelvic limbs and the ratio pelvic/thoracic of Fy− (P/TFy− ) were significantly smaller in dogs with DLS than controls. Fy− and P/TFy− decreased significantly 3 days after surgery, and increased during 6 months follow-up, but with long-term follow-up the values remained the same and were smaller than controls. From questionnaires there was a significant improvement after surgery compared with function before surgery. Most owners were satisfied with outcome after surgery.
Conclusions— Propulsive forces of the pelvic limbs in dogs with DLS are impaired and are partially restored by decompressive surgery.
Clinical Relevance— Owner's questionnaires illustrate that decompressive surgery restores function as perceived by owners whereas FPA continues to show impaired propulsive forces for the pelvic limbs. 相似文献
Study Design— Prospective clinical study.
Animals— Dogs with DLS (n=31) and 24 clinically normal Labrador Retrievers.
Methods— FPA was performed before surgery (31 dogs) and at 3 days, 6 weeks, 6 months (each, 31 dogs) and ≥1.5 years (12 dogs) after surgery. Peak vertical forces (Fz
Results— Fy
Conclusions— Propulsive forces of the pelvic limbs in dogs with DLS are impaired and are partially restored by decompressive surgery.
Clinical Relevance— Owner's questionnaires illustrate that decompressive surgery restores function as perceived by owners whereas FPA continues to show impaired propulsive forces for the pelvic limbs. 相似文献
256.
Marles-Wright J Grant T Delumeau O van Duinen G Firbank SJ Lewis PJ Murray JW Newman JA Quin MB Race PR Rohou A Tichelaar W van Heel M Lewis RJ 《Science (New York, N.Y.)》2008,322(5898):92-96
A commonly used strategy by microorganisms to survive multiple stresses involves a signal transduction cascade that increases the expression of stress-responsive genes. Stress signals can be integrated by a multiprotein signaling hub that responds to various signals to effect a single outcome. We obtained a medium-resolution cryo-electron microscopy reconstruction of the 1.8-megadalton "stressosome" from Bacillus subtilis. Fitting known crystal structures of components into this reconstruction gave a pseudoatomic structure, which had a virus capsid-like core with sensory extensions. We suggest that the different sensory extensions respond to different signals, whereas the conserved domains in the core integrate the varied signals. The architecture of the stressosome provides the potential for cooperativity, suggesting that the response could be tuned dependent on the magnitude of chemophysical insult. 相似文献
257.
258.
Mergers play an important role in galaxy evolution and are key to understanding the correlation between central-black hole mass and host-galaxy properties. We used the new technology of adaptive optics at the Keck II telescope to observe NGC 6240, a merger between two disk galaxies. Our high-resolution near-infrared images, combined with radio and x-ray positions, revealed the location and environment of two central supermassive black holes. Each is at the center of a rotating stellar disk, surrounded by a cloud of young star clusters. The brightest of these young clusters lie in the plane of each disk, but surprisingly are seen only on the disks' receding side. 相似文献
259.
Chiara Bertora Petra C.J. van Vliet Jan Willem van Groenigen 《Soil biology & biochemistry》2007,39(2):632-640
Earthworm activity has been reported to lead to increased production of the greenhouse gas nitrous oxide (N2O). This is due to emissions from worms themselves, their casts and drilosphere, as well as to general changes in soil structure. However, it remains to be determined how important this effect is on N2O fluxes from agricultural systems under realistic conditions in terms of earthworm density, soil moisture, tillage activity and residue loads. We quantified the effect of earthworm presence on N2O emissions from a pasture after simulated ploughing of the sod (‘grassland renovation’) for different soil moisture contents during a 62-day mesocosm study. Sod (with associated soil) and topsoil were separately collected from a loamy Typic Fluvaquent. Treatments included low (L), medium (M) and high (H) moisture content, in combination with: only soil (S); soil+incorporated sod (SG); soil+incorporated sod+the anecic earthworm Aporrectodea longa (SGE). Nitrous oxide and carbon dioxide (CO2) fluxes were measured for 62 d. At the end of the incubation period, we determined N2O production under water-saturated conditions, potential denitrification and potential mineralization of the soil after removing the earthworms. Cumulative N2O and CO2 fluxes over 62 d from incorporated sod were highest for treatment HSGE (973 μg N2O-N and 302 mg CO2-C kg−1 soil) and lowest for LSG (64 μg N2O-N and 188 mg CO2-C kg−1 soil). Both cumulative fluxes were significantly different for soil moisture (p<0.001), but not for earthworm presence. However, we observed highly significant earthworm effects on N2O fluxes that reversed over time for the H treatments. During the first phase (day 3-day 12), earthworm presence increased N2O emissions with approximately 30%. After a transitional phase, earthworm presence resulted in consistently lower (approximately 50%) emissions from day 44 onwards. Emissions from earthworms themselves were negligible compared to overall soil fluxes. After 62 d, original soil moisture significantly affected potential denitrification, with highest fluxes from the L treatments, and no significant earthworm effect. We conclude that after grassland ploughing, anecic earthworm presence may ultimately lead to lower N2O emissions after an initial phase of elevated emissions. However, the earthworm effect was both determined and exceeded by soil moisture conditions. The observed effects of earthworm activity on N2O emissions were due to the effect of earthworms on soil structure rather than to emissions from the worms themselves. 相似文献
260.
Jan Willem Erisman 《Water, air, and soil pollution》1993,71(1-2):51-80
In this paper estimates of dry and wet deposition of acidifying substances in the Netherlands are presented. The deposition was estimated from measured concentrations in the atmosphere and in precipitation or if these were not available, from modelled concentrations. The method was applied for the Netherlands on a 5×5 km scale. The most important components are sulphur oxides and ammonia and their reaction products. It was estimated that the annual average deposition of SO x in the Netherlands decreased from 1570 to 670 mol ha?1 a?1 between 1980 and 1989. In 1989, the annual average NO y deposition was estimated to be 1220 mol ha?1 a?1 in 1980 and 1160 mol ha?1 a?1 in 1989. The annual average NH x deposition in the Netherlands was estimated to be 2330 and 2190 mol ha?1 a?1 in 1980 and 1989 respectively. HCI deposition was about 100 mol ha?1 a?1 in all years. Dry deposition contributes most to the total deposition for each component. The spatial distribution of the total deposition shows a gradient over the Netherlands with highest values in the South and lowest in the North of the country. Meteorological conditions are also of influence on the deposition fluxes for all components. During 1988 and 1989 meteorological conditions favoured low deposition. The estimated uncertainty in the average fluxes of SO x , NO y , and NH x for the Netherlands is 15, 25, and 30% respectively. The wet deposition fluxes can be estimated more accurately than the dry deposition. 相似文献