绢丝昆虫茧丝蛋白酶抑制剂含量的比较分析

以 6种绢丝昆虫的茧壳为材料 ,对茧丝蛋白酶抑制剂的含量及热稳定性进行了研究。结果表明 :家蚕品种间茧丝蛋白酶抑制剂的含量差异极显著 ,外层丝中含量较多 ,F1代的含量呈双亲中间型 ,偏向于含量较多的亲本 ,雌雄间含量无显著差异 ;家蚕茧丝蛋白酶抑制剂对热稳定性较强 ;天蚕绿茧、野蚕的茧丝中有少量的蛋白酶抑制剂存在 ,蓖麻蚕的茧丝中有微量蛋白酶抑制剂存在 ,天蚕黄茧、柞蚕茧、樗蚕茧的茧丝中基本无蛋白酶抑制剂存在。     

降低河蟹育苗成本的几种方法

随着河蟹育苗技术的日趋稳定及苗种生产规模的不断扩大，河蟹苗种供大于求的局面正由北向南扩大。蟹苗销售难的状况导致蟹苗价格下跌，蟹苗生产如不及时调整技术，压缩成本，将面临亏损的威胁。笔者近几年在育苗技术服务时，在降低育苗成本方面做了一些积极的探索，并取得了明显效果，现总结如下： １．培养好基础饵料。 当今河蟹育苗生产一般有清水布苗和肥水布苗两种方法。肥水布苗即通过培养单胞藻类基础饵料，可以节省幼体饵料的投喂量，尤其是螺旋藻粉等价格较高的前期饵料，降低饵料成本。具体做法是：在布苗前３～６天，向育苗池中蓄…     

鹅出血性坏死性肝炎的初步研究

本病为雏鹅的一种病毒性传染病，病毒为球形，无囊膜，直径为76－86nm大小的颗粒，属于呼肠孤病毒科鹅呼肠孤病毒。能在发育良好的鹅胚等禽胚内复制，致死胚胎，并具有特征性病变。患病雏鹅以出血性坏死性肝炎为特征性的大体病理变化和显微病理变化。     

水资源核算及对GDP的修正——以中国东部经济发达地区为例

对中国东部经济发达地区水资源核算的结果表明 ,以GDP为主要衡量指标的现行国民经济核算体系过高地估计了该地区经济的发展水平。扣除水资源价值损失 ,1994～ 1997年区域GDP平均下降 5 5 8% ,这意味着东部经济发达地区经济增长存在着较为严重的环境负债     

The failure of selenium supplementation to prevent copper-induced liver damage in Fischer 344 rats.

This study evaluates the ability of selenium (Se) supplementation to prevent experimental copper (Cu)-induced hepatocellular damage. Weanling male Fischer 344 rats were randomly assigned to groups of 15, 3 groups (A,B,C) were fed Cu-loaded diets (containing 2000 microg/g copper, added as CuSO4) and different levels of Se (added as Na2SeO3 x 5H2O) as follows: A) Cu-loaded/Se adequate diet (0.4 microg/g Se, fed basis); B) Cu-loaded/Se-supplemented diet (2 microg/g Se); and C) Cu-loaded/Se-deficient diet (< 0.2 microg/g). Three additional groups (D,E,F) were fed diets containing adequate levels of Cu (14 microg/g Cu, fed basis) and different levels of Se as follows: D) Cu-adequate/Se-adequate diet; E) Cu-adequate/Se-supplemented diet (2 microg/g Se); and F) Cu-adequate/Se-deficient (< 0.2 microg/g) diet. After 4, 8, and 12 weeks on the experimental diets, liver samples were processed for histology, histochemistry, metal analysis, glutathione peroxidase (GSH-Px) measurement, and quantification of malondialdehyde (MDA). Morphologic changes characteristic of Cu-associated hepatitis, without an increase in hepatic MDA levels, were seen in all Cu-loaded rats in each sampling. Similar changes occurred in rats fed Se-adequate, Se-supplemented and Se-deficient diets. This study demonstrates that Fischer 344 rats fed 2000 microg/g Cu develop morphologic changes due to Cu toxicity without evidence of lipid peroxidation. Furthermore, Se supplementation does not result in protection against Cu-induced liver injury.     

埋地管道新型预应力抗滑桩的应用

介绍了埋地输油管道新型预应务抗滑桩的构成和抗滑原理，并对其受力状况进行了分析。以某油加库外滑坡地段埋地输油管道的实际应用为例，表明采用新型预应力锚索抗滑桩保护滑坡地段的埋地输油管道不仅是可行的，而且具有较好的经济性、实用性和可靠性。     

再论新形势下的乡镇企业

乡镇企业如何走出目前的经营困境，如何确定自己的战略选择，不同的人会有不同的认识。笔者试图从经营理念和管理者收购这一“软”一“硬”两方面做一些探讨。     

Mycoplasma hyopneumoniae colonization of pigs sired by different boars

Differences in Mycoplasma hyopneumoniae colonization were evaluated in experimentally inoculated pigs sired by 3 different boars of the same genetic line. Forty-six pigs were used, including a treatment group and positive and negative control groups. The pigs were intratracheally inoculated with an M. hyopneumoniae suspension or with Friis media as a placebo. To evaluate differences in the magnitude of colonization during a 35-day period, nasal and tracheal swabs were collected weekly and tested by nested polymerase chain reaction (N-PCR). Temperature, weight and circulating antibodies were measured for 35 days. At 11 and 35 d postinoculation the pigs were necropsied and macroscopic and microscopic lesions were determined. A section of bronchus was tested by the indirect immunofluorescence test (IFAT), scanning electron microscopy (SEM) and N-PCR. The N-PCR results from the nasal and tracheal swabs showed that the pigs sired by one boar (B3) had a distinctive colonization pattern, different from that of the pigs from the other 2 boars and from the positive controls. SEM studies demonstrated that at 35 d postinoculation a higher proportion of B3 pigs had lower numbers of mycoplasmas attached to the cilia compared with B1 and B2 offspring. No significant differences were observed in temperature and weight gain among groups by ANOVA; however, with use of a 2 × 2 table, temperature differences were observed between pigs sired by boars B1 and B2 at 4 d postinoculation. No pigs seroconverted, showed gross or microscopic lesions, or had positive IFAT results. These results provide evidence of differences in patterns of colonization between pigs sired by different boars, suggesting a possible genetic effect.     

影响烟草花粉胚胎发生的某些因子——铁盐、蔗糖和腺素

本文研究了铁盐、蔗糖等培养基成份对烟草花粉胚胎形成的影响。结果表明,铁盐和蔗糖与烟草花粉胚形成有密切关系。培养基中没有铁盐,胚发育停止球形期;没有蔗糖、胚发育受阻在多细胞原胚期,不能进一步发育。培养基中仅有蔗糖,胚发育停止在鱼雷胚早期,将其转到只含铁盐的培养基中,鱼雷胚可继续发育直至成株。培养基中不附加铁盐或蔗糖,胚的发育均受阻。只有铁盐和蔗糖而无大量元素、微量元素及有机成份,即能形成胚状体并再生植株,但胚胎发生频率低,表明其它附加成份并不是没有意义的。另外,培养基中附加一定浓度的腺素对花粉胚形成是有利的,但高浓度是有害的。     

荒漠绿洲区紫花苜蓿生产技术的研究

荒漠绿洲区的紫花苜蓿在传统的生产管理基础上，提出了苜蓿生产、管理及加工利用的科学技术及措施，并展望了苜蓿产业化发展的市场前景。