rep-PCR法对稻瘟病菌有性后代随机群体的评价

构建稻瘟病菌有性后代随机群体是研究目的基因遗传特点，乃至克隆目的基因的重要基础，但是在建立有性后代随机群体时，可能会有无性后代污染。采用先分离单个子囊，待其长成菌落并产生分生孢子后，再分离单个萌发的分生孢子的方法构建稻瘟病菌有性后代随机群体。利用基于重复DNA序列Pot2的rep-PCR方法对分离得到的有性后代进行DNA指纹分析，并评价构建的稻瘟病菌有性后代群体的质量。结果表明，构建的有性后代随机群体共包含176个后代。每个后代个体的DNA指纹图谱均与两亲本的DNA指纹图谱不一致；有性后代之间的DNA指纹图谱也彼此不一样，说明得到的有性后代个体都是由子囊孢子萌发形成的，且分离自不同的子囊。GUY11的rep-PCR扩增片段中的5个特异性片段的分离比例也都符合1:1的期望值，是按单个位点标记分离的，与已知的结果相吻合，说明有性后代随机群体的质量较高，不存在偏离现象，是一个较为理想的群体。同时，研究结果还得出基于Pot2的rep-PCR方法可以用来快速评价稻瘟病菌有性后代群体的质量。     

A Comparative Study of Confined Concrete Models under Cyclic Loading

In this paper,four models are selected as the representatives and applied to nonlinear dynamic analysis program for space frames which is based on the beam-column elements using the finite element flexibility method and the fiber model.Park's test on combined axial and bending columns under cyclic loading are taken as the calibrations,comparisons to material and structural member level are drawn and the analytical results of various models are discussed.     

福建省甘薯种质资源鉴定与评价

征集甄别保存甘薯种质资源606份,编写出品种目录,拍摄了彩色图谱册;摸索出适合福建省气候特点的田间种植、薯块贮藏、老蔓越冬和茎尖脱毒试管苗4种方法相结合的安全保存方法;首次在相同条件下同时完成了对保存的品种资源抗蔓割病、薯瘟病的鉴定,8种主要营养成份的分析及鲜薯与薯干产量的测定,筛选出抗蔓割病资源212份、抗薯瘟病资源109份、高干率资源14份、高淀粉资源12份、高水溶性糖资源13份、高粗蛋白资源13份、高粗脂肪资源12份、低粗纤维资源12份、高维生素C资源12份、高胡萝卜素资源12份,初步摸清了这些品种的分布情况;应用Borlanddel-phi7.0为设计集成环境和通用SQL方式查询程序模块创建了资源数据库;建立了107份骨干资源圃。     

茶园套种牧草对作物产量及土壤基本肥力的影响

我国关于茶园生草和低层套(间)种研究起步很晚且报道甚少,有必要深入研究适宜我国茶园推广应用的低层茶草套(间)种模式。通过3年的田间小区试验,以不套种任何牧草处理为对照,研究了3种不同牧草[百喜草(Paspalum natatu)、白三叶(Trifolium repens)、平托花生(Arachis pintoi)]套种模式对茶青和鲜草产量及其茶园土壤基本肥力的影响。结果表明:3种不同牧草套种模式比对照提高茶青产量6.48%~20.26%,并收获鲜草产量1 352~4 023 kg·hm?2·a?1;3年来逐年减少茶园表土流失厚度幅度分别为25.0%~30.6%、33.3%~38.9%和53.3%~60.0%,并提高茶园土壤pH 9.64%~11.76%、有机质8.23%~76.13%、全氮13.59%~245.63%、全磷3.41%~210.51%、全钾1.37%~9.65%、碱解氮69.63%~109.33%和速效磷11.40%~34.20%;其中,茶园套种白三叶和套种平托花生处理对提高茶叶产量和茶园土壤基本肥力的效果相对更佳,值得在生产上进一步推广应用。     

酿酒曲对柑橘梨汁复合酒发酵工艺的影响

通过添加不同含量的酿酒曲到柑橘梨汁复合液进行试验,结果表明：酿酒曲具有提高果汁出汁率,从而提高酒精度的作用;适当提高酿酒曲含量有利于果汁含糖量的快速降解,可获得较高浓度的酒精     

氮肥施用对柑橘果园土壤有机碳矿化的影响

土壤有机碳矿化是土壤向大气释放CO2的最大净输出途径,其与植被的净初级生产力的差值是判断生态系统碳“源”或“汇”的关键。采用室内模拟试验,在10、20、30℃ 3个温度条件下,研究施肥施用对柑橘果园土壤有机碳矿化的影响。结果表明：3种温度处理下,各施氮处理土壤有机碳矿化速率都表现为培养前期快速下降,培养后期保持相对稳定的趋势。在整个培养过程中,3种温度条件下各施氮处理的土壤CO2累积排放量为1328.25~2219.42 mg/kg,100 mg/kg（N4）处理土壤有机碳矿化量最大,CK处理最低,100 mg/kg（N4）和80 mg/kg（N3）2个高氮处理显著高于低氮50 mg/kg（N2）、30 mg/kg（N1）处理。土壤有机碳矿化速率随温度升高而增长,不同的土壤施氮条件下土壤有机碳矿化的温度敏感性不同,N2处理土壤有机碳矿化的温度敏感性最低,N4处理最高。柑橘果园土壤有机碳矿化受高施氮量影响较大,低施氮影响不明显。随着施氮量的增加土壤有机碳矿化的温度敏感性增加,氮肥施用和温度的共同作用可能使柑橘林向大气中排放的CO2增加。     

Heterosis and combining ability of seven maize germplasm populations

Understanding the combining ability and heterosis of available germplasm is a prerequisite for successful maize improvement and breeding. The objectives of this study were to analyze the combining ability and heterosis of seven representative maize germplasm populations, and further, to evaluate their potential utility in germplasm improvement. A total of 21 crosses were made among these seven populations in a complete diallel without reciprocals. The parental populations and 21 crosses were evaluated for days to silking (DS), ear height (EH), and grain yield (GY) in the Northeast and Yellow and Huai River maize growing areas in China in 2012. Csyn5, Csyn7, Cpop.11, and Cpop.12 had desirable general combining ability (GCA) effects for DS and EH in both the Northeast China mega-environment (NCM) and the Yellow and Huai River Regions of China mega-environment (YHCM). Cpop.11 possessed a favorable GCA effect for GY in the NCM, as did Csyn5, Cpop.17, and Cpop.18 in the YHCM. Csyn6 and Csyn7 exhibited tremendous yield-enhancing potential in both mega-environments. Additionally, six combinations including Csyn7 × Csyn6, Csyn5 × Csyn6, Cpop.11 × Cpop.18, Cpop.12 × Cpop.17, Csyn7 × Cpop.17, and Csyn5 × Csyn7 exhibited better specific combining ability effects for GY, yield performance, and mid-parent heterosis in the appropriate mega-environment. These results indicated that the seven populations would be very useful for the improvement of related agronomic traits, and the six candidate combinations possessed great potential for further improvement and utilization.     

基于PDA的外来入侵物种数据采集系统设计与实现

为了提高外来入侵物种数据采集的规范性、准确性及数据传输的时效性,在分析外来入侵物种数据特点和数据采集需求的基础上,提出基于PDA的外来人侵物种数据采集系统的设计方案.系统以C#编程语言为开发工具,以SQL CE数据库为数据存储方式,结合GPS,WCF和Microsoft sync Framework等技术手段,实现了外来入侵物种数据采集、数据存储和数据上传等功能,提高了外来先入侵物种数据采集的工作效率.