全文获取类型
收费全文 | 347篇 |
免费 | 19篇 |
专业分类
林业 | 14篇 |
农学 | 20篇 |
131篇 | |
综合类 | 15篇 |
农作物 | 21篇 |
水产渔业 | 27篇 |
畜牧兽医 | 120篇 |
园艺 | 5篇 |
植物保护 | 13篇 |
出版年
2023年 | 2篇 |
2022年 | 3篇 |
2021年 | 12篇 |
2020年 | 13篇 |
2019年 | 16篇 |
2018年 | 11篇 |
2017年 | 17篇 |
2016年 | 14篇 |
2015年 | 12篇 |
2014年 | 13篇 |
2013年 | 18篇 |
2012年 | 25篇 |
2011年 | 34篇 |
2010年 | 18篇 |
2009年 | 14篇 |
2008年 | 25篇 |
2007年 | 22篇 |
2006年 | 15篇 |
2005年 | 18篇 |
2004年 | 17篇 |
2003年 | 7篇 |
2002年 | 15篇 |
2001年 | 3篇 |
2000年 | 5篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1994年 | 1篇 |
1989年 | 3篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1977年 | 1篇 |
1975年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有366条查询结果,搜索用时 140 毫秒
51.
Michela Candelma Luisa Dalla Valle Sabrina Colella Alberto Santojanni Oliana Carnevali 《Fish physiology and biochemistry》2018,44(3):895-910
Teleosts have many spawning strategies and the hormonal control of gametogenesis is not well defined among the species or even, between sexes. To increase the knowledge of gonadotropin hormones, we studied the trend by gene expression of gonadotropin receptors in the follicles and testis at different maturity stages in the European hake (Merluccius merluccius), a multiple-spawning species. With this aim, fshr and lhr were sequenced, characterized, and their gene expression was quantified in oocytes and in testes at different maturity stages. The deduced amino acid sequences were used to phylogenetic studies and evidenced that both receptors are phylogenetically closed to other gadoid species. The gene expression of both receptors was poorly expressed in primary follicles, increased in vitellogenic follicles and to later decrease in hydrated oocytes. In testis, highest levels of lhr were detected during spermiation, while levels of fshr were constant. For the first time, a histological analysis was performed in European hake testes showing an unrestricted lobular testis. To better elucidate the mechanisms involved in the oogenesis of the European hake, the expression of estrogen receptor and cyp19a was also investigated displaying high levels in all classes of follicles. All these data allow to increase the knowledge on reproductive physiology of an important socioeconomical species and it seeks to shed more light on the role of the receptors here studied during gametogenesis of multiple-spawning fish. 相似文献
52.
Josefina Luisa De Paepe Alfredo Angel Bono Roberto Alvarez 《Arid Land Research and Management》2018,32(2):133-148
Soil available water capacity (AWC) is an important factor affecting soil productivity in semiarid and subhumid environments and is mainly determined by the soil textural composition. As the soils of these environments usually present fairly uniform textures across depth, we hypothesized that it would be possible to accurately estimate the whole-profile AWC using surface information. Our objective was to test this hypothesis in the Argentine Semiarid Pampas. Information was collected from 152 sites where AWC was measured in 20?cm layers up to a depth of 140?cm or up to the upper limit of the petrocalcic horizon, when present. In each case, whole profile AWC was estimated using a one-step and a two-step approach, comparing multiple regression and artificial neural networks as modeling techniques. Both modeling methods were effective (R2?>?0.76, P?0.05), however the former was chosen as no special software is required to run it, thus favoring simplicity. Models showed a strong interaction between surface AWC and soil depth and a simple nomogram was developed to estimate whole-profile AWC. Sampling and laboratory efforts should be significantly reduced using the model proposed in this paper. 相似文献
53.
Granum Espen Pérez-Bueno María Luisa Calderón Claudia E. Ramos Cayo de Vicente Antonio Cazorla Francisco M. Barón Matilde 《European journal of plant pathology / European Foundation for Plant Pathology》2015,142(3):625-632
European Journal of Plant Pathology - One of the most important soilborne diseases affecting avocado (Persea americana Mill.) crops is white root rot, caused by the fungus Rosellinia necatrix. In... 相似文献
54.
