Combined linkage maps and QTLs in sugar beet (Beta vulgaris L.) from different populations

The construction of genetic maps is an expensive and time-consuming process. The breeder is therefore interested in using maps developed from other mapping populations but this is only possible if the genetic structure is similar for the chromosomal regions of interest. In this paper, maps of three populations of sugar beet (Beta vulgaris L.) with common polymorphic marker loci are compared. Maps were constructed with MAPMAKER 3.0 and JOINMAP 2.0. Both mapping programs gave, in general, the same order for common markers. However, the number of common markers was too low to construct a combined map for all chromosomes. For one population, in contrast to the other two, the map constructed with MAPMAKER 3.0 was much longer than that constructed with JOINMAP 2.0. For two of these populations yield traits were also available from different environments. For quantitative trait loci (QTL) analysis of the yield data, the packages MAPMAKER/QTL 1.1 and PLABQTL were used. No QTL common for the two populations could be detected. The program and the version used strongly influenced the estimated positions of QTLs. There was also a strong interaction with environments.     

Use of LiDAR and supplemental data to estimate forest maturity in Charles County,MD, USA

This study evaluated the utility of remotely sensed data to estimate forest maturity within Charles County, MD. We calculated tree canopy height using airborne scanning LiDAR (light detection and ranging) data over the entire county, and compared this to crown top height, stand age, and other data collected from randomly selected plots on the ground. Canopy height was a strong predictor of forest age, and we improved predictive power by including other variables such as land cover, slope, stream proximity, wetlands, and floodplains. These comparisons allowed us to construct a spatial model classifying forest in the study area into three age categories: ≤30 years old, 30–70 years old, and >70 years old, corresponding to young, intermediate, and mature. This spatial model was used to help characterize ecosystem condition and wildlife habitat, and help prioritize conservation decisions in the study area.     

Effects of planting food crops on survival and early growth of timber trees in eastern Panama

Restoration of degraded pasture lands in the tropics through afforestation is widely supported. The greatest obstacle to afforestation, however, is the long delay before initial financial returns from wood harvesting are realized. Interplanting young trees with food or energy crops has been proposed as a strategy to help overcome this obstacle. We investigated the impact of this practice on the survival and growth performance of young tropical tree seedlings in Panama. Five native timber tree species and the exotic species Tectona grandis were interplanted with four different crop rotations and monitored over 2 years. Survival of young tree seedlings was up to eight times higher when planted in association with Manihot esculenta. Only during the first 3 months after maize sowing was a significant negative effect of intercropping on tree seedling survival found. Here, survival rate of tree seedlings was up to four times lower than in the pure plantation. Tree growth was not adversely affected by crops. In fact, Astronium graveolens, Cedrela odorata and Terminalia amazonia showed significantly superior growth performance in association with both Zea mays and Cajanus cajan. When combined with the latter, the height increment of these tree species was up to four times that achieved in pure plantations. We conclude that intercropping can be an important silvicultural practice to facilitate forest restoration. Multi-purpose shrubby crop species with cropping cycles of more than 6 months are particularly beneficial, as they quickly shade out grasses, thus reducing the need for herbicides.     

Growth and fuelwood properties of five tree and shrub species in the Sahelian and Sudanian ecozones of Mali: relationships with mean annual rainfall and geographical coordinates

In the Sahelian and Sudanian ecozones of West Africa, the rainy season lasts only 3–4 months per year, there are steep rainfall gradients with latitude and longitude, the climate is becoming increasingly hotter and drier with more variable rainfall, some fuelwood species are disappearing locally, demand for fuelwood is increasing, and we expect that fuelwood production will be increasingly limited to drier sites in the future. We need a methodology, therefore, to identify the best species for fuelwood production in drier sites. We used a methodology that could be used to identify the best fuelwood species for drier and wetter sites in regions where there are steep rainfall gradients. We investigated variation in growth and fuelwood properties of five species (Balanites aegyptiaca, Combretum glutinosum, Guiera senegalensis, Piliostigma reticulatum, Ziziphus mauritiana) along rainfall gradients. Growth parameters (tree height, stem diameter under bark, mean ring width) and fuelwood properties (basic density, volatile matter, fixed carbon, ash content, moisture content, gross calorific value, gross calorific value per cubic meter, fuel value index) were adjusted for tree age. For each species, linear regressions were used to determine the effects of mean annual rainfall and geographical coordinates, which were correlated with rainfall, on tree growth and fuelwood properties; and the effects of tree growth on fuelwood properties in lower, intermediate and higher rainfall zones. Geographical coordinates explained more variation than rainfall, and relationships with rainfall differed among species. Larger trees had wood with higher basic density, fixed carbon, ash content, moisture content, gross calorific value and/or gross calorific value per cubic meter, but lower volatile matter and/or fuel value index. Relationships between growth and fuelwood properties were not significant in all species and rainfall zones. Based on this study, we recommend producing fuelwood of C. glutinosum and G. senegalensis.     

