The sand seas of Titan: Cassini RADAR observations of longitudinal dunes

The most recent Cassini RADAR images of Titan show widespread regions (up to 1500 kilometers by 200 kilometers) of near-parallel radar-dark linear features that appear to be seas of longitudinal dunes similar to those seen in the Namib desert on Earth. The Ku-band (2.17-centimeter wavelength) images show approximately 100-meter ridges consistent with duneforms and reveal flow interactions with underlying hills. The distribution and orientation of the dunes support a model of fluctuating surface winds of approximately 0.5 meter per second resulting from the combination of an eastward flow with a variable tidal wind. The existence of dunes also requires geological processes that create sand-sized (100- to 300-micrometer) particulates and a lack of persistent equatorial surface liquids to act as sand traps.     

Loop‐mediated isothermal amplification (LAMP) assays for rapid detection of Pyrenopeziza brassicae (light leaf spot of brassicas)

Pyrenopeziza brassicae (anamorph Cylindrosporium concentricum) is an ascomycete fungus that causes light leaf spot (LLS) disease of brassicas. It has recently become the most important pathogen of winter oilseed rape (Brassica napus) crops in the UK. The pathogen is spread by both asexual splash‐dispersed conidia and sexual wind‐dispersed ascospores. Such inoculum can be detected with existing qualitative and quantitative PCR diagnostics, but these require time‐consuming laboratory‐based processing. This study describes two loop‐mediated isothermal amplification (LAMP) assays, targeting internal transcribed spacer (ITS) or β‐tubulin DNA sequences, for fast and specific detection of P. brassicae isolates from a broad geographical range (throughout Europe and Oceania) and multiple brassica host species (B. napus, B. oleracea and B. rapa). Neither assay detected closely related Oculimacula or Rhynchosporium isolates, or other commonly occurring oilseed rape fungal pathogens. Both LAMP assays could consistently detect DNA amounts equivalent to 100 P. brassicae conidia per sample within 30 minutes, although the β‐tubulin assay was more rapid. Reproducible standard curves were obtained using a P. brassicae DNA dilution series (100 ng–10 pg), enabling quantitative estimation of amounts of pathogen DNA in environmental samples. In planta application of the β‐tubulin sequence‐based LAMP assay to individual oilseed rape leaves collected from the field found no statistically significant difference in the amount of pathogen DNA present in parts of leaves either with or without visible LLS symptoms. The P. brassicae LAMP assays described here could have multiple applications, including detection of symptomless host infection and automated real‐time monitoring of pathogen inoculum.     

Inoculum potential of Sclerotinia sclerotiorum sclerotia depends on isolate and host plant

The soilborne fungus Sclerotinia sclerotiorum infects many important crop plants. Central to the success of this pathogen is the production of sclerotia, which enables survival in soil and constitutes the primary inoculum. This study aimed to determine how crop plant type and S. sclerotiorum isolate impact sclerotial production and germination and hence inoculum potential. Three S. sclerotiorum isolates (L6, L17, L44) were used to inoculate plants of bean, carrot, lettuce, oilseed rape (OSR) and potato, and the number and weight of sclerotia per plant quantified. Carpogenic germination of sclerotia collected from different hosts was also assessed for L6. Production of sclerotia was dependent on both crop plant type and S. sclerotiorum isolate, with OSR and lettuce supporting the greatest number (42–122) and weight (1.6–3.0 g) of sclerotia per plant. The largest sclerotia were produced on OSR (33–66 mg). The three S. sclerotiorum isolates exhibited a consistent pattern of sclerotial production irrespective of crop type; L6 produced large numbers of small sclerotia while L44 produced smaller numbers of large sclerotia, with L17 intermediate between the two. Germination rate and percentage was greatest for larger sclerotia (4.0–6.7 mm) and also varied between host plants. Combining sclerotial production data and typical field crop densities suggested that infected carrot and OSR could produce the greatest number (3944 m^?2) and weight (73 g m^?2) of S. sclerotiorum sclerotia, respectively, suggesting these crops potentially contribute a greater increase in inoculum. This information, once further validated in field trials, could be used to inform future crop rotation decisions.     

