全文获取类型
收费全文 | 106篇 |
免费 | 9篇 |
专业分类
林业 | 9篇 |
农学 | 3篇 |
基础科学 | 1篇 |
14篇 | |
综合类 | 7篇 |
农作物 | 4篇 |
水产渔业 | 23篇 |
畜牧兽医 | 48篇 |
园艺 | 1篇 |
植物保护 | 5篇 |
出版年
2023年 | 2篇 |
2022年 | 3篇 |
2021年 | 3篇 |
2020年 | 5篇 |
2019年 | 6篇 |
2018年 | 5篇 |
2017年 | 2篇 |
2016年 | 6篇 |
2015年 | 7篇 |
2014年 | 13篇 |
2013年 | 5篇 |
2012年 | 5篇 |
2011年 | 5篇 |
2010年 | 3篇 |
2009年 | 5篇 |
2008年 | 5篇 |
2007年 | 6篇 |
2006年 | 6篇 |
2005年 | 4篇 |
2004年 | 4篇 |
2003年 | 7篇 |
2002年 | 4篇 |
2001年 | 1篇 |
2000年 | 3篇 |
排序方式: 共有115条查询结果,搜索用时 0 毫秒
81.
A tumor within the right globe in a 9-year-old Shetland sheepdog was examined pathologically. The tumor was composed of spindle or oval cells arranged in interwoven bundles with intervening collagenous or mucinous matrices. Immunohistochemically, the tumor bound antibody directed to S-100 protein and vimentin, but not to desmin, actin smooth muscle, or neurofilament. Electron microscopy revealed that the tumor cells had poorly developed cytoplasmic processes, desmosomes between closely contiguous cells, and a discontinuous basement membrane-like material. Based on these findings, the tumor was diagnosed as a peripheral nerve sheath tumor (PNST) histologically. To the authors' knowledge, this is the first reported case of intraocular PNST in dogs. 相似文献
82.
Ishii M Kobayashi S Acosta TJ Miki W Yamanoi T Matsui M Miyake Y Miyamoto A 《The Journal of reproduction and development》2008,54(4):270-274
The aim of this study was to clarify the relationship between circulating oxytocin (OT) and PGF(2alpha) metabolite (PGFM) in mares at the third stage of labor and placental expulsion time in order to investigate a cause of retained placenta of which the incidence increase in a heavy draft mare. Blood was sampled every 5 min from foaling to expulsion of the placenta in 18 heavy draft mares to evaluate circulating OT and PGFM. The relationships between OT and PGFM concentration and recorded placental expulsion times were investigated. The results were as follows (1) The highest level of OT concentration was observed close to foaling in 15 mares. (2) The OT concentrations close to foaling were variable with a large difference from the lowest concentration, 22.1 pg/ml, to the highest concentration, 209.3 pg/ml. (3) The highest level of PGFM was observed close to foaling in 17 mares. (4) During the 60 min following foaling, the OT concentrations of the mares (n=11) that had a shorter placental expulsion time (i.e., <1 h), were significantly higher than those of the mares (n=7) that had a longer placental expulsion time (i.e., >1 h; P<0.05). Collectively, the OT concentration immediately after foaling is negatively related to the placental expulsion time. Deficiency of OT secretion at foaling have should be considered as one of the causes of retained placenta in heavy draft mares. 相似文献
83.
Yusuke Komiya Toshiya Nakamura Momoko Ishii Kuniyoshi Shimizu Eri Hiraki Fuminori Kawabata Mako Nakamura Ryuichi Tatsumi Yoshihide Ikeuchi Wataru Mizunoya 《Animal Science Journal》2019,90(6):781-789
Skeletal muscle fiber is largely classified into two types: type 1 (slow‐twitch) and type 2 (fast‐twitch) fibers. Meat quality and composition of fiber types are thought to be closely related. Previous research showed that overexpression of constitutively active peroxisome proliferator‐activated receptor (PPAR)δ, a nuclear receptor present in skeletal muscle, increased type 1 fibers in mice. In this study, we found that hexane extracts of Yamabushitake mushroom (Hericium erinaceus) showed PPARδ agonistic activity in vitro. Eight‐week‐old C57BL/6J mice were fed a diet supplemented with 5% (w/w) freeze‐dried Yamabushitake mushroom for 24 hr. After the treatment period, the extensor digitorum longus (EDL) muscles were excised. The Yamabushitake‐supplemented diet up‐regulated the PPARδ target genes Pdk4 and Ucp3 in mouse skeletal muscles in vivo. Furthermore, feeding the Yamabushitake‐supplemented diet to mice for 8 weeks resulted in a significant increase in muscle endurance. These results indicate that Yamabushitake mushroom contains PPARδ agonistic ligands and that dietary intake of Yamabushitake mushroom could activate PPARδ in skeletal muscle of mice. Unexpectedly, we observed no significant alterations in composition of muscle fiber types between the mice fed control and Yamabushitake‐supplemented diets. 相似文献
84.