Analysis of the relationships among O(3) uptake, conductance, and photosynthesis in needles of Pinus ponderosa

We studied the effects of O(3) uptake on conductance (g(wv)) and photosynthesis (A) in needles of ponderosa pine (Pinus ponderosa Laws.) seedlings exposed for 70 days to one of three O(3) regimes-Low-O(3) (0.1 micro mol mol(-1) daily peak), High-O(3) (0.2 micro mol mol(-1) daily peak), and Low/High-O(3) (alternating 2 days Low-O(3) and 2 days High-O(3)). Seedlings exposed to charcoal-filtered air served as controls. Total O(3) exposures, expressed as ppm-h (the sum of the average hourly concentration in ppm ( micro mol mol(-1)) over the exposure period), were 77, 135, 105 and 4 for the Low-O(3), High-O(3), Low/High-O(3) and control treatments, respectively. Conductance (g(wv)) declined to about 60% of the value in control seedlings by Day 6 in seedlings in the High-O(3) treatment and by Day 37 in seedlings in the Low/High-O(3) treatment, but g(wv) did not decline at all in seedlings in the Low-O(3) treatment. At the end of the 70-day experiment, cumulative O(3) uptake, calculated from measured g(wv) values and assuming an internal O(3) concentration of zero, was 12.2, 13.5, and 14.7 mmol m(-2) for seedlings in the Low-O(3), Low/High-O(3), and High-O(3) treatments, respectively; however, O(3) uptake was reduced by 0, 24, and 36%, respectively, from that expected if there had been no decline in g(wv). With increasing total O(3) exposure, A declined, but the reduction was not strictly cumulative, i.e., A measured on Days 49 and 70 was similar for a given treatment even though both total O(3) exposure and uptake had increased. At the end of the experiment, A at near saturating CO(2) (1000 micro mol mol(-1)) and saturating photosynthetic photon flux density was reduced by about 25, 40 and 50% in seedlings in the Low-O(3), Low/High-O(3) and High-O(3) treatments, respectively, compared to the control seedlings. The ratio of internal to external CO(2) concentrations, an indicator of relative change in stomatal limitation of A, did not change over time and did not differ among treatments, suggesting that A and g(wv) decreased in parallel. After 40-60 days without O(3), A of seedlings in all O(3) treatments was not significantly different. Our data indicate that O(3)-induced stomatal closure was a result of reduced A and that decreased g(wv) reduced O(3) uptake to a rate that needles of ponderosa pine could tolerate without exhibiting further reductions in gas exchange capacity.     

Growth form and seasonal variation in leaf gas exchange of Colophospermum mopane savanna trees in northwest Botswana

We investigated differences in physiological and morphological traits between the tall and short forms of mopane (Colophospermum mopane (Kirk ex Benth.) Kirk ex J. Léonard) trees growing near Maun, Botswana on a Kalahari sandveld overlying an impermeable calcrete duricrust. We sought to determine if differences between the two physiognomic types are attributable to the way they exploit available soil water. The tall form, which was located on deeper soil than the short form (5.5 versus 1.6 m), had a lower leaf:fine root biomass ratio (1:20 versus 1:6), but a similar leaf area index (0.9-1.0). Leaf nitrogen concentrations varied between 18 and 27 mg g(-1) and were about 20% higher in the tall form than in the short form. Maximum net assimilation rates (A sat) occurred during the rainy seasons (March-April 2000 and January-February 2001) and were similar in the tall and short forms (15-22 micromol m(-2) s(-1)) before declining to less than 10 micromol m(-2) s(-1) at the end of the rainy season in late April. As the dry season progressed, A sat, soil water content, predawn leaf water potential (Psi pd) and leaf nitrogen concentration declined rapidly. Before leaf abscission, Psi pd was more negative in the short form (-3.4 MPa) than in the tall form (-2.7 MPa) despite the greater availability of soil water beneath the short form trees. This difference appeared attributable to differences in root depth and density between the physiognomic types. Stomatal regulation of water use and carbon assimilation differed between years, with the tall form having a consistently more conservative water-use strategy as the dry season progressed than the short form.     

