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81.
Juan Luis H. Cardós Isabel Martínez Victoria Calvo Gregorio Aragón 《Landscape Ecology》2016,31(9):1975-1995
Context
Mediterranean forests have been fragmented intensively over time, thereby yielding small and isolated forest remnants. They host a rich variety of epiphytes, which may be affected by landscape structure. Previous studies have analyzed the influence of habitat quality on these epiphytic communities, but there is little knowledge of the effects of other fragment features.Objectives
We evaluated the impacts of forest loss and fragmentation on epiphytic communities (lichens and bryophytes) at plot and fragment scales after controlling the variation in forest structure and management.Methods
We considered 40 fragments of dense oak forests in a human-modified landscape. We quantified their spatial attributes (size and shape), the quality of the surrounding matrix and the forest stand structure. We modeled community traits, and the presence and abundance of species at fragment and plot scales.Results
Fragment size, shape, and the quality of the surrounding matrix were key factors that affected epiphytic richness and diversity. Larger and more regularly shaped fragments hosted the richest and most diverse communities, possibly offering a larger core area and thus favoring the entry of typical forest species. A high-contrast matrix was only favorable in small fragments, probably allowing the arrival of propagules. The species-level response was highly variable.Conclusions
Landscape structure provides powerful explanations of the richness and diversity losses among epiphytes. Forest management should ensure the retention of the largest possible continuous forests. The management strategy of the matrix will depend on the conservation goal, since we observed different effects related with quality and fragment size.82.
Alessandra Torina Victoria Naranjo Maria Grazia Pennisi Tatiana Patania Fabrizio Vitale Pietro Laricchiuta Angelina Alongi Salvatore Scimeca Katherine M Kocan José de la Fuente 《Journal of zoo and wildlife medicine》2007,38(4):591-593
Lions (Panthera leo) are an endangered species threatened by illegal hunting, habitat loss, and infectious diseases. Little is known about the tick-borne pathogens that infect lions and could contribute to population declines. The objective of this study was to characterize Rickettsia spp., Anaplasma phagocytophilum, and Coxiella burnetii infections in 10 lions from the Fasano Safari Park in Italy by serology, polymerase chain reaction, and sequence analysis. Although animals did not show clinical signs of tick-borne diseases, evidence of infection with C. burnetii, spotted fever group Rickettsia sp., and A. phagocytophilum were found in 50%, 20%, and 10% of the lions, respectively. One of the lions tested positive for all three pathogens. This study is the first report of molecular evidence of infection with C. burnetii, Rickettsia sp., and A. phagocytophilum in lions and provides evidence that these felids become infected and serve as hosts for tick-transmitted bacteria. 相似文献
83.
Victoria González-Rodríguez Rafael Villar Raquel Casado Elena Suárez-Bonnet José Luis Quero Rafael M. Navarro-Cerrillo 《Annals of Forest Science》2011,68(7):1217-1232
Introduction
The great spatial and temporal heterogeneity of Mediterranean ecosystems can influence establishment success in woody species, whose natural regeneration occurs to a very small extent. In this work, the effect of the spatial pattern of environmental variables (light availability, soil moisture and herbaceous production) on seedling emergence, growth, survival and establishment success was examined by using a spatially explicit approach. 相似文献84.
Evaluation of a serum-based PCR assay for the diagnosis of canine monocytic ehrlichiosis 总被引:1,自引:0,他引:1
Mathios E. Mylonakis Victoria I. Siarkou Leonidas Leontides Eleftheria Bourtzi-Hatzopoulou Vassilios I. Kontos Alexander F. Koutinas 《Veterinary microbiology》2009,138(3-4):390-393
The aim of this study was to estimate the relative diagnostic sensitivity and specificity of a polymerase chain reaction (PCR) assay in the serum of dogs with naturally occurring non-myelosuppressive canine monocytic ehrlichiosis (CME), and to investigate the association between PCR positivity and immunofluorescence antibody (IFA) titres for Ehrlichia canis. Serum samples obtained from 38 dogs with non-myelosuppressive CME and 12 healthy dogs were analyzed retrospectively. Each serum sample was analyzed in triplicate using an E. canis-specific nested PCR assay targeting a 389 bp sequence of the 16S rRNA gene. E. canis DNA was amplified in 24 of 38 (63.1%) affected dogs; all samples from healthy dogs were negative. A high level of agreement was found among the PCR replicates (P < 0.0001). Median IFA titre of the 24 PCR-positive dogs was significantly lower than that of the PCR-negative infected dogs (P = 0.0029), indicating that E. canis DNA may circulate prior to the development of a high antibody titre. Serum-based PCR analysis is suggested for the early diagnosis of CME when whole blood samples are not available. 相似文献
85.
