Possible sex differences in the susceptibility of calves to rotavirus infection.

The agar gel precipitation test was used to detect rotavirus antibodies in the serum of 562 calves and bovine rotavirus antigen in the feces of 347 calves. Significantly more females had rotavirus antibodies in the serum (P less than 0.01) and rotavirus antigen in the feces (P less than 0.1) than did male calves. Female buffalo calves were also found to be more susceptible than male buffalo calves to rotavirus infection.     

Detection of goat pox antigen and antibody by the counter immunoelectrophoresis test

Summary The counterimmunoelectrophoresis (CIE) test was standardised for the detection of goat pox antigen and antibody using inactivated antigens. The chloroform inactivated and live antigens were equally sensitive for detection of goat pox precipitins. The precipitinogens of goat pox virus (GPV) were found to be soluble in nature. The CIE test was quick as well as more sensitive than the agar gel precipitation test for detection of GPV antibody/antigen. The CIE employing inactivated antigen has been used for the first time in the detection of GPV antibodies/antigens.

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Deteccion Del Antigeno Y Anticuerpos De Viruela Caprina Mediate La Prueba De Contrainmunoelectroforesis

Resumen Se estandarizó la prueba de contrainmunoelectroforesis, para la detección del antígeno y anticuerpos del virus de la viruela caprina, usando antígenos inactivados. El antígeno inactivado con cloroformo y el antígeno vivo, fueron igualmente sensitivos para la detección de precipitinas de viruela caprina. Los precipitógenos del virus de la viruela caprina se encontraron que eran solubles. La prueba de contrainmunoelectroforesis fue más rápida y más sensitiva que la precipitación agar gelatina para la detección de anticuerpos/antígenos del virus de la viruela caprina. La prueba de contrainmunoelectroforesis con antígeno inactivado ha sido utilizada por vez primera en la detección de anticuerpos/antígenos del virus de la viruela caprina.

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Detection De l'Antigene Et De l'Anticorps Variole Caprine Par Un Test De Contrimmuno-Electrophorese

Résumé Le test de contrimmuno-électrophorèse (CIE) a été standardisé pour la détection de l'antigène et de l'anticorps variole caprine avec des antigènes inactivés. Les antigènes vivants et inactivés par le chloroforme sont de sensibilité équivalente pour la détection des précipitines variole caprine. On a montré que ces précipitogènes du virus variole caprine (VVC) étaient de nature soluble. Le CIE est rapide et plus sensible que le test de précipitation en gélose pour la détection des antigènes et anticorps VVC. C'est la première fois que le CIE mettant en oeuvre un antigène inactivé a été utilisé.

     

Alternate Selection Indices for Leaf Photosynthetic Rate in Greengram (Vigna radiata [L.] Wilczek) Cultivars

Significant differences were observed for leaf net photosynthetic rate, chlorophyll content, soluble protein content, nitrate reductase activity, stomatal frequency and specific leaf mass among ten greengram cultivars. Leaf net photosynthetic rate had significant positive correlation with soluble protein, nitrate reductase activity, stomatal frequency and specific leaf mass. Leaf net photosynthetic rate had no relationship with leaf chlorophyll content in greengram.     

An Analysis of Grain Development in Oryza sativa L. Cultivars

A field study was conducted to determine if differences in grain filling rate and duration existed in two early cultivars of rice. There is no cultivar difference in duration of grain filling and grain matured in about 30 days from anthesis in both the cultivars. The effective grain filling period in rice is only 10 days between 3rd and 13th day from anthesis. There is significant difference in grain filling rate and is positively related to grain weight. Grain filling rate is more important than duration of filling for achieving higher grain size and thus yield in rice.     

Functional Growth Model for Pod Development in Greengram and Blackgram

Identification of readily measured traits that are associated with higher productivity of field crops is important to breeders. The objective of this study was to find out whether pod filling rate or duration of pod filling greatly decide final pod weight in greengram and blackgram. The effective pod filling duration is only 16 days in both the crops. Pod filling rate is more important than duration of pod development. Richards function is found to more closely fit the data on pod development from anthesis to maturity in both the species.     

Oestrus synchronisation and fertility in black Bengal goats following administration of progesterone/prostaglandin and gonadotrophins.

Oestrus synchronisation, fertility and kidding behaviour were studied in 44 Black Bengal goats. They were divided into six experimental groups: group 1, control; group 2, progesterone; group 3, progesterone, pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG); group 4, prostaglandin F2 alpha (PGF2 alpha); group 5, PGF2 alpha, PMSG and HCG; group 6, PMSG and HCG. There was 100 per cent synchronisation of oestrus in the groups treated with progesterone, progesterone with PMSG and HCG, and prostaglandin with PMSG and HCG. In the other two treated groups the synchronisation was between 66 and 75 per cent. In the control group only 50 per cent of the animals came into oestrus during the period of observation. The duration of oestrus varied between 19 and 24 hours except in group 5 where it was 40.87 hours. Animals came on heat between 95 and 137 hours after treatment except in group 5 where the interval was only 18.87 hours. A maximum fertility of 75 per cent was observed in group 4 while the kidding percentage was greatest in group 2. There appeared to be no beneficial effect of superovulation on the number of kids produced. Gestation length was similar in all the groups.     

Fatal intraoperative pulmonary fat embolism during cemented total hip arthroplasty in a dog

A 3-year-old, German shepherd dog died suddenly during cemented total hip arthroplasty. Gross necropsy findings included severe pulmonary edema and congestion as well as congestion of the liver and kidneys. Acute pulmonary embolism was suspected as the cause of death. Microscopic examination of hematoxylin and eosin-stained, formalin-fixed, and oil red O-stained frozen tissue sections confirmed the presence of large numbers of fat globules in blood vessels in the lungs, liver, and kidneys. Fat embolism during total hip arthroplasty is a common surgical complication in humans, but it is uncommon in veterinary cases and is rarely a cause of death.     

Regulation of B cell tolerance by the lupus susceptibility gene Ly108

The susceptibility locus for the autoimmune disease lupus on murine chromosome 1, Sle1z/Sle1bz, and the orthologous human locus are associated with production of autoantibody to chromatin. We report that the presence of Sle1z/Sle1bz impairs B cell anergy, receptor revision, and deletion. Members of the SLAM costimulatory molecule family constitute prime candidates for Sle1bz, among which the Ly108.1 isoform of the Ly108 gene was most highly expressed in immature B cells from lupus-prone B6.Sle1z mice. The normal Ly108.2 allele, but not the lupus-associated Ly108.1 allele, was found to sensitize immature B cells to deletion and RAG reexpression. As a potential regulator of tolerance checkpoints, Ly108 may censor self-reactive B cells, hence safeguarding against autoimmunity.