Maximizing the relationship of yield to site-specific management zones with object-oriented segmentation of hyperspectral images

Quick and low cost delineation of site-specific management zones (SSMZ) would improve applications of precision agriculture. In this study, a new method for delineating SSMZ using object-oriented segmentation of airborne imagery was demonstrated. Three remote sensing domains—spectral, spatial, and temporal- are exploited to improve the SSMZ relationship to yield. Common vegetation indices (VI), and first and second derivatives (\(\rho^{\prime}\), \(\rho^{\prime\prime}\)) from twelve airborne hyperspectral images of a cotton field for one season \(\rho^{\prime}\) were used as input layers for object-oriented segmentation. The optimal combination of VI, SSMZ size and crop phenological stage were used as input variables for SSMZ delineation, determined by maximizing the correlation to segmented yield monitor maps. Combining narrow band vegetation indices and object-oriented segmentation provided higher correlation between VI and yield at SSMZ scale than that at pixel scale by reducing multi-resource data noise. VI performance varied during the cotton growing season, providing better SSMZ delineation at the beginning and middle of the season (days after planting (DAP) 66–143).The optimal scale determined for SSMZ delineation was approximately 240 polygons for the study field, but the method also provided flexibility enabling the setting of practical scales for a given field. For a defined scale, the optimal single phenological stage for the study field was near July 11 (DAP 87) early in the growing season. SSMZs determined from multispectral VIs at a single stage were also satisfactory; compared to hyperspectral indices, temporal resolution of multi-spectral data seems more important for SSMZ delineation.     

Why must we rush to bury our dead (pigs): The option of excarnation by exposure

The accepted paradigm of foreign animal disease preparedness in Canada, the emergency for which to prepare, starts with identification of the exotic viral agent in a Canadian farm animal population. This narrative focuses on the containment of the infectious agent, within diseased animals, on infected premises. Framing the emergency as a disease incursion limits rational imagination to only one version of one potential animal emergency. This framing of the problem directs the carcass disposal solutions to consider only methods to dispose of viral infected material. However, in all documented responses to catastrophic swine diseases in the past three decades, the number of uninfected animals caught up in movement control zones and killed greatly exceeds the number of infected animals killed. The temporary closures of slaughterhouses in spring 2020 due to COVID-19 transmission resulted in thousands of healthy market hogs surplus to market; an unanticipated emergency of healthy pigs. This paper proposes an alternate carcass disposal option for material from uninfected farms. “Excarnation by exposure” is a natural process of debulking and dehydrating carcasses by blow fly larvae, mitigating financial costs of final disposal. Excarnation by exposure is a reasonable and possibly necessary additional option for the management of uninfected carcasses in a catastrophic emergency response in commercial pigs.     

The structure and regulation of the Irish equine industries: Links to considerations of equine welfare

The equine industries in Ireland are vibrant and growing. They are broadly classified into two sectors: Thoroughbred racing, and sports and leisure. This paper describes these sectors in terms of governance, education and training in equine welfare, and available data concerning horse numbers, identification, traceability and disposal. Animal welfare, and specifically equine welfare, has received increasing attention internationally. There is general acceptance of concepts such as animal needs and persons' responsibilities toward animals in their care, as expressed in the 'Five Freedoms'. As yet, little has been published on standards of equine welfare pertaining to Ireland, or on measures to address welfare issues here. This paper highlights the central role of horse identification and legal registration of ownership to safeguard the health and welfare of horses.     

A comparison of gross pathology,histopathology, and mycobacterial culture for the diagnosis of tuberculosis in elk (Cervus elaphus).

