Production and identification of monoclonal anti-idiotypic antibody against uterine cervical cancer

AIM: To produce monoclonal anti-idiotypic antibody against uterine cervical cancer and identify its antigenic mimicry properties. METHODS and RESULTS: Mouse splenocytes were immunized in serum-free medium containing immunoactive reagents by using monoclonal antibody AU14-1 (Ab1) recognizing common epitopes shared with both mouse and human uterine cervical cancer-associated antigen molecule as immunogen. The in vitro immunized lymphocytes were fused with murine myeloma SP2/0. Hybridoma cell line secreting monoclonal anti-idiotypic antibody (Ab2) was established by screening and cloning. The identification of antigenic mimicry properties of Ab2 with ELISA, combinding and competing inhibition assays, and immunohistochemistry staining showed that this monoclonal anti-idiotypic antibody was Ab2β, which carried an “internal image” of cervical cancer cell membrane surface antigen. CONCLUSION: A monoclonal anti-idiotypic antibody with an “internal image” of cervical cancer antigen was obtained.     

植物汁液化学组成及其与菜心抗性和Cd累积的关系

Sap mixtures of the xylem, phloem, and vacuoles from low and high Cd accumulator varieties of Brassica parachinensis L. H. Bailey were analyzed under Cd stress to understand the biochemical mechanisms of Cd accumulation in plants. Low Cd accumulator （‘Teqing-60＇） and high Cd accumulator （‘Chixin-2＇） plants were grown in Cd-treated soil in pots in a greenhouse. Percentage of cell wall-bound Cd was estimated, pH level and the concentrations of amino acids, organic acids, anions, and cations in both stem and root saps were determined for the calculation of Cd speciations using the computer program GEOCHEM. The results showed that ‘Teqing-60＇ had a significantly higher （P ≤ 0.05） percentage of Cd bound to cell walls in roots and a significantly higher （P ≤ 0.05） pH in the root sap. ‘Teqing-60＇ also contained a higher concentration of total amino acids in both roots and stems compared with the high Cd accumulator variety ‘Chixin- 2＇. However, between the two accumulators, for stems and for roots, there were no significant differences in non-amino organic acids. GEOCHEM calculations showed that Cd in the root sap of ‘Teqing-60＇ mainly combined with amino acids, especially alanine. Compared with ‘Chixin-2＇, in the root sap of ‘Teqing-60＇, much lower levels of Cd as free ions or bound to simple ligands were found, indicating that less ‘Teqing-60＇ is transferred to stems and leaves. Cadmium activity in the shoot sap of ‘Teqing-60＇ was much lower than that in ‘Chixin-2＇; therefore, ‘Teqing-60＇ exhibited higher Cd resistance. However, direct determination of the Cd complexes from xylem and phloem sap is needed to verify these results.     

Recovery of 15N-labelled fertilizer in crops, drainage water and soil using monolith lysimeters in south-east Nigeria

Labelled urea was applied to monolith lysimeters in the 1st year of a 2-year experiment at Onne in south-east Nigeria. On eight lysimeters maize and rice were grown in each of the 2 years. Four lysimeters were similarly cropped in the 2nd year after being uncropped in the 1st year. Measurements were made over the 2-year period of labelled and unlabelled mineral nitrogen in the drainage water, and labelled and unlabelled nitrogen in the crops. At the end of the experiment, weeds and the soil were also analysed for labelled and unlabelled nitrogen.
The total recovery of ¹⁵N in crop, soil and leachate varied between 70 and 93%. It was lowest when applied to the second season rice crop, which recovered only 15%, and highest when it was leached in the 1st year or was taken up by the maize crop. The highest crop uptake was 31%. The results indicate that, depending on the treatment, between 10 and 30% of the ¹⁵N was immobilized in the soil, lysimeters cropped in the 1st year lost between 22 and 29% of the ¹⁵N in drainage water, and between 7 and 30% was lost by denitrification. The accuracy of these figures is discussed.     