Isidro A. Prez M. Luisa Snchez M. ngeles García Beatriz de Torre 《Agricultural and Forest Meteorology》2009,149(10):1686-1692
CO2 is one of the most important gases linked to climate change. However, its ambient concentrations in a clean atmosphere have scarcely been investigated. In this paper a close relationship between CO2 concentrations and meteorological variables was proposed to establish atmospheric conditions linked to high CO2 values. A detailed 3-year campaign was carried out in a rural area with a CO2 continuous analyser and a RASS sodar for meteorological variables. Daily and yearly CO2 cycles were obtained. In addition, semi-hourly medians and quartiles were fitted to straight lines during the night time and daily maxima and minima were parameterised by means of a second order cylindrical model. Six meteorological variables were considered: wind speed, wind direction and temperature at one level and differences between them at two levels. Intervals were proposed from the highest CO2 concentrations during the night to build up a binary system of 64 meteorological classes. Analysis of both CO2 medians and meteorological classes enabled simplification of the system to four groups. The first group, characterised by high temperatures and drainage flow, was associated with detrended median concentrations above 15 ppm. Two groups of moderate concentrations were linked to thermal inversions and no prevailing wind directions. The final group corresponded to low concentrations associated with less than three meteorological variables with values linked to high concentrations. Only high directional shear and low wind speed were relevant in this group. This 4-group system was successfully used with the whole dataset. 相似文献
55.
Oliveiro Caetano de Freitas Neto Sonia Luisa Silva Lages Adriano Oliveira Torres Carrasco Angelo Berchieri Junior 《Tropical animal health and production》2009,41(8):1607-1614
We analyzed ostriches from an equipped farm located in the Brazilian southeast region for the presence of Salmonella spp. This bacterium was investigated in 80 samples of ostrich droppings, 90 eggs, 30 samples of feed and 30 samples of droppings
from rodents. Additionally, at slaughter-house this bacterium was investigated in droppings, caecal content, spleen, liver
and carcasses from 90 slaughtered ostriches from the studied farm. Also, blood serum of those animals were harvested and submitted
to serum plate agglutination using commercial Salmonella Pullorum antigen. No Salmonella spp. was detected in any eggs, caecal content, liver, spleen, carcass and droppings from ostriches and rodents. However,
Salmonella Javiana and Salmonella enterica subsp. enterica 4, 12: i:- were isolated from some samples of feed. The serologic test was negative for all samples. Good sanitary farming
management and the application of HACCP principles and GMP during the slaughtering process could explain the absence of Salmonella spp. in the tested samples. 相似文献
56.
Dermatophytosis is a common zoonotic disease, and one of its clinical presentations in the dog is nodular dermatophytosis (kerion). Because the infection is located within the dermis, routine diagnostic tests such as a Wood's lamp examination, microscopic examination of hair shafts for fungal elements and fungal culture can yield negative results. In such cases, histopathological examination with routine and special stains (periodic acid-Schiff, Gomori methenamine silver) is required to confirm the diagnosis. Nodular dermatophytosis in 23 dogs of different breed, age and sex with single or multiple nodules is described. Twelve dogs had a single nodule, and 11 dogs showed multiple lesions. Wood's lamp examination was negative in all cases. Microscopic examination of plucked hairs showed arthrospores in 8 of 23 cases. Skin scrapings in mineral oil looking for arthrospores and/or hyphae were positive in 12 cases. Impression smears of exudates were diagnostic in 21 of 23 cases (91%), showing arthrospores within fragments of hair shafts or free among neutrophils and macrophages (pyogranulomatous inflammation). Histopathology was performed in two cases. Fungal culture was positive for Microsporum canis in 16 dogs and for Microsporum gypseum in one dog. In six cases, the causative agent was not identified by fungal culture. All dogs were treated with systemic antifungal therapy and in eight cases with concurrent antibiotic therapy. Nodular dermatophytosis resolved in all dogs with the prescribed treatments within 4 to 8 weeks. Transmission to people or other pets in the home was not found. 相似文献
57.
58.