Geographic variation in tree growth and wood density of <Emphasis Type="Italic">Guazuma crinita</Emphasis> Mart. in the Peruvian Amazon

Guazuma crinita Mart. is a fast-growing timber tree in the Peruvian Amazon. A trial of 11 Peruvian provenances was established in three planting zones. Tree growth at 6, 12, 18 and 30 months and wood density at 32 months were analyzed across and within zones. Zones accounted for more variation than provenances. Density varied significantly due to provenances in the analysis across zones, whereas growth exhibited strong provenance by zone interactions. The local provenance grew well and produced relatively dense wood in all zones. The relative magnitude of variation due to provenances depended on the planting zone. Density was greater in the lower than in the upper stem. Density increased with longitude from the more humid to the drier part of the sample region. In general, correlations indicated that larger trees had lower density and a larger difference in density between the lower and upper stem, but the strength of these relationships depended on the provenance and zone. Results suggest that fast-growing provenances can be selected at an early age without significantly reducing wood density. The local provenance is recommended for reforestation pending future research demonstrating the superiority of foreign provenances. Some practical implications for tree-improvement programs are discussed.     

Growth and survival of Prosopis africana provenances tested in Niger and related to rainfall gradients in the West African Sahel

Prosopis africana (Guill., Perrott. and Rich.) Taub. is important for farming and pastoralist communities in the West African Sahel. It provides a range of essential products (wood, fuel, food, fodder and medicines) and services (soil fertility improvement), and is one of many native tree species maintained in the traditional agroforesty system, known as the parkland. However, this and other valuable species are disappearing in many parts of the region due to over-exploitation and, perhaps, due to increasingly drier conditions in the region. A provenance/progeny test (275 families from 28 provenances in Burkina Faso and Niger) was established at one location in Niger to investigate geographic variation in the species. Mean annual rainfall decreases from south to north and from west to east in the seed collection area. Given these gradients, one would expect clinal variation in growth and survival of P. africana and other tree species that occur naturally in the region. Tree growth (height, number of primary branches, stem diameter at 20 cm and 1.3 m) and survival were analyzed at 11 years. Variation due to provenances was significant for growth variables and almost significant for survival. Variation due to families within provenances was not significant for any variable. Provenance means for growth and survival increased from the more humid to the drier parts of the sample region. The clines in growth and survival with regional rainfall patterns suggest that tree improvement and conservation programs should collect seeds from populations in the drier zones for planting and conservation in the West African Sahel. This may be particularly important when one considers the trend in global warming.     

Intracellular infection by fungi in mycorrhizae of damaged spruce trees

Intracellular infection was found in the vascular tissue and meristem of mycorrhizae of a severely damaged stand of spruce and fir trees in the Northern Black Forest. Two fungal species could be isolated from surface sterilized mycorrhizae: Mycelium radicis atrovirens and Cryptosporiopsis cf. abietina. Infection tests with spruce seedlings revealed that mainly Cryptosporiopsis cf. abietina must be held responsible for infection of the vascular tissue. Less infectious was Mycelium radicis atrovirens, which infected only cortex cells.     

Identification of a molecular marker linked to an Agropyron elongatum-derived gene Lr19 for leaf rust resistance in wheat

The leaf rust resistance gene Lr19, transferred from Agropyron elongatum into wheat (Triticum aestivum L.) imparts resistance to all pathotypes of leaf rust (Puccinia recondita f.sp. tritici) in South‐east Asia. A segregating F₂ population from a cross between the leaf rust resistant parent ‘HW 2046’ carrying Lr19 and a susceptible parent ‘Agra Local’ was screened in the phytotron against a virulent pathotype 77‐5 of leaf rust with the objective of identifying the molecular markers linked to Lr19. The gene was first tagged with a randomly amplified polymorphic DNA (RAPD) marker S73₇₂₈. The RAPD marker linked to the gene Lr19 which mapped at 6.4 ± 0.035 cM distance, was converted to a sequence characterized amplified region (SCAR) marker. The SCAR marker (SCS73₇₁₉) was specific to Lr19 and was not amplified in the near‐isogenic lines (NILs) carrying other equally effective alien genes Lr9, Lr28 and Lr32 enabling breeders to pyramid Lr19 with these genes.