Akihide Kasai Wataru Sakamoto Yasushi Mitsunaga & Shotaro Yamamoto 《Fisheries Oceanography》2000,9(3):259-270
The long-term behaviour of immature yellowtails, Seriola quinqueradiata , was studied using electronic data-recording tags in Sagami Bay, Japan, during the autumn and early winter. Temperature or depth data were continuously recorded for 5–69 days on six yellowtails. The temperature recorded from the 0-year-old yellowtails showed only small variations (<0.5°C day−1 ) around the SST on the coast, indicating the 0-years stayed in the immediate coastal area with little horizontal movement. The 1-year-old yellowtail showed larger temperature variations that reached 3°C day−1 , which implies distinctly larger horizontal movements than the 0-year-olds. Depth records showed the 0-years stayed around 10 m while the 1-year yellowtail swam in deeper water (mainly 10–30 m) with large variations. The latter often dived to 40 m in the daytime, but shallower than the depth of the thermocline. Both temperature and depth records showed apparent diurnal patterns. Comparing the fish data with environmental conditions, it is suggested that 1-year yellowtails stay close to the coast during the night, move at dawn to feed in the daytime and return at dusk via a frontal zone. In addition, wind stress could change yellowtails' behaviour. 相似文献
85.
Liyan Wang Liming Bai Daisuke Tokunaga Yusuke Watanabe Toshiaki Hasegawa Jun-ichi Sakai Wanxia Tang Yuhua Bai Katsutoshi Hirose Takao Yamori Akihiro Tomida Takashi Tsuruo Masayoshi Ando 《Journal of Wood Science》2008,54(5):390-401
Twelve basic taxoids and 22 neutral taxoids were isolated from basic and polar neutral fractions of the extracts of needles
and twigs of Taxus cuspidata. Among them, taxine NA-13, 3,11-cyclotaxinine NN-1, taxinine NN-6, 11(15→1)abeo-taxinine NN-1, taxine NA-8, and taxine NA-4 were isolated first from natural sources by us. The cytotoxic activity of isolated
compounds was evaluated against three human cell lines: normal human fibroblast cells (WI-38), malignant tumor cells induced
from WI-38 (VA-13), and human liver tumor cells (HepG2). 7-Epitaxol, 7-epicephalomannine, taxinine NN-6, taxine NA-2, taxuspine
H, and taxagifine were active toward VA-13 cells and 7-epitaxol, 7-epicephalomannine, taxinine NN-1, 9,10-deacetyltaxinine,
and taxagifine were active toward HepG2 cells. The multidrug-resistant (MDR) cancer reversal activity of isolated compounds
was evaluated on the basis of the amount of vincristine (VCR) accumulated in MDR human ovarian cancer 2780 AD. Taxine NA-8,
taxine NA-2, 5-cinnamoyl-10-acetyltaxicin II, and taxinine NN-1 indicated stronger MDR cancer reversal activity than verapamil.
The result of primary screening based on 39 human cancer cell lines suggests that taxinine NN-1 belongs to a new mechanistic
class and is a new anticancer agents. 7-Epicephalomannine was found to be an effective anticancer agent with tubulin as its
molecular target, which is the same as paclitaxel.
Part of this work was presented at the 74th Annual Meeting of the Chemical Society of Japan, Tanabe, March 1998, and the 41st
Symposium on the Chemistry of Terpenes, Essential Oils, and Aromatics, Gifu, December 1998. The results of this work were
also presented as patent documents at PCT Int: Appl WO 2001007040 A1, February 2001; Appl WO 2000-Jp 5036, 27 July 2000. Priority:
JP 1999-214273, 28 July 1999; JP 1999-224652, 6 August 1999; JP 2000-76404, 14 March 2000 相似文献
86.
Wataru Mitsuhashi Shoji Asano Kazuhisa Miyamoto Sanae Wada 《Pest management science》2014,70(1):46-54
87.
