The in vitro developmental competence of oocytes from juvenile calves is related to follicular diameter

This study investigated the relationship between follicle size (FS) and developmental competence of calf oocytes. Cumulus-oocyte-complexes (COCs) from follicles>8 (L-COCs; n=19), 4-8 (M-COCs; n=54), and 2-3 mm (S-COCs; n=155) were recovered from non-stimulated 1-4 months old dairy calves post mortem and ex vivo (laparoscopy), and in parallel from slaughtered adult cows from follicles of identical size categories [>8 (n=91); 4-8 (n=138); 2-3 mm (n=193)]. Morphologically intact COCs were subjected to in vitro maturation, fertilization, and embryo culture. Cleavage rate (CR; 46 h post-insemination=p.i.), rate of morulae/blastocysts (M/Bl; day 7 p.i.), and blastocysts (Bl; day 9 p.i.) were recorded. FS had no effect on the CR in calves. However, calf L-COCs yielded the highest rates of M/Bl and Bl compared with the two other size categories (P<0.05). In contrast, calf S- and M-COCs gave similar rates of M/Bl, whereas the proportion of Bl was lowest for S-COCs (P<0.05). This was almost identical to findings in cows, except that the CR was highest for L-COCs and M/Bl yields were lowest for S-COCs (P<0.05). There were no differences between calf and cows with regard to CR for the respective FS categories. L-COCs from calves and cows yielded similar rates of M/Bl and Bl, whereas calf S- and M-COCs yielded lower rates of Bl than S- and M-COCs from cows and a lower rate of M/Bl when S-and M-COCs were analyzed as one group (P<0.05). Whereas the CR was similar in calves and cows, calf COCs yielded lower rates of M/Bl and Bl (P<0.05). In conclusion, the results show that the developmental competence of calf oocytes is higher in those derived from follicles larger than 8 mm, and thus are almost equally as competent as cow oocytes derived from follicles of identical size. This suggests that calf oocytes acquire developmental competence within the large follicle, potentially due to a process similar to prematuration of the oocyte in the adult cow. It is proposed that procedures that facilitate prematuration, such as "coasting" following a preceding superstimulation, might increase the developmental competence of calf oocytes.     

Genotyping of bovine Prototheca mastitis isolates from Poland

Bovine mastitis due to unicellular, achlorophyllous algae of the genus Prototheca is a serious and complex ailment that accounts for high economic losses in the dairy industry. Bovine protothecal mastitis has been almost exclusively associated with only one species Prototheca zopfii. Recently, based on the 18S rDNA sequence analysis, P. zopfii has been divided into three genotypes (1-3), of which P. zopfii genotype 3 has been given the status of a new species Prototheca blaschkeae sp. nov. The aim of this study was to investigate the genotypic composition of the population of P. zopfii bovine mastitis isolates from Poland, according to that threefold classification. Of the 44 isolates tested, 43 (98%) were identified as P. zopfii genotype 2. Only one isolate was assigned to the newly established P. blaschkeae species. These findings clearly show the predominance of the P. zopfii genotype 2 in the etiology of bovine mammary protothecosis in Poland, but also underline the involvement of P. blaschkeae in the disease. The study is the first to provide molecular characterization of Polish P. zopfii mastitis isolates, collected from different regions of Poland.     

Phenotypic evaluation of floral and flowering traits with relevance for hybrid breeding in wheat (Triticum aestivum L.)

Hybrid breeding is a promising approach to increase the yield potential in wheat (Triticum aestivum L.). The profitability of wheat hybrids highly depends on a cost‐efficient system for hybrid seed production for which an adequate outcrossing in the male pool is of utmost importance. Employing a set of 51 elite winter wheat lines, we developed and evaluated phenotyping methods for floral and flowering traits with relevance for improved cross‐pollination. We observed significant genotypic variances and high heritabilities for most traits, including important traits like pollen mass and anther extrusion. Our results suggest the utility of the developed phenotyping approaches for applied plant breeding and the potential of the traits to assist in the design of the male ideotype for increased cross‐fertilization.     

生物动力学品质检测技术简介——图像成形法

<正>在上世纪20年代一些测试方法开始发展,这些方法现在被统称为图像成形法(Picture forming methods)。其中最具代表意义的是氯化铜结晶法(copper chloride crystal-     

Nitrogen Fluxes of a Slope Mire in the German Harz Mountains

Nitrogen (N) fluxes of a slope mire in the German Harz Mountains were monitored to study the effect of increased N deposition on the N retention of the mire. In addition, the N content of mire pore water beneath different plant species was analyzed to assess N retention ability of plants. Atmospheric N deposition at the study site was 4.9?±?0.4 g N m^?2 year^?1 averaged for the study period of 2002 and 2003, with forest stand deposition being the largest share. Discharge was the main output pathway of N with a rate of 1.9?±?0.3 g N m^?2 year^?1. The mire showed a high N retention rate of 67%. Short-term N accumulation rate was 3.9 g N m^?2 year^?1. Differences in mire pore water N concentration under different vegetation cover indicate a lower N retention ability for ombrotrophic Sphagnum plants.     

