Effect of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponense

This study examined the effects of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponese . The response of the cellular defense was determined by monitoring changes in reactive oxygen intermediates in hemocytes, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione reductase and glutathione transferase activity in muscle. The results showed that prawns fed the basal diet had a significant increase in reactive oxygen intermediates in response to exposure to ammonia-N. When dietary vitamin C was added to the feed, there was a significant decrease in reactive oxygen intermediates in response to exposure to ammonia-N. Total superoxide dismutase and glutathione peroxidase activity of prawns fed the basal diet decreased as ammonia-N increased. This decrease in activity was significantly less pronounced in prawns that were fed the dietary vitamin C supplement. In prawns that were fed the dietary vitamin C supplement there was no significant variation in catalase reductase or gluxathione reductase activity in response to variations in ammonia-N concentration. The glutathione transference activity increased at a low concentration of ammonia-N in prawns that were fed the dietary vitamin C supplement, but there was no significant variation in the prawns fed the basal diet. Overall the results indicate that dietary vitamin C supplements enhance the capacity of M. nipponese to respond to elevations in ammonia-N. This response involved several biochemical reactions known to be involved in cellular defense.     

污水污物潜水旋流泵性能及设计方法试验研究

对影响潜水旋流泵性能的6个主要过流件几何参数进行了试验研究，初步掌握了旋流泵叶轮及泵体主要几何尺寸对性能影响的规律。在分析16种优秀水力模型的基础上，计算出叶轮D2及b2函数回归方程式，并用设计实例进行检验，样机效率比国内外同类产品提高3％左右。     

Hematological parameters and visceral lesions relationships in rabbit viral hemorrhagic disease.

Twenty rabbits were inoculated with a suspension of Viral Hemorrhagic Disease virus. Hemostatic functions were assessed every sixth hour from 6 to 60 hours post-inoculation. Tissue samples obtained at the same intervals allowed the study of the development of lesions throughout the experiment. Biological signs of Disseminated Intravascular Coagulation (DIC) were detected on and after 30 h post-inoculation and consisted of prolonged One Stage Prothrombin Time and Activated Partial Thrombin Time, the decrease of factors V, VII, and X and high levels of soluble fibrin monomer complexes and D-dimers. A reduction of thrombocyte numbers, heterophils and lymphocytes was associated. The close association of DIC and necrotizing hepatitis lesions suggested the hepatic lesions to be the most important DIC triggering factor. Other mechanisms are discussed.     

Reproductive biology of the female Japanese mantis shrimp Oratosquilla oratoria (Stomatopoda) in relation to changes in the seasonal pattern of larval occurrence in Tokyo Bay, Japan

ABSTRACT:   The reproductive traits and the monthly larval abundance of the mantis shrimp Oratosquilla oratoria were investigated in Tokyo Bay, Japan, in 2002. The goal of the study was to elucidate the cause of changes in the monthly pattern of larval abundance from the 1980s to the 1990s as these changes relate to variation in the stock size of the adult shrimp. Oogenesis was divided into 10 stages by histological observation. The developmental stage of oocytes in an individual's ovary was synchronous, suggesting that almost all the oocytes in an ovary are spawned at the same time. The size at first maturity was estimated to be 7 ≤ body length ( BL ) < 8 cm. Fecundity was expressed as a function of BL , ranging from 19 300 eggs for 8 cm BL to 92 100 eggs for 14 cm BL . Small female shrimps (<10 cm BL ) spawned around August. Most large female shrimps (≥10 cm BL ) spawned around May, and some large female shrimps also spawned until September. Although most large female shrimps spawned in spring, the larval abundance was low before July and high from August onwards. The results suggest that a substantial decrease in the stock size of large individuals causes the low larval abundance before July.     

Modifying the response to defoliation during vegetative growth in CERES-Maize

CERES-Maize (Vl. 0) predicted no grain yield when 100% defoliation occurred during vegetative growth. This result conflicted with field observations where 100% defoliation early in the vegetative stage did not materially reduce grain yield. CERES-Maize was modified to realistically predict yields when defoliation greater than 50% occurred during vegetative growth by taking into account stem (stem proper and sheath) photosynthesis and translocation of dry matter from the stem to the developing leaves. With these modifications, predictions from CERES-Maize were in good agreement with field measurements when defoliation occurred both early and later in the vegetative stage. This modified version of CERES-Maize can be the basis of a decision support system for defoliating pests of corn where yields can be evaluated under different management strategies and climate scenarios.     

Characterization of and radioimmunoassay for canine thyroglobulin

Canine thyroglobulin (cTg) has been isolated and purified. It has similar electrophoretic patterns as Tg from other mammalian species. The main fraction had a MW of 660,000, whereas also fractions of a MW of approximately 1,300,000 (dimer) and 330,000 (subunit) were present. The iodine content was 0.8 to 1.0 % (w/w). cTg did not cross-react with antibodies against human Tg to a degree that would allow the use of a radioimmunoassay for human Tg for the determination of cTg in serum or plasma. Therefore a polyclonal antiserum was raised against cTg and a homologous radioimmunoassay was developed, which was sensitive (0.4 μg/l) and specific (cross-reactivity in cTg assay of human Tg, goat Tg, T₄, T₃, and DIT < 0.01 %).

Plasma Tg levels in normal dogs of both sexes and aged 3–15 years amounted to 192 ± 73 μg/l (mean ± SD, n=30). There was no relation between plasma Tg and T₄ levels.     

茄子早熟育种研究Ⅱ选择指数与早熟性选择

研究结果表明,由性状果实最大周长,果实体积,门茄显蕾期,单株产量等性状构成的选择指数较好;进一步进行遗传相关分析结果认为,应用选择指数时应注意果实体积这个性状,单株产量是一个重要的辅助性状,门茄显蕾期是一个限制性因子。应用选择指数对品种评价结果认为,早熟性较好的品种有:天津早茄、西安绿茄、二民、七叶茄、白线茄和汉中荷包。