福建花生地膜覆盖栽培技术应用效果初报

试验结果表明，覆膜（专用膜）栽培花生表现出单株分枝多、鲜蔓重、饱果数多且荚果大的优势，与未覆膜之间的产量差异达极；除草膜与光解膜产量差异不明显，但与普通地膜和不盖膜的差异均达极显著；覆膜早播的与其它处理的产量差异也均达极显著，其主茎高、侧枝长、总分枝数、单株结果数等均比迟播的具有明显优势。     

中草药免疫增强剂的药效学和毒理学试验研究

"菌毒克星"免疫增强剂是一种动物用纯中草药制剂.为探讨其药效学与毒理学作用,进行了抑菌、解热、止咳平喘、血液学免疫指标和毒理学检测.结果表明,该制剂具有明显的抗菌、退热和止咳平喘作用;血液学检查显示中草药组各项免疫指标高于对照组;毒性试验说明用过此药的试验动物其体重、器官、脏器系数同用肉眼观察的结果均无明显的差异或异常,由此说明"菌毒克星"免疫增强剂是一种高效、安全的中草药制剂.     

A geminivirus causing vein yellowing of Ageratum conyzoides in Singapore

A vein-yellowing disease of Ageratum conyzoides in Singapore was shown to be caused by a geminivirus, here named ageratum yellow vein virus (AYVV), which was transmitted by the whitefly Bemisia tabaci but not by inoculation with sap or through seed. AYVV particles (30 × 20 nm) are serologically related to those of other whitefly-transmitted geminiviruses, and reacted with some monoclonal antibodies elicited by particles of African cassava mosaic or Indian cassava mosaic geminiviruses. However, the epitope profile of AYVV differed from the profiles of these viruses, and from those of geminiviruses from vein yellowing-affected A. conyzoides from India and from yellow leaf curl-affected tomato from either Singapore or India. The results provide further evidence of antigenic differences among geminiviruses that cause similar diseases in the same plant species in different geographical regions.     

The Impact of Tourism on Local Government Expenditures

The aim of this study is to broaden the understanding of the impact of tourism on local government expenditures. Specifically, a regression model is developed to examine the hypothesis that there is a direct relationship between the degree of reliance of the local economy on tourism and local government expenditures. This study indicates that the degree of reliance of a local economy on tourism does have a statistically significant impact on the level of capital outlays, transportation, police protection, fire protection, corrections, parks and recreation, financial administration, and general government administration expenditures. This analysis indicates that the share of tourism in the local economy can influence expenditures on a variety of local government services, thus tourism should not be regarded as a totally costless instrument of economic development.     

甘薯脱毒苗生产力表现研究

试验研究了金山57和岩薯5号脱毒苗产量形成特性,结果两个品种脱毒苗比对照增产分别达显著和极显著水平,生物产量和经济系数均高于对照。     

Sources of Verticillium fungicola on a commercial mushroom farm in England

From 1979 to 1981 Verticillium fungicola, a pathogen to Agaricus bisporus, was detected in 47 out of 485 samples of baled peat and in one out of 126 samples of bagged limestone arriving at an isolated mushroom farm in the UK. The pathogen was not detected in 875 samples of water, soil, mushroom spawn or spawned compost. Casing (a mixture of peat and limestone) prepared and used on the farm contained detectable V. fungicola in 25 out of 108 samples. The primary sources of V fungicola were thus the two constituents of casing, especially peat.
Secondary sources of V. fungicola were numerous and included symptomless and diseased mushrooms, pickers and other staff, floors and doors and the principal pests (sciarid flies and mites).
Viable airborne spores of V. fungicola were detected with an Andersen sampler in farm buildings at all stages in crop production. The spore load increased with the age of the crop. Few airborne spores were detected immediately outside affected buildings, and very few at the farm entrance 30 m away. There was no evidence that V. fungicola reached the farm from the surrounding air. despite substantial soil disturbance nearby.
Selective media for V. fungicola are described.