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101.
102.
Weiser MG 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1985,14(2):7-9
Sulfate based cell counting diluent and human erythrocyte size standards were evaluated for electronically sizing erythrocytes of common domestic species. Mean differences between calculated and human cell referenced electronic mean corpuscular volume values were 0, 0.4, 0.8, and 2.1 fl for blood of dogs, horses, cats, and cows, respectively. These differences were statistically significant only for the cat (p=0.029) and cow (p=0.0002). Electronic mean corpuscular volume was measured on multiple lots of human cell control material following calibration with human cells and cells of each of the four species. There were no significant differences between assigned assay values and direct measurements at each calibration (F=0.14, df=29). Sulfate based diluent, used on some automated cell counting systems, appears suitable for sizing animal erythrocytes and commercially available human cell standards are appropriate for calibration of certain systems used in veterinary hematology. 相似文献
103.
The study demonstrates the common antigens to salivary gland, fat body, mesenteron, thorax muscle, native whole body, and dried whole body homogenates of tsetse flies, G. palpalis palpalis. The possibilities of their origin and the role in hypersensitivity induction and its propagation are discussed. 相似文献
104.
D Van Houten M G Weiser L Johnson F Garry 《American journal of veterinary research》1992,53(10):1773-1775
Hematologic values and cellular morphologic features were evaluated for 38 healthy adult llamas. Reference ranges were determined for PCV, reticulocyte concentration, leukocyte concentration, and leukocyte differential counts. The approach used in this study was to focus on hematologic values that may be determined by use of techniques readily available to the practicing veterinarian and nonveterinary laboratory. Unique cellular morphologic features commonly observed and interpreted as normal included large granular lymphocytes, hyposegmented eosinophil nuclei, folded erythrocytes, and hemoglobin crystals. 相似文献
105.
Alton C S Ward Glen C Weiser Bruce C Anderson Patrick J Cummings Karen F Arnold Lynette B Corbeil 《Canadian journal of veterinary research》2006,70(1):34-42
Respiratory disease and poor lamb recruitment have been identified as limiting factors for bighorn-sheep populations. Haemophilus somnus (recently reclassified as Histophilus somni) is associated with respiratory disease in American bison, domestic sheep, and cattle. It is also harbored in their reproductive tracts and has been associated with reproductive failure in domestic sheep and cattle. Therefore, reproductive tract and lung samples from bighorn sheep were evaluated for the presence of this organism. Organisms identified as H. somnus were isolated from 6 of 62 vaginal but none of 12 preputial swab samples. Antigen specific to H. somnus was detected by immunohistochemical study in 4 of 12 formalin-fixed lung tissue samples of bighorn sheep that died with evidence of pneumonia. Notably, H. somnus was found in alveolar debris in areas of inflammation. The 6 vaginal isolates and 2 H. somnus isolates previously cultured from pneumonic lungs of bighorn sheep were compared with 3 representative isolates from domestic sheep and 2 from cattle. The profiles of major outer membrane proteins and antigens for all of the isolates were predominantly similar, although differences that may be associated with the host-parasite relationship and virulence were detected. The DNA restriction fragment length profiles of the bighorn-sheep isolates had similarities not shared with the other isolates, suggesting distinct phylogenetic lines. All of the isolates had similar antimicrobial profiles, but the isolates from the bighorn sheep produced less pigment than those from the domestic livestock, and growth of the former was not enhanced by CO2. Wildlife biologists and diagnosticians should be aware of the potential of these organisms to cause disease in bighorn sheep and of growth characteristics that may hinder laboratory detection. 相似文献
106.
S.C. Schank R.L. Smith G.C. Weiser D.A. Zuberer J.H. Bouton K.H. Quesenberry M.E. Tyler J.R. Milam R.C. Littell 《Soil biology & biochemistry》1979,11(3):287-295
Bacteria associated with roots of grasses from Florida, Ecuador and Venezuela were isolated and their N2-fixing ability was demonstrated by C2H2 reduction assay. The bacterial isolates have been classified as Azospirillum brasilense (formerly Spirillum lipoferum). These N2-fixing isolates have been compared with several Brazilian strains. Fluorescent antibody (FA) techniques were used to assist identifying isolates of N2-fixing bacteria from grass roots. Tests with antisera prepared against four strains of Azospirillum were used to define serological groups. Antigen-antibody specificity was demonstrated using both Azotobacter and Azospirillum antisera against known species of other soil microorganisms and numerous unidentified soil bacteria. Several applications of the FA technique are suggested to identify N2-fixing bacteria associated with grass roots. 相似文献
107.
Mechanisms of quorum sensing and strategies for quorum sensing disruption in aquaculture pathogens 下载免费PDF全文
In many countries, infectious diseases are a considerable threat to aquaculture. The pathogenicity of micro‐organisms that infect aquaculture systems is closely related to the release of virulence factors and the formation of biofilms, both of which are regulated by quorum sensing (QS). Thus, QS disruption is a potential strategy for preventing disease in aquaculture systems. QS inhibitors (QSIs) not only inhibit the expression of virulence‐associated genes but also attenuate the virulence of aquaculture pathogens. In this review, we discuss QS systems in important aquaculture pathogens and focus on the relationship between QS mechanisms and bacterial virulence in aquaculture. We further elucidate QS disruption strategies for targeting aquaculture pathogens. Four main types of QSIs that target aquaculture pathogens are discussed based on their mechanisms of action. 相似文献
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Effect of Ca Ionophore On Blastocyst Production Following Intracytoplasmic Sperm Injection in Caprine Oocytes 下载免费PDF全文
SD Kharche J Pathak S Agarwal B Kushwah AKS Sikarwar 《Reproduction in domestic animals》2016,51(4):611-617
The aim of the present investigation was to study the effect of calcium ionophore activation on blastocyst production following intracytoplasmic sperm injection (ICSI) in in vitro‐matured Caprine oocytes. A total of 470 in vitro‐matured oocytes were selected and randomly divided in to three groups. Cumulus oocyte complexes (COCs) recovered by slicing the Caprine ovaries were matured in TCM199 supplemented with 10% foetal bovine serum (FBS) + 10% follicular fluid + FSH (5 μg/ml) + LH (10 μg/ml) + estradiol (1 μg/ml) + EGF (10 ng/ml) + BSA (3 mg/ml) for 27 h in humidified atmosphere at 38.5°C with 5% CO2 in CO2 incubator. After 27 h of culture, selected COCs (n = 470) were separated from cumulus cells by treating with 0.1% hyaluronidase enzyme and passing repeatedly through a fine pipette and randomly divided into three groups. In group 1, (n = 168) matured oocytes were injected with injection micropipette without sperm as control. In group 2, (n = 152) capacitated spermatozoa were injected into cytoplasm of in vitro‐matured oocytes through injection micropipette. In group 3, (n = 150) capacitated spermatozoa were injected into cytoplasm of in vitro‐matured oocytes through injection micropipette and then activated with 5 μm Ca ionophore for 5 min. The oocytes of all groups were then culture in RVCL media for embryo development. The cleavage rate was observed after 48–72 h of injection. The cleavage rate and blastocyst production in group 1, 2 and 3 were 0.00 and 0.00, 18.42 and 3.57 and 61.33% and 16.30%, respectively. The result indicated that mechanical activation failed to induce cleavage in in vitro‐matured Caprine oocytes, whereas chemical activation of intracytoplasmic sperm‐injected in vitro‐matured Caprine oocytes showed significantly higher cleavage rate and blastocyst production as compare to non‐activated oocytes. 相似文献