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21.
B. K. Dube Pratima Sinha Kanchan Shukla C. Chatterjee Vinay Kumar Pandey Anoop Dutt Rai 《Journal of plant nutrition》2013,36(6):996-1004
To examine tolerance of cadmium (Cd) by eggplant (Solanum melongena L.) cv. ‘Hybrid PK 123’, plants were grown in refined sand in complete nutrient solution for 52 days in a glasshouse at ambient temperature. Cadmium sulfate was superimposed on day 53, at variable levels: nil, 0.05, 0.1, 0.2, 0.4, and 0.5 mM. Influence of excess Cd was discernible after 5 days of metal supply at 0.4 and 0.5 mM Cd as depression in growth. At these levels, foliar symptoms were initiated as paling of young leaves at the base progressing upward. With increase in age, affected leaves turned golden yellow and these effects spread to lower leaves. Leaf size and floral initiation were very much restricted. These types of phenotypes induced leaf senescence. Excess Cd reduced the biomass and fruit yield of plants. At higher levels (>0.1 mM) of Cd, fruit formation was completely inhibited and fruits formed at 0.05 and 0.1 mM Cd were smaller in size. Besides this, excess Cd disturbed the metabolism of eggplant by reducing the concentration of chlorophyll (a and b), protein, Hill reaction activity, and activity of antioxidant enzymes—catalase and ascorbate peroxidase; whereas the activity of peroxidase and ribonuclease increased in leaves of eggplant. Cadmium excess reduced the concentration of Fe and Zn and Cd increased that of proline, lipid peroxidation, phenols, reducing sugars and Cd concentration in leaves of eggplant. 相似文献
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Patil K. Gouda Surapaneni Saikumar Chejerla M. K. Varma Kancharla Nagesh Sanka Thippeswamy Vinay Shenoy Mugalodim S. Ramesha Halagappa E. Shashidhar 《Plant Breeding》2013,132(1):61-69
Rice blast, caused by fungus Magnaporthe grisea, is a serious disease causing considerable economic damage worldwide. Best way to overcome disease is to breed for disease‐resistant cultivars/parental lines of hybrids. Pusa RH10, first aromatic, fine‐grain rice hybrid released and cultivated extensively in India. Hybrid and its parental lines, Pusa 6A and PRR78, are highly susceptible to blast. CO39 pyramid carrying two dominant, broad‐spectrum blast‐resistance genes, viz. Pi‐1 and Piz‐5, used as a donor parent to introgress these genes into PRR78 using marker‐assisted backcrossing (MABC). Microsatellite markers RM5926 and AP5659‐5 tightly linked to Pi‐1 and Piz‐5 genes, respectively, were used for foreground selection to derive introgression lines. Further, these lines were evaluated for agronomic performance, disease reaction and cooking quality traits along with PRR78. Most of the improved lines were on par with PRR78 for all traits evaluated except gelatinization temperature. Recurrent parent genome percentage (RPG) study also revealed similarity of these lines with PRR78. Hybrids derived using improved PRR78 lines were superior over Pusa RH10 in terms of yield. 相似文献
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Lalitha Devi Guvvala Pranitha Koradi Vinay Shenoy Lalitha Shanti Marella 《Journal of Crop Science and Biotechnology》2013,16(2):111-121
Mahsuri a popular traditional variety and the first rain-fed mega variety of the Indian sub- continent. It is highly susceptible to bacterial leaf blight caused by Xanthomonas oryzae pv.oryzae. Nine best performing families of Mahsuri pyramid containing four bacterial blight resistance genes (Xa4, xa5, xa13 and Xa21) were evaluated for agronomic, yield and its related characters viz. days to 50% flowering, plant height, number of tillers, panicle length, filled grains per panicle, grain weight, and yield under natural and disease pressure conditions for three consecutive wet seasons. In addition these pyramids were also evaluated for three different spacings to find out the optimum