Angioinvasive lymphoma (lymphomatoid granulomatosis) in a cat,with cutaneous and ocular metastasis

Lymphomatoid granulomatosis (LYG) is a rare variant of an angioinvasive T-cell lymphoproliferative disorder that primarily affects the lungs, with common sites of metastasis including the skin and subcutis. In humans, it is a B-cell lymphoproliferative disorder associated with Epstein-Barr virus infection. Our case is a 7-y-old, spayed female, domestic longhair cat that decompensated and was euthanized following an initial diagnosis of angioinvasive lymphoma from a skin biopsy. Autopsy revealed nodules in the lungs and subcutis, and corneal thickening and cloudiness. Histologic examination of cutaneous nodules, lungs, and eye showed similar angioinvasive cellular infiltrates and pattern to that of the original skin biopsy, consistent with a diagnosis of LYG. The neoplastic cells displayed CD3-positive immunoreactivity in the skin, eye, and lung, and PCR for antigen receptor rearrangement (PARR) showed T-cell clonality in all tissues tested. This is the third case of LYG to be reported in cats and is the only case in which PARR analysis and immunophenotyping immunohistochemical staining was performed. LYG with ocular involvement has not been reported previously in cats, to our knowledge. Our case demonstrates the necessity for considering LYG when presented with a cat with respiratory signs in conjunction with subcutaneous nodules and ocular lesions.     

Draft Genome Sequencing of <Emphasis Type="Italic">Rhizoctonia solani</Emphasis> Anastomosis Group 3 (AG3- PT) Causing Stem Canker and Black Scurf of Potato

Rhizoctonia solani is a soil-borne basidiomycete fungus with a necrotrophic lifestyle being classified into fourteen reproductively incompatible anastomosis groups (AGs). AG3-PT (a potato subgroup) is associated with quantitative and qualitative yield losses through stem canker and black scurf in potato. Here we present the first draft sequence of the R. solani [AG3-PT] strain RS-20 with a G-C content of 48.3%. It consists of 11,431 total predicted protein coding regions including 181 tRNA and 31 rRNA coding genes. The initial pBLAST revealed more than 97% hits among AG groups where as only 1.7% of genes hit with other organisms. The R. solani genome is found to be dominated with tri mer repeats. The genome-wide evolutionary studies revealed the close association of AG3-PT with AG3. The draft sequence represents a helpful resource not only for understanding the core genes involved in pathogenecity but also evolution and adaptive behaviour within the R. solani species complex.     

Increasing water productivity with improved N fertilizer management

There is continuing debate about the role of water productivity and the potential to increase it in response to significantly increased water demand to meet the future needs for food—estimated to be roughly double that of today by 2050. The debate centers round the relative potential benefits of enhancing rainfed agriculture, improving irrigation and expanding areas of both. All expansion and intensification options will require significantly more water to be used, often in places where the ecosystem impacts of agriculture are already severe. Improvement in water productivity can result from improving the provision and management of the other factor inputs of crop production. There is considerable debate on the ability of other inputs—typically nitrogen—to substitute for water. This paper describes a set of simulations undertaken with well calibrated maize (Zea mays L.) crop model in Decision Support System for Agro-technology Transfer (DSSAT). The simulations investigate the response to nitrogen under rainfed conditions in Florida, and show that neither the transpiration ratio nor the harvest index are constant in practice, and that fertilizer use can enhance water productivity, even in quite high yield conditions and that the transpiration ratio can be increased by N fertilizer application at low levels of crop water use.