Inhibition of forage seed germination by leaf litter extracts of overstory hardwoods used in silvopastoral systems

Silvopastoral management strategies seek to expand spatial and temporal boundaries of forage production and promote ecosystem integrity through a combination of tree thinning and understory pastures. We determined the effects of water extracts of leaf litter from three species of overstory hardwood trees, yellow poplar, red maple, and white oak, on germination of common forage species: alfalfa, red and white clover, crabgrass, orchardgrass, and tall fescue without and with endophytes to increase understanding about potential interactions between silvopastures and overstory deciduous trees. Litter extracts reduced germination in red and white clover in a concentration dependent manner with greatest effects observed for poplar > maple > oak extracts. These reductions were linearly related to increasing osmolality and electrical conductivity of the leaf extracts. Modified Gompertz growth curve models, fit to data, further indicated treatment with litter extracts, especially poplar, delayed and slowed germination. Similarly, cumulative germination of a variety of grasses was inhibited by filtered 6 % (w/v) litter extracts with the effects of poplar > maple > oak. However, germination of all forages resumed after rinsing extract-treated seeds with water indicating inhibition could be due, in part, to osmotic effects of extracts or water-soluble allelopathic compounds. Final germination varied with forage species and extract type, but in most instances, did not reach the level of the control further suggesting specific ion toxicities as well. Management practices that account for the interactions between trees and forages are needed to ensure successful establishment and persistence of mixed species swards in silvopastoral systems.     

Don't go breaking apart: Anthropogenic disturbances predict meadow fragmentation of an endangered seagrass

1. Although it is well established that human activities are linked to the loss of seagrasses worldwide, the influence of anthropogenic disturbances on the habitat fragmentation of seagrass meadows is less understood. This information is essential to identify how humans are modifying seascapes and what disturbances pose the greatest risk to seagrasses, which is pertinent given the rapid urbanization occurring in coastal areas.
2. This study examined how the habitat fragmentation of an endangered seagrass Posidonia australis varied in relation to several anthropogenic disturbances (i.e. human population, marine infrastructure, terrestrial run-off and catchment land-usage) within 10 estuaries across 620 km of coastline in New South Wales, Australia.
3. When comparing between estuaries, the fragmentation of P. australis meadows was significantly greater in estuaries adjacent to highly populated metropolitan centres – generally in the Greater Sydney region. At sites within estuaries, the density of boat moorings was the most important predictor of habitat fragmentation, but there was also evidence of higher fragmentation with increased numbers of jetties and oyster aquaculture leases.
4. These results suggest that the fragmentation of seagrass meadows will become more pervasive as the human population continues to grow and estuarine development increases. Strategies to mitigate anthropogenic disturbances on seagrass meadow fragmentation could include prohibiting the construction of boat moorings and other artificial structures in areas where seagrasses are present or promoting environmentally friendly designs for marine infrastructure. This knowledge will support ongoing management actions attempting to balance coastal development and the conservation of seagrasses.

     

Land restoration management after topsoil mining and implications for restoration policy guidelines in New Zealand

A three-year field trial on an upland loessial soil (Belmont silt loam) in New Zealand investigated the effects of ripping, application of fertilizer N and grazing management on the recovery of some physical, chemical and biochemical properties of soil and pasture productivity following removal (mining) of topsoil. Removing the top 31 cm of soil by mining (all of the A horizon and part of the AB horizon) also removed most of the soil's labile organic matter fractions, and to a lesser extent its total organic matter. After three years, the microbial C and mineralizable N in the 0–10 cm depth of mined soil had reached 65 and 62 per cent of the corresponding levels in unmined soil. Ripping to a depth of 30 cm, application of fertilizer N and lenient grazing of the pasture failed to enhance the recovery of soil fertility. A soil sampling depth of 20 cm provided a reasonable basis to assess the microbial biomass and potential fertility. Pasture productivity was, on average, 30 per cent lower on mined land than on unmined land over the first three years after mining. Application of N proved uneconomic in terms of farm production, although a good pasture response to N fertilizer was obtained. The results from this and related trials are summarized as a series of recommendations for the monitoring and regulation of topsoil mining. An incentive regime is also recommended to encourage land owners and/or topsoil miners to use successful restoration techniques on topsoil-mined land. Copyright © 1999 John Wiley & Sons, Ltd.