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31.
Merhej V Cammilleri S Piquet P Casalta JP Raoult D 《Comparative immunology, microbiology and infectious diseases》2012,35(1):45-49
Coxiella burnetii, the causative agent of Q fever, may cause culture-negative vascular graft infections that can be diagnosed by serology and molecular biology. We present a case of vascular graft infection detected by positron emission tomography (PET) scanner. The presence of C. burnetii was confirmed by high antibody titers and positive polymerase chain reaction specific for C. burnetii. This report emphasizes the relevance of the PET scanner in the diagnosis of infection when used in association with Q fever serology and molecular biology for the etiological identification of C. burnetii. 相似文献
32.
William W.L. Cheung Vicky W.Y. Lam Jorge L. Sarmiento Kelly Kearney Reg Watson Daniel Pauly 《Fish and Fisheries》2009,10(3):235-251
Climate change can impact the pattern of marine biodiversity through changes in species’ distributions. However, global studies on climate change impacts on ocean biodiversity have not been performed so far. Our paper aims to investigate the global patterns of such impacts by projecting the distributional ranges of a sample of 1066 exploited marine fish and invertebrates for 2050 using a newly developed dynamic bioclimate envelope model. Our projections show that climate change may lead to numerous local extinction in the sub‐polar regions, the tropics and semi‐enclosed seas. Simultaneously, species invasion is projected to be most intense in the Arctic and the Southern Ocean. Together, they result in dramatic species turnovers of over 60% of the present biodiversity, implying ecological disturbances that potentially disrupt ecosystem services. Our projections can be viewed as a set of hypothesis for future analytical and empirical studies. 相似文献
33.
Asadhawut Hiranrat Darren C. Holland Wilawan Mahabusarakam John N. A. Hooper Vicky M. Avery Anthony R. Carroll 《Marine drugs》2021,19(2)
Two new fluorescent pteridine alkaloids, tedaniophorbasins A (1) and B (2), together with the known alkaloid N-methyltryptamine, were isolated, through application of mass directed purification, from the sponge Tedaniophorbas ceratosis collected from northern New South Wales, Australia. The structures of tedaniophorbasins A and B were deduced from the analysis of 1D/2D NMR and MS data and through application of 13C NMR DFT calculations. Tedaniophorbasin A possesses a novel 2-imino-1,3-dimethyl-2,3,7,8-tetrahydro-1H-[1,4]thiazino[3,2-g]pteridin-4(6H)-one skeleton, while tedaniophorbasin B is its 2-oxo derivative. The compounds show significant Stokes shifts (~14,000 cm−1) between excitation and emission wavelengths in their fluorescence spectra. The new compounds were tested for bioactivity against chloroquine-sensitive and chloroquine-resistant strains of the malaria parasite Plasmodium falciparum, breast and pancreatic cancer cell lines, and the protozoan parasite Trypanosoma brucei brucei but were inactive against all targets at 40 µM. 相似文献
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Davis MH Ponnamperuma GG McAleer S Dale VH 《Journal of veterinary medical education》2006,33(4):578-587
The Objective Structured Clinical Examination (OSCE) has become an excellent tool to evaluate many elements of a student's clinical skills, especially including communication with the patient (human medicine) or client (veterinary medicine); eliciting clinical information from these conversations; some aspects of the physical examination; and many areas of clinical evaluation and assessment. One key factor is that the examination can be structured to compare different students' abilities. 相似文献
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Melinda J. Mayer Joanna Steel D. Vicky Child John A. Hargreaves John A. Bailey 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(9):815-827
Infection of young roots of maize (Zea mays L.) by the parasitic plant Striga hermonthica (Del.) Benth. was examined. Attachment to and penetration of roots occurred within 1–2 days after inoculation. Subsequent growth through the cortex to the host stele and proliferation of parasite xylem tissue was commonly completed by 3–4 days after inoculation. Histochemical staining showed that young maize roots do not contain major wall-thickening components. However, an increase in cell wall fluorescence and endodermal cell wall thickness was often seen at the site of infection and in the surrounding maize root tissue at 3 days after inoculation. This host response was variable and did not prevent rapid and successful penetration by the parasite. In contrast, uninfected roots of Pennisetum setosum (Sw.) L. Rich., a species resistant to S. hermonthica, had substantial thickening of the inner endodermal cell walls and exhibited further cell wall thickening at the stele upon infection. Examination of infections on both hosts demonstrated the presence of autofluorescent material at the host-parasite interface. This material was thicker and more extensive at the P. setosum-S. hermonthica interface than at the maize-S. hermonthica interface, and contained polyphenols and lignin. Examination of the host-parasite xylem connections in maize revealed substantial invasion of the host stele by both parenchyma and tracheary elements. In a few cases of P. setosum infection, parasite cells entered the stele; however, this did not lead to successful establishment of the parasite. 相似文献
39.
