The effects of habitat connectivity on cryptogam richness in boulder metacommunity

We investigated the effects of habitat patch size and connectivity on cryptogam richness on 288 calcareous boulders. The studied boulders were found to be a particularly suitable ‘metacommunity model system’ and allowed comparison of species richness among cryptogams with differing substrate specificity, life history and red list status. The dependence of species richness on boulder surface area was modelled using Arrhenius power model, and contrasts were analysed between connected and isolated boulders. Species richness was clearly related to boulder size, but the rates of increase of species richness with increasing boulder area (z-values) were highly variable among species groups. Highest value of z (1.17) was found for species with large propagules, and values around 0.5 were obtained for calciphilous and regionally red-listed species and z = 0.42 for perennials. The lowest value of z (0.27) occurred in calcicolous species while for all species, colonists, and species with small propagules the values were 0.3-0.4. The richness of all species was 11% higher on connected than isolated boulders, while connected boulders had 31% more of both calcicolous and regionally red-listed species and 19% more of colonists. The result suggests that in the investigated system that boulder size is a crucial determinant of cryptogam richness, and their spatial configuration in terms of connectivity may further contribute to cryptogam richness. The latter result lends support to the importance of metacommunity processes for the maintenance of cryptogam richness.     

Evaluation of a diagnostic microarray for the detection of major bacterial pathogens of potato from tuber samples

Potato can be infected with many bacterial pathogens, the detection of which is necessary in seed certification. In this study, a diagnostic microarray previously tested for specificity of probes for detecting the potato bacteria causing blackleg and soft rot (Pectobacterium atrosepticum, Pectobacterium carotovorum, and Dickeya spp.), ring rot (Clavibacter. michiganensis subsp. sepedonicus), scab (Streptomyces scabies and Streptomyces turgidiscabies) and brown rot (Ralstonia solanacearum) from pure culture was evaluated for analytical sensitivity when testing directly from tuber samples. The microarray readily detected all the bacterial species when 100 ng of the target bacterial DNA from pure culture was mixed with DNA from soil microbes and potato. However, detection was inconsistent when total DNA isolated directly from infected tubers or enriched bacterial culture was used. While the high specificity of the probes could be confirmed from the results of the DNA cocktail experiment used as a control, the study demonstrated that the level of analytical sensitivity of the microarray under the tested condition was not sufficient to detect bacteria directly from tubers. Therefore, in addition to the cost and organizational complexities, the low analytical sensitivity and limited reproducibility of the microarray are constraints for establishing the platform for routine detection of potato bacterial pathogens from tuber samples.     

Post-harvest Evaluation of Nitrogen Sufficiency for Small-grain Cereals by Measuring Grain Protein Concentration

Nitrogen (N) fertilizer is an important and expensive input in small-grain cereal production, and growers therefore should aim to optimize its use. Possibilities for using grain protein concentration for post-harvesr evaluation of N sufficiency were determined in this study. Field experiments including spring wheat ( Triticum aestivum L.), spong barley ( Hordeum vulgare L) and spring oats ( Avena sativa L.), and various rates of N fertilizer application were conducted in southern and western-Finland over 2 years. Grain yield and grain protein were positively correlated and firred quadratic regression models. Both critical and optimum levels for grain protein concentration were determined by Cate-Nelson analysis Critical values were 12.2 for wheat, 10.2 for barley and 10.9 for oats, and corresponding optimum values were 13.3, 11.1 and 12.7, respectively. The accuracy of the method was tested using results from on-farm spring wheat trials. The results indicated that N fertilizer uptake and grain yield were best in held where gram protein concentration exceeded the critical values but not the optimum. Growers should use more intensive N fertilization management if grain protein concentration does not exceed critical values. Grain protein concentrations above optimum values indicate over-fertilization for maximum grain yield. Analysing previous research data to identify the "critical level" of grain protein concentration is not difficult, and will provide powers, extension personnel, and fertilizer dealers with a cost effective means of evaluating the efficiency of N use by the crop and for developing N fertilization recommendations.     

