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81.
A Arenas S Vicente I Luque J C Gomez-Villamandos R Astorga A Maldonado C Tarradas 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1999,46(6):399-404
We have carried out an aetiological and pathological study of an outbreak of septicaemia caused by the O165 serogroup of Escherichia coli in Japanese quail (Coturnix coturnix japonica) which resulted in high mortality (90%). After treatment with amoxicilin in drinking water (200 mg/l), morbility and mortality rates dropped markedly. Microbiological studies showed that the organism responsible was an atypical E. coli strain, on the basis of the non-fermentation of lactose (ONPG-), which belonged to the O165 serogroup and was highly virulent for 1-day-old chickens. 相似文献
82.
83.
Kelly Lana Araújo Silvaldo Felipe da Silveira Vicente Mussi-Dias Juan Manuel Anda Rocabado Flavio Costa Miguens 《European journal of plant pathology / European Foundation for Plant Pathology》2017,148(2):413-422
Two Camarotella (Phyllachoraceae) species, C. torrendiella and C. acrocomiae are the causative agents of small (SV) and large verrucosis (LV), respectively, which are important diseases affecting Brazilian coconut palms. The small verrucosis produces necrotic lesions in coconut palm leaflets, whereas LV just produces chlorosis. Semi-thin sections of asymptomatic leaflets and of leaves presenting stromata in different development stages were compared through light microscopy in order to characterize the colonization process of these two coconut palm verrucosis agents. Camarotella torrendiella initially colonized the adaxial epidermal cells and the cells underlying the epidermis close to the vascular bundles. In latter colonization stages, the hyphae of C. torrendiella remained limited to the underlying necrotic tissue adjacent to the mature stromata, mostly in the intracellular spaces and in the collapsing cells of necrotized tissues around the vascular bundles. This species does not colonize intracellular intact fiber cells, xylem vessels or phloem sieve tube elements. In contrast, C. acrocomiae presented a typical biotrophic parasitism model such as that of some gramineous Phyllachora spp. High densities of C. acrocomiae hyphae were found inside intact sieve tube elements; however, with no evidence of cellular death. The extensive hyphal colonization by C. acrocomiae within sieve tube elements was also observed in tertiary and quaternary bundles, as well as in anastomosing vascular bundles. The dependence of both species on the colonization sites associated with vascular bundles indicates the need for additional studies about these intricate host-pathogen relationships. These studies could be important to define new strategies to control coconut palm verrucosis diseases. 相似文献
84.
85.
Allepuz A Casal J Napp S Saez M Alba A Vilar M Domingo M González MA Duran-Ferrer M Vicente J Alvarez J Muñoz M Saez JL 《Preventive veterinary medicine》2011,100(1):441-52
In this study we explored the spatial variation of Bovine tuberculosis (BTB) risk of being positive, new positive or persistently positive, as well as the risk of eliminating BTB in positive herds throughout Spain from 2006 to 2009 by means of hierarchical Bayesian models. The results of the models showed that the risk of infection (positive or new positive herds), persistence and elimination was lower in counties located in north and north-eastern of Spain, and in the Balearic and Canary islands than in the rest of the country. In some counties the risk of positivity was high during the four years of study, whereas there were others where the risk of positivity was high only in some of the years. With regard to the risk of persistence of BTB positive herds, counties located in the central, western and south-western part of the country had a higher risk in the three studied periods. This study has identified some specific areas of increased BTB risk in Spain, information that is useful for disease management. 相似文献
86.
Vicente Rozas José Miguel Olano Lucía DeSoto David Bartolomé 《Annals of Forest Science》2008,65(8):809-809
87.
Rocío Santiago Roberto de Armas Blanca Fontaniella Carlos Vicente María-Estrella Legaz 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(3):439-450
The accumulation of soluble and cell wall-bound phenolics in the sugarcane stems of young plants from highly resistant cv.