86.
Enterocolitis caused by Yersinia pseudotuberculosis was identified by bacterial culture and histopathology in a 5-month old suckler calf. No other significant enteric pathogens were detected. Diagnosis of this condition can be missed if selective media are not used for bacteriology and if histopathology is not attempted or the alimentary tract is unsuitable for examination. This is the first case report of Y. pseudotuberculosis infection in a calf in the United Kingdom. 相似文献
87.
Spontaneous hemoperitoneum and anaphylactic shock associated with Hymenoptera envenomation in a dog 下载免费PDF全文
88.
89.
Moreno-Labanda JF Mallavia R Pérez-Fons L Lizama V Saura D Micol V 《Journal of agricultural and food chemistry》2004,52(17):5396-5403
The presence of stilbenes in wine is becoming an important issue due to their claimed relation to a low incidence in coronary diseases and their increasing implication as cancer chemopreventive and neuroprotective agents. Total resveratrol content, quantified as glucoside and aglycone forms of resveratrol, has been determined in a survey of 45 Monastrell monovarietal Spanish red wine types (around 135 wine samples), belonging to Alicante and Bullas appellations. The average between ratio glucoside/aglycone forms of resveratrol in these wines was considerably high, ranging from 82 to 91% of resveratrol in its glycosidic form. This characteristic was observed in a high percentage of the studied wines, which were made under different winemaking procedures, and from different vintages (1995-2002). In addition, wines made using macerative fermentations with double amount of solid parts ("doble pasta") reached the highest levels of total stilbene content expressed as resveratrol equivalent, i.e., 30 mg/L (average of 18.8 mg/L). It can be concluded that high resveratrol glucoside concentration and low free isomer content can be considered characteristics of the Monastrell variety, as it happens to red wines deriving from other varieties grown at warm climates. This fact, also observed for other French and Portuguese red varieties, might play an important role in food habits involving these types of wines. 相似文献
90.
Josefina Racedo María F. Perera Romina Bertani Claudia Funes Victoria González María I. Cuenya Angélique D′Hont Björn Welin Atilio P. Castagnaro 《Euphytica》2013,191(3):429-436
Brown rust, caused by the fungus Puccinia melanocephala, is responsible for important yield losses in sugarcane production globally and it is therefore an important objective to introduce resistance to this disease in breeding programs. A major gene, Bru1, has been shown to confer resistance to P. melanocephala strains from different parts of the world and two molecular markers, R12H16 and 9O20-F4, closely associated to this gene have been previously reported. The usefulness of these molecular diagnostic markers in order to predict a rust resistant phenotype under natural high pressure inoculums conditions was analyzed. A total of 129 sugarcane accessions were evaluated under field infection for resistance or susceptibility to brown rust and subsequently screened for presence or absence of the two Bru1 diagnostic markers. A total of 49 genotypes (38 %) were phenotyped as resistant to brown rust but only eight (16.3 %) of them were harboring the Bru1 gene. To determine overall frequency of the Bru1 in the local sugarcane germplasm collection, 190 additional genotypes were examined. Presence of Bru1, as determined by the diagnostic markers, was detected in only 7 % of the genotypes evaluated. In conclusion, Bru1 diagnostic markers enable positive selection for brown rust resistance in sugarcane and moreover allowed detecting at least one additional source(s) of resistance. Interestingly, whilst only little genetic variability of rust resistance independent of Bru1 has been reported previously, this alternative genetic resource(s) found in our local germplasm constitutes the predominant one and should be helpful in order to amplify the narrow genetic basis for brown rust resistance in sugarcane. 相似文献