spacing under disease free and disease pressure conditions. Results revealed that under disease free conditions there was no significant difference between the pyramids and the parent for the characters evaluated in each spacing. However characters plant height, number of tillers, panicle length, filled grains per panicle, yield per plant and yield per sq. m. showed significant variation between the different spacings across seasons. Under heavy disease pressure the parent exhibited highly susceptible reaction whereas the pyramid families were highly resistant. A wider spacing had less yield loss when compared to dense planting under BB infestation in case of parent. There was no such yield loss in the pyramid families. When yield per sq. m. was taken into consideration the 20 × 20 cm spacing showed the highest yield when compared to the other two spacings since number of plants were more. The pyramids insulated the yield loss against bacterial leaf blight and are a gain to the farmers to help overcome the heavy yield losses due to this disease. These pyramids have the potential to replace the parent and can be used directly. In addition they can be used as donors for bacterial blight resistance in any breeding program. 相似文献
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Arjunan Jeevalatha Swarup Kumar Chakrabarti Sanjeev Sharma Vinay Sagar Kamlesh Malik Baswaraj Raigond Bir Pal Singh 《Phytoparasitica》2017,45(1):33-43
The complete coat protein (cp) gene sequence of eighty Tomato leaf curl New Delhi virus-[potato] (ToLCNDV-[potato]) isolates collected from eleven states were determined. Phylogenetic analysis based on cp gene grouped the isolates into two major clades (I & II) and they shared 95.9–100.0% identity. The DNA A and DNA B of eight representative isolates (six from clade I and two from clade II) were 2739–2740 and 2692–2694 nts long and shared 94.6–99.4% and 97.2–99.5% homology within the isolates, respectively. Among the eight isolates, the DNA A of two isolates (Clade II), GWA-5 and FAI-19 had 94.6–95.3% sequence identity to other six isolates and formed a sub-clade within the ToLCNDV-[potato] isolates. Similar grouping was also revealed with AC1 and AC4 genes of these eight isolates. The DNA A components shared more than 90.0% identity with the DNA A of ToLCNDV isolates from cucurbitaceous crops, tomato, bhendi, 89.0–90.0% with ToLCNDV-papaya isolates and 70.4–74.0% with other tomato leaf curl viruses. Hence, the begomovirus infecting potatoes are the ToLCNDV isolates, designated as ToLCNDV-[potato]. Whereas, the DNA B components shared 86.6–91.7% identity with ToLCNDV isolates from cucurbits, tomato and bhendi. Evidence for intra-species recombination was detected only in DNA A with a maximum of three events in GWA-5 and FAI-19 isolates. Analysis of cp gene, DNA A, iterons and recombination events clearly indicate that two groups of ToLCNDV-[potato] infects potato in India. 相似文献
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A commercially developed cytokinin and seaweed extract formulation “Dravya” was used at 0.3% concentration for soaking of sorghum seeds for 12 h to test its effect on germination, vigour index, chlorophyll content and defense enzyme activity against seed mycoflora of sorghum. Among the treatments, Dravya (0.3%) with Dithane M-45 (0.1%) and GLSTIN (0.1%) or separately or in combination resulted is maximum percentage of seed germination, seedling vigour and reduced the incidence of seed mycoflora besides defense enzymes enhancement was noticed. In this study, Dravya was used for seed soaking, foliar spray, separately as well as in combination with GLSTIN and Mancozeb M-45 to test its efficacy in the enhancement of seedling vigour, chlorophyll content and defence enzyme activities. Hence, it is inferred that Dravya is a good growth promoter and improves natural resistance to disease in sorghum. 相似文献
26.
