Screening, identification, and potential interaction of active compounds from Eucommia ulmodies leaves binding with bovine serum albumin

The aqueous extract of Eucommia ulmoides leaves has been commonly known as Du-zhong tea as a functional health food for the treatment of hypertension, hypercholesterolemia, and fatty liver. This study developed a centrifugal ultrafiltration-high-performance liquid chromatography (HPLC) method for screening and identification of bioactive compounds in E. ulmoides leaves binding with bovine serum albumin (BSA). Six active compounds were screened, isolated, and elucidated by their ultraviolet (UV), electrospray ionization-mass spectrometry (ESI-MS), and nuclear magnetic resonance (NMR) data as geniposidic acid (1), caffeic acid (2), chlorogenic acid (3), quercetin-3-O-sambubioside (4), rutin (5), and isoquercitrin (6). The interaction between active compounds and BSA was investigated in the absence and presence of other compounds by quenching the intrinsic BSA fluorescence. The results indicated that the structures significantly affected the binding process. The values of binding constants for compounds 2-6 were in the range of 10(5)-10(6) mol L(-1), while geniposidic acid (1) hardly quenching the BSA intrinsic fluorescence. However, the quenching process of geniposidic acid was easily affected in the presence of other active compounds. The formation of the geniposidic acid-phenylpropanoid (flavonoid) complex could increase the binding affinity of geniposidic acid with BSA; however, the increased steric hindrance of the complex may make phenylpropanoid or flavonoid dissociate from BSA and then decrease their affinities.     

石河子垦区猪场五种病原混合感染的血清学调查

为了解石河子垦区规模猪场猪圆环病毒2型(PCV-2)与猪肺炎支原体(Mhp)、胸膜肺炎放线杆菌(App)、副猪嗜血杆菌(Hps)、猪链球菌2型(SS2)混合感染情况,采集垦区16个猪场420份血清进行了PCV-2与Mhp、App、Hps、SS2混合感染的血清学调查.结果显示,猪场PCV-2感染率达100％ (16/16),PCV-2感染阳性率为80.95％(340/420),PCV-2与Mhp、App、Hps、SS2双重混合感染率分别达53.82％(183/340)、43.82％(149/340)、64.42％(219/340)、45.1％(158/340);三重混合感染率为15％～36.47％,四重混合感染为13.24％～25.88％,五重混合感染为10.59％.调查结果表明垦区规模猪场PCV-2感染及其与Mhp、App、Hps、SS2混合感染情况普遍存在.     

发展红麻产业 推动吉林生态省建设

红麻具有突出的生态效用，适应性广，生长迅速，是一种生物产量较高的经济作物。吉林省盐碱地面积达9677多公顷，恶劣的生态环境严重地影响当地的经济发展和人民生活，特别是西部地区生态建设是吉林生态省建设的重点，也是生态环境恢复和建设的难点。发展红麻产业，高效利用有限的土地资源，防止土壤退化、沙化和水土流失，减少造纸对森林资源的破坏，对维护吉林省生态平衡，促进生态省建设具有重要意义。     

河北省糜黍品种资源类型及分布特点研究

在较系统掌握河北省建国以来糜黍生产概况的基础上，通过对河北省６４４份糜黍品种资源材料的分析，提出并描述了河北省糜黍品种资源在生育期、株高、主穗长、主茎伸长节间数、穗型、花序色、粒色、米色、单株粒重、千粒重、落粒性等主要性状上的分类标准和分布特点，为河北省今后的糜黍生产及资源的深入研究利用奠定了基础。     

半封闭式南美白对虾精养技术

2006年,笔者结合宁波市渔业科技入户示范工程在宁海县大和水产养殖公司建立半封闭式南美白对虾精养示范基地,并通过示范基地的示范作用推广健康养殖技术。     

论建设云南生态农业的新模式——观光农业

 分析云南省农业资源状况，探讨建设生态农业及观光农业的发展方向，使农业与旅游业有机结合，良性循环，以促进农业的可持续发展。     

苹果种植GAP控制要点分析

苹果以其优良的品质特色畅销国际市场。在苹果生产基地执行良好农业规范(GAP),进一步提升苹果种植管理水平,是适应国际农产品市场准入规则,提升苹果产业效益的现实要求。在执行GAP过程中,苹果种植业应具有较好的资源优势和地缘优势,尤其表现在土壤、水、肥、植保等控制要点上有较好的执行基础。     

仔猪色氨酸研究新进展

<正>色氨酸(Trp)是仔猪日粮中排于赖氨酸、苏氨酸、蛋氨酸之后的第4限制性氨基酸。它除了有助于蛋白质沉积,最重要的作用就是对适口性和采食量的调     

Characteristics of Reproductive Biology for Larix Originating in Japan

SpeciesandDistributionofLarchThereare15specicsofLarixintheworld.Theymainlydistributeinthefrigid-te11lperatezo11coftI1eNortl1cr11Hclnispllcrcandtl1cH'-Jt,asinCI1ina.Tl1crearconI\'.onesPCCiesa11dOI1c.-'.rLtntrtvI1iclldistributci11Japan,l1all1cIy-Japa11cseIarcl1(Larirk'len1liferiCarr.)a11dDahurianlarcl1(LarirgIneli)1iivar../al)o11i`xIPiIgcr).JapaIlcscIarcl1distributcsi11al1arrotvarca.OI1l}'i11tIlcalpil1cinHOI1sllu.Tl1cnortl1ofl1orizol1taldistributionisi11MiyaglCouIll}'(alt.l56511…     

产奶量遗传潜力不同的荷斯坦犊牛在代谢刺激下的生理反应

按系统指数（ＰＩ：Ｐｅｄｉｇｒｅｅ Ｉｎｄｅｘ）的高低将１１９头北京荷斯坦犊牛分成两组（ＰＩ〉５００为高组，ＰＩ〈５００为低组），其中３８头犊牛（５头公牛的后代）具有高系谱指数，平均值为７１８．５，８１头犊牛（１０头公牛的后代）具有低系谱指数，平均值为４００．５。犊牛为３～６月龄，在禁食３４～３６ｈ状态下采血，检测它们血液中总蛋白（ＴＰ）、尿素氮（ＢＵＮ）、肌酸酐（ＣＲＥ）、游离脂肪酸（ＦＦＡ）的