Complete atrioventricular canal defect in a foal: Clinical and pathological features

Atrioventricular (AV) canal defects comprise a rare category of congenital heart disease associated with abnormal development of the endocardial cushions. These anomalies include a broad spectrum of lesions involving the atrial septum primum, the inlet portion of the ventricular septum, and the atrioventricular valves. In severe cases heart failure may result. The present report describes cardiopulmonary findings in a six-week-old, female Thoroughbred foal with persistent tachypnea and tachycardia since birth. Clinical, echocardiographic, cardiac catheterization, and pathologic findings were compatible with complete AV canal defect with bi-directional shunting, congestive heart failure, and pulmonary hypertension. Gross cardiac pathologic findings included a large atrioventricular septal defect, a common atriovalvular orifice, five atrioventricular leaflets, with two free-floating bridging leaflets. Histopathologic lesions in the lung included hypertrophy of the pulmonary arteriolar walls due to thickening of the tunica media. This represents a well documented case of complete endocardial cushion defect with anomalous development of the atrioventricular valves and resultant pulmonary hypertension and heart failure.     

Milk production of cows on protein-free feed

The synthesis of bacterial protein in the rumen of lactating cows fed on purified carbohydrates, with urea and ammonium salts as the sole sources of nitrogen, can be increased, through feed adaptation, to a level adequate not only for the maintenance of the cow but also for a relatively high milk production. The best annual milkyield per cow on the experimental feedhas, so far, been 4217 kilograms, calculated as standard milk (684 kilocalories per kilogram of milk). The composition of the test milk is similar to that of normal fat- and proteinrich milk. Fractionation of casein and serum proteins of test milk and normal milk by different methods demonstrated the similarity of the proteins of the two milks. The normal or higher concentrations of the water-solublevitamins in test milk show that the biosynthesis of these vitamins in the rumen is vigorous. The flavor of the two milks is very similar-proof that biosynthesis of the effective flavor compounds of milk occurs in the body of the cow. The only component of test milk whose composition, when small amounts of vegetable oil have been fed, has differed from that of normal milk is the fat. The studies of milk production on the experimental feed have opened up new possibilities for investigating the biosynthesis of different milk components. The studies are also of practical importance: Since the vigorous biosynthesis of proteins from simple nitrogen compounds in the rumen of test-feed-adapted cows has been demonstrated, there are greater possibilities for studying the replacement of protein by urea. In countries where there are plenty of forests, part of the feed of cows can be made up of certain wood products-for instance, hemicellulose and cellulose of low quality. The new findings may also be of value in the dry areas of the globe, where milk would be of vital significance for improving the nutrition of the population.     

Physiological responses to continuous swimming in wild salmon (Salmo salar L.) parr and smolt

Wild salmon (Salmo salar) parr and smolt were forced to swim against constant flow (50 cm.s^–1) for 8 hours. Physiological properties describing the hormonal status, the energy metabolism and the ionic and osmotic balance of fish were measured from the fish prior to and at the end of the swimming test.Plasma cortisol levels were elevated in response to enforced swimming; the response of the smolt was clearly greater than that of the parr. Plasma thyroxine concentration increased in the parr but stayed at the initial level in the smolts. The parr consumed much of their coelomic fat, but the glycogen stores stayed nearly constant. The smolts had very low fat stores, and the glycogen stores were depleted in the test. The ionic and osmotic balance of the parr was stable in the test, but in smolts, the plasma Cl^–1 and osmotic concentrations decreased and muscle moisture increased.The results indicate that downstream migration smolts have markedly lower physiological capacity for continuous swimming than parr.     

Occurrence and survival of potato scab pathogens (Streptomyces species) on tuber lesions: quick diagnosis based on a PCR-based assay

A time-saving and cost-effective polymerase chain reaction (PCR)-based method was developed for species-specific detection of the scab pathogens ( Streptomyces scabies and S. turgidiscabies ) prevalent in potato ( Solanum tuberosum ) in northern Scandinavia. Species specificity of primers was verified using a collection of previously characterized Streptomyces strains isolated from potato scab lesions in Finland and Sweden. A total of 1245 scab lesions was tested from potato cvs Matilda and Sabina grown in the field in two geographic regions of Finland in 2000 and 2001. Freshly harvested or stored potato tubers were incubated at room temperature (18–21°C) under humid conditions for a few days. Bacterial growth was collected from scab lesions for DNA isolation and PCR. The two scab pathogens were detected in the same potato fields, tubers and scab lesions. The relative incidence of S. scabies was high in freshly harvested tubers but was much lower than that of S. turgidiscabies following storage. Both pathogens were seed-transmitted in Matilda and Sabina after 24 weeks of storage at 4°C.