Multiple-species conservation planning for European wetlands with different degrees of coordination

Selection and establishment of reserves was often done unplanned and uncoordinated between regions. Systematic conservation planning provides tools to identify optimally located priority areas for conservation. Planning for multiple species promises adequate provision for the needs of a range of threatened species simultaneously. Several studies apply the set-covering problem by minimizing resources for given conservation targets of multiple species. We extend this method by also considering different degrees of coordination in multiple-species conservation planning and representing reserve sizes endogenously. A deterministic, spatially explicit programming model solved with mixed integer programming is used to represent minimum habitat area thresholds for all included biodiversity features. The empirical model application to European wetland species addresses five different scenarios of coordination in conservation planning, including taxonomic, political, and biogeographical coordination of planning. Our approach illustrates and quantifies the efficiency of multi-species conservation activities. We show that maximum coordination in conservation planning enhances area efficiency by 30% compared to no coordination. Furthermore, strong coordination in conservation planning does not only reduce the area requirement, but synergy effects even enable the conservation features to achieve higher conservation objectives. Spatial subdivision of planning, however, leads to highest area requirements and less conservation target achievement.     

Prospects of breeding improved garlic in the light of its genetic and breeding systems

Summary Garlic is an obligate apomict which defies all possibilities of genetic improvement through recombinant breeding. Nevertheless, like other bulbous or tuberous plants garlic has accumulated a lot of variation in many characters. Evidence of hybridity exists even at the chromosomal level. Existence of this natural variation, even in respect of the plant part that is economically important, suggests the possibility of improvement. The prospects of exploiting this variation in the face of apomixis are discussed.     

Resveratrol induces apoptosis through ROS-dependent mitochondria pathway in HT-29 human colorectal carcinoma cells

trans-Resveratrol is a polyphenol found in blueberries, grapes, and wine with cancer chemopreventive properties. The low bioavailability of this compound enhances its concentration in the luminal content and becomes a potential chemopreventive agent against colon cancer. In the present study, the antiproliferative and pro-apoptotic effects on the human colorectal carcinoma HT-29 cells as well as the mechanisms underlying these effects were examined. Proliferation, cytotoxicity, and apoptosis were measured by fluorescence-based techniques. Studies of dose-dependent effects of trans-resveratrol showed antiproliferative activity with an EC 50 value of 78.9 +/- 5.4 microM. Caspase-3 was activated in a dose-dependent manner after incubation for 24 h giving an EC 50 value of 276.1 +/- 1.7 microM. Apoptosis was also confirmed with microscopic observation of changes in membrane permeability and detection of DNA fragmentation. The activity of trans-resveratrol on the mitochondria apoptosis pathway was evidenced by the production of superoxide anions in the mitochondria of cells undergoing apoptosis. In conclusion, trans-resveratrol inhibits cell proliferation without cytotoxicity and induces apoptosis in HT-29. Results of the present study provide evidence demonstrating the antitumor effect of trans-resveratrol via a ROS-dependent apoptosis pathway in colorectal carcinoma.     

Comparison of water‐retention functions obtained from the extended evaporation method and the standard methods sand/kaolin boxes and pressure plate extractor

Knowledge of the soil hydraulic functions is required for various hydrological studies and for the simulation of water and solute fluxes in unsaturated soils. Sand/kaolin boxes are frequently used to determine these properties in the low‐tension range. For higher tensions the pressure plate extractor is mainly applied. The extended evaporation method allows a more efficient determination of the water‐retention curve in an adequate range. Besides this method enables to quantify simultaneously the unsaturated hydraulic‐conductivity function. The objective of this study was to compare the water‐retention curves obtained from the standard methods (STM) with those determined with the extended evaporation method (EEM). A set of 90 natural soil samples of different texture and origin was analyzed, and the agreement between the methods was statistically evaluated. The average water‐content deviation (AWCD) of all samples was 1.83 vol.%, and the root mean square error (RMSE) 2.08 vol.%. The deviation of soil water‐storage capacity in the pore‐size classes 0–6, 6–30, 30–500, 500–1500 kPa varied between minimum –0.17 and 0.25 vol.% and maximum –2.89 and 2.36 vol.%, and confirmed the good comparability among the adopted methods. Systematic deviations between the methods were not found.