Passamonti F Veronesi F Cappelli K Capomaccio S Coppola G Marenzoni ML Piergili FD Verini SA Coletti M 《Comparative immunology, microbiology and infectious diseases》2010,33(1):73-83
Anaplasma phagocytophilum, the causative agent of granulocytic ehrlichiosis, affects several species of wild and domesticated mammals, including horses. In this work we compared direct and indirect methods to evaluate A. phagocytophilum presence in Central Italy: 135 sera were screened by IFA for A. phagocytophilum and other haemopathogens (Theileria equi and Babesia caballi). Each horse was also tested for A. phagocytophilum 16S rRNA with a nested-PCR technique. In order to examine the risk of A. phagocytophilum transmission, 114 ticks were examined for the presence of A. phagocytophilum by PCR targeting the 16S rRNA. The seroprevalence against A. phagocytophilum was 17.03% and 11 horses (8.14%) showed positive PCR results. The concordance rate of A. phagocytophilum detection between IFAT and PCR had a K value of 0.34. 相似文献
59.
Elsa Beltran Alberta De Stefani Jennifer Stewart Luisa De Risio Victoria Johnson 《Veterinary ophthalmology》2010,13(3):184-189
Mast cell tumors are found in most organs and tissues with variable biologic behavior in dogs. This case illustrates the clinical and magnetic resonance imaging (MRI) findings in a dog with disseminated mast cell tumor infiltrating the sphenoid bones. A 6‐year‐old male neutered Greyhound presented with a 3‐day history of acute onset of blindness. General physical examination was normal. Neurological examination revealed mildly disorientated mental status, absent menace response in both eyes, bilaterally decreased vestibulo–oculocephalic reflexes and absent direct and consensual pupillary light reflex in both eyes. An electroretinogram indicated normal retinal function in both eyes. A lesion involving the middle and rostral cranial fossa was suspected. Hematology and serum biochemistry were normal except decreased urea (1.2 mmol/L). MRI of the head revealed heterogeneous signal intensity of the sphenoid bones on T2‐weighted images and loss of their normal internal architecture. Cerebrospinal fluid analysis was normal. Abdominal ultrasound revealed hepatosplenomegaly and mesenteric lymphadenopathy. Fine needle aspirates were taken from the jejunal lymph nodes and the spleen. Results were consistent with disseminated mast cell tumor. The owner declined any treatment and the dog was euthanatized. Postmortem examination confirmed disseminated mast cell tumor affecting multiple organs, including the sphenoid bones. To our knowledge, this is the first case describing MRI features of disseminated mast cell tumor affecting the sphenoid bones and causing acute onset of blindness in a dog. 相似文献
60.
Díez-Municio M Montilla A Jimeno ML Corzo N Olano A Moreno FJ 《Journal of agricultural and food chemistry》2012,60(8):1945-1953
The production of new bioactive oligosaccharides is currently garnering much attention for their potential use as functional ingredients. This work addresses the enzymatic synthesis and NMR structural characterization of 2-α-D-glucopyranosyl-lactose derived from sucrose:lactose and sucrose:cheese whey permeate mixtures by using a Leuconostoc mesenteroides B-512F dextransucrase. The effect of synthesis conditions, including concentration of substrates, molar ratio of donor/acceptor, enzyme concentration, reaction time, and temperature, on the formation of transfer products is evaluated. Results indicated that cheese whey permeate is a suitable material for the synthesis of 2-α-D-glucopyranosyl-lactose, giving rise to yields around 50% (in weight respect to the initial amount of lactose) under the optimum reaction conditions. According to its structure, this trisaccharide is an excellent candidate for a new prebiotic ingredient, due to the reported high resistance of α-(1→2) linkages to the digestive enzymes in humans and animals, as well as to its potential selective stimulation of beneficial bacteria in the large intestine mainly attributed to the two linked glucose units located at the reducing end that reflects the disaccharide kojibiose (2-α-D-glucopyranosyl-D-glucose). These findings could contribute to broadening the use of important agricultural raw materials, such as sucrose or cheese whey permeates, as renewable substrates for enzymatic synthesis of oligosaccharides of nutritional interest. 相似文献