Using the isolation of Mycobacterium bovis as the reference standard, this study evaluated the sensitivity, specificity and kappa statistic of gross pathology (abattoir postmortem inspection), histopathology, and parallel or series combinations of the two for the diagnosis of tuberculosis in 430 elk and red deer. Two histopathology interpretations were evaluated: histopathology I, where the presence of lesions compatible with tuberculosis was considered positive, and histopathology II, where lesions compatible with tuberculosis or a select group of additional possible diagnoses were considered positive. In the 73 animals from which M. bovis was isolated, gross lesions of tuberculosis were most often in the lung (48), the retropharyngeal lymph nodes (36), the mesenteric lymph node (35), and the mediastinal lymph nodes (16). Other mycobacterial isolates included: 11 M. paratuberculosis, 11 M. avium, and 28 rapidly growing species or M. terrae complex. The sensitivity estimates of gross pathology and histopathology I were 93% (95% confidence limits [CL] 84.97%) and 88% [CL 77.94%], respectively, and the specificity of both was 89% [CL 85.92%]). The sensitivity and specificity of histopathology II were 89% (CL 79.95%) and 77% (CL 72.81%), respectively. The highest sensitivity estimates (93-95% [CL 84.98%]) were obtained by interpreting gross pathology and histopathology in parallel (where an animal had to be positive on at least one of the two, to be classified as combination positive). The highest specificity estimates (94-95% [CL 91-97%] were generated when the two tests were interpreted in series (an animal had to be positive on both tests to be classified as combination positive). The presence of gross or microscopic lesions showed moderate to good agreement with the isolation of M. bovis (Kappa = 65-69%). The results showed that post-mortem inspection, histopathology and culture do not necessarily recognize the same infected animals and that the spectra of animals identified by the tests overlaps.     

Concentrations of Prolactin, LH, Testosterone, TSH and Thyroxine in Normospermic Dogs of Different Breeds

Concentrations of prolactin (PRL), LH, testosterone (T), TSH and thyroxine (T₄) were determined before and at 20, 120 and 180 min after a single iv injection of thyrotropin-releasing hormone (TRH) in eight Beagles, eight Fox Terriers, six Labrador Retrievers and five Great Danes that were normospermic. Mean basal PRL concentrations were lower in the Fox Terriers compared with the Great Danes (p < 0.05). Mean LH concentrations were higher in the Fox Terriers than in the Beagles, and T was lower in the Fox Terriers at some times but not others (p < 0.05). Thyroid Stimulating Hormone (TSH) concentrations did not differ among breeds, while mean basal T₄ values were lower in Fox Terriers compared with Labrador Retrievers and Great Danes (p < 0.05). Stimulation of T₄ secretion 120 and 180 min after iv TRH injection was most pronounced in the Beagles and less in the Fox Terriers (p < 0.05). The results of the present study indicate that potential breed differences in circulating concentrations of PRL, LH, T, TSH and T₄ in male dogs with apparently normal fertility can be encountered, but further studies are needed to determine whether the observed differences are typical features of these breeds, reflect subsets of dogs within breeds, or are in part because of possible uncontrolled parameters such as sample timing, ambient photoperiod, housing conditions or diet.     

Effects of Gonadotrophin Releasing Hormone Administration on the Pituitary-Ovarian Axis in Anoestrous vs Ovariectomized Bitches

The aim of this study was to determine the effects of gonadotrophin releasing hormone (GnRH) administration on the plasma concentrations of reproductive hormones in intact and ovariectomized (OVX) bitches. Therefore, blood samples were collected at multiple times before and after the administration of 10 microg/kg GnRH (Fertagyl)) for the determination of the plasma concentrations of luteinizing hormone (LH), oestradiol, progesterone and testosterone in six anoestrus and in six OVX bitches. The mean plasma LH concentrations before and 60 min after GnRH administration were significantly lower in the anoestrous bitches than in the OVX bitches. In both groups GnRH administration resulted in a significant increase in the plasma LH concentration. The highest plasma LH concentrations were found at 10 min after GnRH administration and these values did not differ significantly between the two groups. Only in the anoestrous bitches a significant increase in plasma oestradiol concentrations was found after GnRH administration and these values were significantly higher than those in the OVX bitches. The plasma concentrations of progesterone and testosterone were low (close to or below the limit of quantitation) both before and after GnRH administration and the differences between anoestrous and OVX bitches were not significant. It can be concluded that (i) basal plasma LH concentration is significantly higher in OVX bitches than in anoestrous bitches, (ii) plasma LH concentration increases after GnRH administration in both anoestrous and OVX bitches, (iii) GnRH administration causes a significant rise in plasma oestradiol concentration only if ovarian tissue is present and (iv) measurement of plasma progesterone and testosterone concentrations before and after GnRH administration does not aid in distinguishing between anoestrous and OVX bitches. The results of this study may provide a basis for the diagnosis of remnant ovarian tissue and verification of neuter status in the bitch.