北方旱区保护性耕作对农田土壤水分的影响 总被引:22,自引:10,他引:12
土壤水分是中国北方旱区农业生产的主要限制因子,研究保护性耕作技术体系下土壤水分的动态变化,明确不同耕作模式下的水分平衡规律,对于选择适宜的保护性耕作技术,提高北方旱区土壤水分的利用效率具有重要的指导意义。该文在2a田间试验的基础上采用DSSAT模型对4个不同保护性耕作处理的土壤体积含水量、水分平衡以及水分利用效率进行了模拟和检验。结果表明干旱年份保护性耕作处理土壤体积含水量较传统耕作高,RMSE误差在0.025~0.063;干旱年份传统耕作土壤储水量减少最多,为144.6mm,降水较多年份减少也最多,为46.1mm;干旱年份水分利用效率1.52~1.78kg/m3,免耕覆盖水分利用效率最高,降水较多年份水分利用效率1.70~1.71kg/m3,各处理间差异并不显著。研究结果为保护性耕作技术对农田土壤水分的影响研究提供了理论依据。 相似文献
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Paprotka T Delviks-Frankenberry KA Cingöz O Martinez A Kung HJ Tepper CG Hu WS Fivash MJ Coffin JM Pathak VK 《Science (New York, N.Y.)》2011,333(6038):97-101
The retrovirus XMRV (xenotropic murine leukemia virus-related virus) has been detected in human prostate tumors and in blood samples from patients with chronic fatigue syndrome, but these findings have not been replicated. We hypothesized that an understanding of when and how XMRV first arose might help explain the discrepant results. We studied human prostate cancer cell lines CWR22Rv1 and CWR-R1, which produce XMRV virtually identical to the viruses recently found in patient samples, as well as their progenitor human prostate tumor xenograft (CWR22) that had been passaged in mice. We detected XMRV infection in the two cell lines and in the later passage xenografts, but not in the early passages. In particular, we found that the host mice contained two proviruses, PreXMRV-1 and PreXMRV-2, which share 99.92% identity with XMRV over >3.2-kilobase stretches of their genomes. We conclude that XMRV was not present in the original CWR22 tumor but was generated by recombination of two proviruses during tumor passaging in mice. The probability that an identical recombinant was generated independently is negligible (~10(-12)); our results suggest that the association of XMRV with human disease is due to contamination of human samples with virus originating from this recombination event. 相似文献
29.
S. K. Chakrabarti B. P. Singh Garima Thakur Jagesh K. Tiwari S. K. Kaushik Sanjeev Sharma Vinay Bhardwaj 《Potato Research》2014,57(1):1-11
Several quantitative trait loci (QTL) for resistance to late blight have been reported in diploid potatoes. The diploid wild potato species Solanum chacoense possesses a high degree of horizontal resistance to late blight. In the present study, we report on QTL mapping for late blight resistance in a diploid mapping population of 126 F1 of Solanum spegazzinii (susceptible) × S. chacoense (resistant). The area under the disease progress curve (AUDPC) values for late blight resistance using the “whole plant in vitro assay” and the “detached leaf assay” on the mapping population displayed quantitative variation. Out of 64 AFLP primer-pairs combinations and SSR markers, a total of 209 significant AFLP loci were placed onto the 12 linkage group of potato covering a total map length of 6548.1 cM. QTL analysis based on the AUDPC dataset of the “whole plant in vitro assay” using the interval-mapping option identified two QTL (LOD?>?2.5) located on linkage groups IX and X, which explained 14.70 and 3.40% variation, respectively. The present study revealed the presence of potential new genetic loci in the diploid potato family contributing to quantitative resistance against late blight. 相似文献
30.
Among the non-timber forest products, the medicinal and aromatic plants (MAP) are key forest resources for the world’s herbal medicine, cosmetics, health food and other natural products industries. MAP businesses are continuing to increase in many biodiversity-rich areas of the world. The overharvesting and depletion of forest based MAP and their unregulated trade are creating ecosystem and business sustainability issues for the MAP industry. This paper assesses the current state of the MAP industry in Uttarakhand State in the northwestern Himalayan region of India and offers marketing strategies for the conservation and sustainable commercialization of MAP in this region. A qualitative research method was first used involving interviews with policymakers and other industry stakeholders including local MAP traders and growers. A strengths, weaknesses, opportunities and threats analysis and a quantitative strategic planning matrix analysis were then conducted to identify appropriate marketing strategies. The study reveals that the existing practices of MAP businesses in Uttarakhand are inconsistent with the plants’ conservation requirements and the livelihoods of the primary producers. In its current form, the region’s MAP industry is therefore in a precarious position. However, if policymakers consider and act on the industry’s strengths and opportunities they could positively influence its sustainable development and help to minimize the negative impacts of the trade on the region’s forest MAP resources. 相似文献