T-helper cells from naive to committed 总被引:3,自引:0,他引:3
An endotoxin-induced mastitis model was used to study permeability changes associated with increased milk matrix metalloproteinase (MMP) activity in early inflammation. One quarter of two cows was inoculated with endotoxin (Escherichia coli 055:B5). Blood, milk, and whey were collected before and repeatedly after inoculation for 48 h. The profile and amounts of gelatinolytic MMPs were determined by zymography; gelatinase A (72 kD MMP-2) and gelatinase B (92 kD MMP-9) were identified by Western immunoblotting. Bovine serum albumin (BSA) and trypsin inhibitor capacity (TIC) were used as markers of capillary permeability with parallel examination of neutrophil penetration from blood to milk. Five clinical E. coli mastitis milk samples and five milk samples from cows with healthy udders were analyzed to detect whether increased levels of gelatinolytic MMP-2 and MMP-9 have a role in naturally occurring mastitis with endotoxin involvement. Milk MMP levels increased 2h after the endotoxin challenge. Both MMP-2 and MMP-9 were involved in this early proteolytic event. These increased MMP levels are associated with increased capillary permeability, evidenced first by the penetration of small molecular weight proteins, such as BSA and TIC. Later, 6-12h post endotoxin inoculation, neutrophilic leucocytes also entered the site, as they require larger tissue damage in basal membrane and interstitial tissue structures than BSA and TIC to extravasate. In naturally occurring disease, increased MMP-2 and MMP-9 levels were detected in milk. Thus, gelatinases, especially MMP-2, are involved in the early degradation of the blood-milk barrier, which precedes the penetration of blood-derived cellular components into milk in endotoxin-induced mastitis. In the future, measuring MMP in milk/whey might be a useful tool for diagnosing early mastitis. 相似文献
40.
Heri Ann Limley Graham Bruce Crosbie Kay Kong Lim Hannah Grace Williams Dean Aris Diepeveen Vicky Ann Solah 《Cereal Chemistry》2013,90(3):231-239
Australia exports 75% of the wheat produced annually, and the Asian region is an important export market for Australian wheat. These markets require suitable quality grain for use in wheat‐based products, so the understanding of wheat end products from an Asian consumer point of view is important. The major wheat quality requirements for char siew bao, a Chinese Guangdong‐style steamed bun, were determined. The research 1) developed laboratory methods for the production and assessment of char siew bao using a scoring system; 2) optimized the new methods and compared the new methods with a traditional method; and 3) used the new validated methods in an investigation of the role of soft wheat quality on char siew bao. A sponge‐and‐dough method with 1 hr fermentation time was developed to establish a simplified laboratory method for char siew bao production. The screening experiments and response surface methodology experiments showed that water addition, sugar, and sponge mixing time were responsible for 69% of the variation in total score. Dough strength was the most important parameter affecting char siew bao quality, followed by flour swelling volume, protein content, and dough extensibility. Both protein and starch quality were important determinants of char siew bao quality. 相似文献