Takahiro SUZUKI Hideyuki TAKAISHI Tomowa SAKATA Mai‐Khoi Q. DO Minako HARA Akiko SATO Wataru MIZUNOYA Takanori NISHIMURA Akihito HATTORI Yoshihide IKEUCHI Ryuichi TATSUMI 《Animal Science Journal》2010,81(2):245-251
Satellite cells, resident myogenic stem cells found in postnatal skeletal muscle, are most abundant during early postnatal development and sharply decline in frequency thereafter to adult levels in mice and rats. Therefore, postnatal changes in satellite cell mitotic activities are important aspects for further understanding a muscle growth strategy. In large meat‐production animals, however, the traditional in vivo proliferation assay may be less realistic because it requires intra‐peritoneal (ip) injection of huge dosage of mutagenic nucleosides, 3H‐labeled thymidine or bromodeoxyuridine (BrdU), at each age‐time of sacrifice. We report in the present pilot study using rats that in vivo proliferation activity of satellite cells can be evaluated by an in vitro BrdU‐incorporation assay in early cultures. Briefly, satellite cells were prepared from upper hind‐limb and back muscles and maintained for 24 h with imposing by BrdU addition for the last 2 h, followed by the regular immunocytochemistry for determining BrdU‐incorporated cell percentage. This in vitro assay demonstrated a rapid decrease in proliferating satellite cell frequency to the adult level during about 3‐month period after birth, and yielded a high correlation to the measurements by the in vivo BrdU ip‐injection method during the postnatal period examined from day‐2 to month‐11. The in vitro proliferation assay may be further adaptable for large domestic animals by the combination with a muscle biopsy technique that enables age‐interval sampling from the same growing animals. 相似文献
88.
Takahiro Iizuka Tsutomu Kuboyama Wataru Marubashi Masayuki Oda Takahiro Tezuka 《Euphytica》2012,186(2):321-328
To elucidate the genetic mechanism of hybrid lethality observed in hybrids between cultivated tobacco, Nicotiana tabacum, and wild tobacco species in the section Suaveolentes, genetic analyses were conducted through the triple cross of the hybrids of wild species, including N. benthamiana and N. fragrans, and N. tabacum. N. benthamiana and N. fragrans produced only viable hybrids after crossing with N. tabacum. Subsequently, N. benthamiana and N. fragrans were crossed with N. africana, N. debneyi, and/or N. suaveolens, which produced inviable hybrids after crossing with N. tabacum. Hybrids of the wild species were obtained from four of the six cross combinations. Only when hybrid plants of N. debneyi × N. fragrans, whose hybridity was confirmed by morphological characteristics, random amplified polymorphic DNA analysis, and chromosome observation, were crossed with N. tabacum, triple hybrids were obtained and segregated 1:1 (lethal:viable). Based on these results, a single dominant gene, designated Hybrid Lethality A1 (HLA1), in N. debneyi was found to control hybrid lethality by the interaction with gene(s) on the Q chromosome in N. tabacum. This represents the first report to identify a causal gene for hybrid lethality in the genus Nicotiana. 相似文献
89.
Yamanaka T Tsujimura K Kondo T Yasuda W Okada A Noda K Okumura T Matsumura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(3):293-295
Japanese encephalitis (JE) developed in an unvaccinated half-bred horse kept in Tottori Prefecture, Japan. The animal showed ataxia with pyrexia and low appetite, and ultimately died. A viral strain was isolated from the cerebrum of the horse and was identified as JE virus (JEV) by RT-PCR using JEV specific primers. The isolated JEV was classified into genotype I by nucleotide sequence analysis of the viral envelope gene. We believe that this is the first report of the genotype I strain being isolated from a horse. 相似文献
90.
Wataru YAMAMOTO Yasuhiro ITANO Tsunefumi KOBAYASHI Daishiro MIURA Yoshinori KASAHARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):77-82
Human immunoglobulin G (IgG) concentrates are immune-modulating,
anti-inflammatory plasma-derived products. Clinical studies in recent years have suggested
that IgG attenuates neuropathic pain. In this study, effects of sulphonated IgG on the
development and maintenance of a mechanical allodynia-like response were examined in mice
with neuropathic pain induced by a partial sciatic nerve ligation (PSL). When sulphonated
IgG (400 or 1,000 mg/kg/day, i.p.) was administered for 5 days, from 1 day before surgery
to post-operative day (POD) 3, the development of a mechanical allodynia-like response was
attenuated. On the other hand, sulphonated IgG had little effect on the maintenance of a
mechanical allodynia-like response when administered for 5 days, from POD 11 to POD 15, at
which time a mechanical allodynia-like response had already been developed. To explore the
mechanism of sulphonated IgG, the mRNA expression of inflammatory cytokines was evaluated
in the injured sciatic nerve. Sulphonated IgG (1,000 mg/kg/day, i.p.) that was
administered for 3 days, from 1 day before surgery to POD 1, significantly attenuated the
up-regulation of tumor necrosis factor-α and monocyte chemotactic protein-1 mRNAs on POD
1. These results suggest that prophylactic treatment with sulphonated IgG attenuates the
development of mechanical allodynia-like response by inhibition of inflammatory cytokine
expression in mice with PSL. 相似文献