My 5514 and susceptible cv. B 42231, inoculated or not inoculated with smut sporidia, was studied. The ratio of inoculated
to uninoculated plants of some cell wall-bound phenolics, such as ferulic, caffeic, and syringic acids increased for the resistant
cv. My 5514, whereas it was maintained more or less constantly for the susceptible cv. B 42231. The highest increase of this
ratio in the resistant cv. My 5514 corresponded to both caffeic and syringic acids. This could result in a better capacity
to cv. My 5514 for an increase in the frequency of bridges between lignin fragments through ester-ether linkages for reinforcing
the cell wall and major resistance to the disease. This reinforcement of the cell wall could provide an effective barrier
to pathogen entry and spread. Soluble sub-fractions of all phenolics detected showed non-stable patterns. Caffeic acid, that
regulates phenylalanine ammonia-lyase activity in sugarcane, showed a significant decrease in its titre at 24 h in the resistant
cultivar, principally in the free soluble fraction, whilst the susceptible cultivar enhanced it. We hypothesise that the pathway
of hydroxybenzoic acids is only activated once the level of p-coumaric acid justifies the accumulation of hydroxycinnamic acids required for reinforcing the cell wall after inoculation. 相似文献
88.
Frederic Aparicio Salvador Soler José Aramburu Luis Galipienso Fernando Nuez Vicente Pallás Carmelo López 《European journal of plant pathology / European Foundation for Plant Pathology》2009,123(1):117-123
A polyprobe for the simultaneous detection by non-isotopic molecular hybridisation has been developed to detect any of the
following six viruses causing important economic losses in tomato crops: Tomato spotted wilt virus, Tomato mosaic virus, Pepino mosaic virus, Cucumber mosaic virus, Potato Y virus and Parietaria mottle virus. The polyprobe detected all six viruses with similar sensitivity to that obtained using individual riboprobes. In addition,
we evaluated the possible use of the tissue-printing as a sample preparation technique applied to routine diagnosis of tomato
plants with the polyprobe.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
89.
Evaluation of biological control agents for managing cucurbit powdery mildew on greenhouse-grown melon 总被引:1,自引:0,他引:1
D. Romero A. de Vicente H. Zeriouh F. M. Cazorla D. Fernández-Ortuño J. A. Torés A. Pérez-García 《Plant pathology》2007,56(6):976-986
An evaluation was made of the ability of two mycoparasite-based products AQ10® ( Ampelomyces quisqualis ) and Mycotal® ( Lecanicillium lecanii ), as well as three strains of Bacillus subtilis , to manage powdery mildew disease, caused by Podosphaera fusca on melon seedlings maintained under different regimes of relative humidity and on plants grown under greenhouse conditions in Spain. In every case fungal and bacterial biocontrol agents (BCAs) performed better under conditions of high relative humidity (90–95% RH). In greenhouse experiments, the effectiveness of the mycoparasites to manage powdery mildew was absolutely dependent on mineral oil. The strains of B. subtilis provided disease control similar to that achieved with the mycoparasites or the fungicide azoxystrobin. Microscopic analysis showed the ability of these bacterial strains to efficiently colonize leaf surfaces and revealed the occurrence of antagonistic interactions between biological agents and P. fusca structures. These results confirmed the usefulness of these BCAs for managing powdery mildew on greenhouse-grown cucurbits either as single products or as a component of integrated control programmes. 相似文献
90.
Characterization,genetic diversity and distribution of Xanthomonas campestris pv. campestris races causing black rot disease in cruciferous crops of India 下载免费PDF全文
Black rot, caused by Xanthomonas campestris pv. campestris (Xcc) is a disease of crucifer crops. The objective of this study was to characterize races of Xcc, their distribution and genetic diversity in India. Two hundred and seventeen isolates of bacteria were obtained from 12 different black rot‐infected crucifer crops from 19 states of India; these were identified as Xcc based on morphology, hrpF gene and 16S rRNA gene based molecular markers and pathogenicity tests. Characterization of races was performed by using a set of seven differential crucifer hosts, comprising two cultivars of turnip (Brassica rapa var. rapa) and cultivars of Indian mustard (B. juncea), Ethiopian mustard (B. carinata), rapeseed mustard (B. napus), cauliflower (B. oleracea) and Savoy cabbage (B. oleracea var. sabauda). Races 1, 4 and 6 of Xcc were identified and, among these races, race 1 followed by race 4 dominated most of the states of India. Genetic diversity of the Indian isolates of Xcc was analysed using repetitive sequence‐based PCR (rep‐PCR) including primers for REP (repetitive extragenic palindromic), ERIC (enterobacterial repetitive intergenic consensus) and BOX (amplifying with BOX A1 R primer) repetitive elements. This method of fingerprinting grouped the isolates into 56 different DNA types (clusters) with a 75% similarity coefficient. Among these clusters, DNA types 22 and 53 contained two different races 1 and 4, whereas DNA type 12 contained races 1, 4 and 6. However, no clear relationship was observed between fingerprints and races, hosts or geographical origin. 相似文献